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OfflinePussyFart
Retired Cultivation Extrodinaire
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Registered: 04/08/12
Posts: 22,502
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Last seen: 7 months, 28 days
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Re: Attempting to grow-Cubensis B+ Exxperiment [Re: chen]
    #16785246 - 09/04/12 05:03 AM (12 years, 4 months ago)

Quote:

chen said:
When you see a brilliant white growth about the size of a penny, shake that shit up to distribute the spores, after this, colonisation will explode and should be finished over the next 2 weeks.




Dont do this, first of all ur not distributing spores, ur distibuting mycellim....by this time the spores have germinated. Shake at about 30% colonization and thats it. If u shake too early then it might stall. And never shake right after inoculation either.

Edited by PussyFart (09/04/12 05:04 AM)

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Offlinechen
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Last seen: 5 years, 10 months
Re: Attempting to grow-Cubensis B+ Exxperiment [Re: PussyFart]
    #16785260 - 09/04/12 05:07 AM (12 years, 4 months ago)

Quote:

Dont do this, first of all ur not distributing spores, ur distibuting mycellim....by this time the spores have germinated. Shake at about 30% colonization and thats it. If u shake too early then it might stall. And never shake right after inoculation either.





It's been proven that if you shake just after inoculation it hinders mycelium growth. When the mycelium is visible (ideally the size of a penny or slightly more) it increases it dramatically. I shake my bags when I can see visible growth and it has worked for me so far.


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OfflineHybridprX
Biodegrader of coir
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Registered: 01/29/08
Posts: 2,588
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Last seen: 7 years, 4 months
Re: Attempting to grow-Cubensis B+ Experiment [Re: PussyFart]
    #16785262 - 09/04/12 05:07 AM (12 years, 4 months ago)

Quote:

Notahacker420 said:
Quote:

Hellyen said:
Light is debatable. I think not necessary till u fruit. Anyway GL!




Light has been proven beneficial to the growth of mycellium during all stages.

Why would we be wasting our time telling noobs this every single day if it wasn't true?




Mycelium does not produce any ATP's from photons nor does it have a calvin cycle like plants.

It is NON-photosynthesis, anyone that took a elementary biology course in school would know this.

Why did RR go around and tell all of you guys this, beyond me....

Although I do agree with RR that if a cultivator is going to be fruiting bags of grain or pf cakes from the jars then light would benefit the cultivator (not the mycelium) by allowing a earlier pinset since it is one of the three pinning triggers (Drop in temp, light, drop in co2)


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OfflineHybridprX
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Re: Attempting to grow-Cubensis B+ Exxperiment [Re: PussyFart]
    #16785272 - 09/04/12 05:11 AM (12 years, 4 months ago)

Quote:

Notahacker420 said:
Quote:

chen said:
When you see a brilliant white growth about the size of a penny, shake that shit up to distribute the spores, after this, colonization will explode and should be finished over the next 2 weeks.




Dont do this, first of all ur not distributing spores, ur distributing mycellium....by this time the spores have germinated. Shake at about 30% colonization and thats it. If u shake too early then it might stall. And never shake right after inoculation either.




Again!

I'll fix you yet my friend.

After inoculation of spore solution the jars should be shaken vigorously to distribute the solution evenly throughout the jar.



3.5cc's of solution was used, jar was shaken to distribute, the jar was g2g'd seven days later.


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OfflinePussyFart
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Re: Attempting to grow-Cubensis B+ Exxperiment [Re: HybridprX]
    #16785281 - 09/04/12 05:18 AM (12 years, 4 months ago)

:facepalm: Whatever, Im tired of this, if u use 3.5ccs then yea i think it would be fine to shake right after inoculation...but if u use less that 1cc per jar(which i only use a couple drops per jar) then u take a chance on the spores being seperated too far apart to germinate....i dont care what u say 3ccs of spores is way too much when a couple drops does the same thing...but whatever.

Waste them spores boy! :lmao:


Light has been proven beneficial to the growth of mycellium during all stages.

And RR has said this many times, about light that is, but whatever, im done with this thread.

Mushrooms will colonize and fruit in the dark...but light helps colonization times and tells the mushies in which direction to grow. They will also be healthier.

Edited by PussyFart (09/04/12 05:20 AM)

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Offlinecloudpersona
I don't even...

Registered: 12/17/11
Posts: 1,285
Last seen: 7 years, 2 months
Re: Attempting to grow-Cubensis B+ Experiment [Re: HybridprX]
    #16785361 - 09/04/12 05:53 AM (12 years, 4 months ago)

Quote:

HybridprX said:
Quote:

Notahacker420 said:
Quote:

Hellyen said:
Light is debatable. I think not necessary till u fruit. Anyway GL!




Light has been proven beneficial to the growth of mycellium during all stages.

Why would we be wasting our time telling noobs this every single day if it wasn't true?




Mycelium does not produce any ATP's from photons nor does it have a calvin cycle like plants.

It is NON-photosynthesis, anyone that took a elementary biology course in school would know this.

Why did RR go around and tell all of you guys this, beyond me....

Although I do agree with RR that if a cultivator is going to be fruiting bags of grain or pf cakes from the jars then light would benefit the cultivator (not the mycelium) by allowing a earlier pinset since it is one of the three pinning triggers (Drop in temp, light, drop in co2)



Doesn't matter if it has photosynthesis. Do humans have photosynthesis? No, but we still benefit from light. Light regulates our circadian rhythm, produces vitamin d, among other benefits. So just because they don't produce energy from light doesn't mean they don't benefit from it.


--------------------
“The shaman is not merely a sick man, or a madman; he is a sick man who has healed himself.”
– Terence McKenna
“If the words ‘life, liberty, and the pursuit of happiness’ don’t include the right to experiment with your own consciousness, then the Declaration of Independence isn’t worth the hemp it was written on.”
-Terence McKenna

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Offlinechen
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Re: Attempting to grow-Cubensis B+ Exxperiment [Re: PussyFart]
    #16785449 - 09/04/12 06:59 AM (12 years, 4 months ago)

If you're worried about spores, make some LC. Problem solved.


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OfflinePussyFart
Retired Cultivation Extrodinaire
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Re: Attempting to grow-Cubensis B+ Exxperiment [Re: chen]
    #16785477 - 09/04/12 07:26 AM (12 years, 4 months ago)

Quote:

chen said:
If you're worried about spores, make some LC. Problem solved.



:facepalm:  What does LC solve exactly? LC from spores is a crapshoot because spores are never 100% clean and you cannot always see the contam in a LC.

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Offlinechen
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Re: Attempting to grow-Cubensis B+ Exxperiment [Re: PussyFart]
    #16787814 - 09/04/12 04:02 PM (12 years, 4 months ago)

Quote:

What does LC solve exactly?



LC has the miraculous ability to turn 1cc of spore solution in to a whole jug of usable liquid culture. For people short of spores, it solves a lot.


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OfflinePussyFart
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Re: Attempting to grow-Cubensis B+ Exxperiment [Re: chen]
    #16787939 - 09/04/12 04:25 PM (12 years, 4 months ago)

Quote:

chen said:
Quote:

What does LC solve exactly?



LC has the miraculous ability to turn 1cc of spore solution in to a whole jug of usable liquid culture. For people short of spores, it solves a lot.



:bathtub40lol:

Yea ok...if it doesnt contam....i will say it again...said it twice today  already...and like 50 times in the past 2 months.

LC FROM SPORES IS A CRAPSHOOT. SPORES ARE NEVER 100% CLEAN BECAUSE THE
CAPS WERE FRUITED IN THE OPEN AIR. YOU CANNOT ALWAYS TELL IF THE LC IS
CONTAMED JUST BY LOOKING AT IT, U HAVE TO SHOOT UP TEST JARS AND GROW
THEM OUT FIRST BEFORE U CAN CALL IT A CLEAN LC.

LC FROM SPORES SUCK JUST BECAUSE OF THE FAILURE RATE.(50/50 CHANCE)

GRAIN LC OR A LC FROM AN AGAR WEDGE IS DIFRERENT BECAUSE U KNOW THAT ITS CLEAN.

now is a 50/50 chance at failure worth it?

Edited by PussyFart (09/04/12 04:27 PM)

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OfflinePussyFart
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Re: Attempting to grow-Cubensis B+ Exxperiment [Re: PussyFart]
    #16787962 - 09/04/12 04:30 PM (12 years, 4 months ago)

just run a search for "contammed lc" for the past 3 years...read!

http://www.shroomery.org/forums/search.php

Edited by PussyFart (09/04/12 04:32 PM)

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Offlinecloudpersona
I don't even...

Registered: 12/17/11
Posts: 1,285
Last seen: 7 years, 2 months
Re: Attempting to grow-Cubensis B+ Exxperiment [Re: PussyFart]
    #16787977 - 09/04/12 04:33 PM (12 years, 4 months ago)

Quote:

Notahacker420 said:
CAPS WERE FRUITED IN THE OPEN AIR.



Challenge accepted, growing a cube in front of a flow hood. Then placing cap in PC'ed jar on PC'ed aluminum, in front of the hood. 100% clean spore print.


--------------------
“The shaman is not merely a sick man, or a madman; he is a sick man who has healed himself.”
– Terence McKenna
“If the words ‘life, liberty, and the pursuit of happiness’ don’t include the right to experiment with your own consciousness, then the Declaration of Independence isn’t worth the hemp it was written on.”
-Terence McKenna

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OfflinePussyFart
Retired Cultivation Extrodinaire
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Registered: 04/08/12
Posts: 22,502
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Re: Attempting to grow-Cubensis B+ Exxperiment [Re: cloudpersona]
    #16788006 - 09/04/12 04:37 PM (12 years, 4 months ago)

Quote:

cloudpersona said:
Quote:

Notahacker420 said:
CAPS WERE FRUITED IN THE OPEN AIR.



Challenge accepted, growing a cube in front of a flow hood. Then placing cap in PC'ed jar on PC'ed aluminum, in front of the hood. 100% clean spore print.



i guess the only way to tell then would be to skip the foil and just print str8 to agar no? that would eliminate a variable.

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Offlinecloudpersona
I don't even...

Registered: 12/17/11
Posts: 1,285
Last seen: 7 years, 2 months
Re: Attempting to grow-Cubensis B+ Exxperiment [Re: PussyFart]
    #16788024 - 09/04/12 04:39 PM (12 years, 4 months ago)

Quote:

Notahacker420 said:
Quote:

cloudpersona said:
Quote:

Notahacker420 said:
CAPS WERE FRUITED IN THE OPEN AIR.



Challenge accepted, growing a cube in front of a flow hood. Then placing cap in PC'ed jar on PC'ed aluminum, in front of the hood. 100% clean spore print.



i guess the only way to tell then would be to skip the foil and just print str8 to agar no? that would eliminate a variable.



Brilliant. Given 72 before printing, we could be sure the agar was clean too.


--------------------
“The shaman is not merely a sick man, or a madman; he is a sick man who has healed himself.”
– Terence McKenna
“If the words ‘life, liberty, and the pursuit of happiness’ don’t include the right to experiment with your own consciousness, then the Declaration of Independence isn’t worth the hemp it was written on.”
-Terence McKenna

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OfflinePussyFart
Retired Cultivation Extrodinaire
Male


Registered: 04/08/12
Posts: 22,502
Loc: Orbiting Earth
Last seen: 7 months, 28 days
Trusted Cultivator
Re: Attempting to grow-Cubensis B+ Exxperiment [Re: cloudpersona]
    #16788035 - 09/04/12 04:42 PM (12 years, 4 months ago)

Quote:

cloudpersona said:
Quote:

Notahacker420 said:
Quote:

cloudpersona said:
Quote:

Notahacker420 said:
CAPS WERE FRUITED IN THE OPEN AIR.



Challenge accepted, growing a cube in front of a flow hood. Then placing cap in PC'ed jar on PC'ed aluminum, in front of the hood. 100% clean spore print.



i guess the only way to tell then would be to skip the foil and just print str8 to agar no? that would eliminate a variable.



Brilliant. Given 72 before printing, we could be sure the agar was clean too.



:popcorn:

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InvisibleOeric McKenna
LIFE CAPS


Registered: 06/15/12
Posts: 5,318
Loc: Babylon Flag
Re: Attempting to grow-Cubensis B+ Exxperiment [Re: PussyFart]
    #16788335 - 09/04/12 05:47 PM (12 years, 4 months ago)

As for the lc debate. It used to be more popular a few yrs ago...
I can see the positives.
1) If proven clean, you have TONS of inoculant.(mass expansion potential)
2) you can shoot it into many different mediums.
3) tissue lc!
4) colonization speed
  I guess the downside is testing it. Its not so bad
Agar's baby bottle lc tek is genius I think


--------------------


spread love
love is everything
2013 finds
medicinal psilocybin tincture drops
cannabis pics

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Offlineshawnelmer824
INDIGO1111

Registered: 08/18/11
Posts: 16
Loc: NJ
Last seen: 12 years, 1 month
Re: Attempting to grow-Cubensis B+ Exxperiment [Re: chen]
    #16788599 - 09/04/12 06:41 PM (12 years, 4 months ago)

Quote:

chen said:
Sounds like you did everything right bro. Don't be worried, expect 2 weeks until you see any signs of mycelium growth. When you see a brilliant white growth about the size of a penny, shake that shit up to distribute the spores, after this, colonisation will explode and should be finished over the next 2 weeks. B+ is a forgiving strain, one of the easiest to work with imo...
Good luck :bigjoint:

Are you spawning to a mini mono? Grains don't perform on their own.



Ok thanks, Keeping fingers crossed. No, the bag has the substrate all through out it and at the top of th ebag is soil ph balanced and all, after colonization they root to the bottom of the soil and thats when you cut off the top of the bag and spray it with water, they pin out of the soil


--------------------
Names Shawn.  Im a full on spiritualist and Am Intrigued by Entheogens

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