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OfflineBrowser
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BRF Jars Contents Settling After PC
    #16775289 - 09/02/12 07:31 AM (12 years, 4 months ago)

I recently filled some BRF 1/2 pint wide neck glass jars. After I added the BRF mix (per RR video), I tapped the jar to settle the contents. I then added a 1/2 inch layer of dry verm. I tapped the jars down on the work surface and then skimmed the top dry verm layer,  flat with a flat edge. After pc'ing the foil covered jars for 2 hours I noticed when examining the cooled jars that the contents had settled by maybe 1/4 inch. I saw this because a couple of jar lids had unscrewed themselves and came off with the foil. I will put them on tighter next time and maybe rubber side down.

This morning I removed a contaminated jar from this batch

http://www.shroomery.org/forums/showflat.php/Number/16771971/page/1

When I opened it I could see that because of the settled contents the dry verm layer was lying on a slope within the jar and exposing the moist brf mixture on one side. This is where the contamination had started. I took great care to keep the jars level when handling, so it was strange to see the verm layer lying at an angle within the jar.

If the content hadn't settled so much then I don't think this would have happened. When I make a new batch of jars should I compress the brf mixture in the jar a little to try and prevent so much settling of the contents?

What do others do?

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OfflineSimpleFarmer
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Re: BRF Jars Contents Settling After PC [Re: Browser]
    #16775385 - 09/02/12 08:12 AM (12 years, 4 months ago)

Don't compact the sub, it will take for ever to colonize or will stall. You want it fluffy and airy

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Re: BRF Jars Contents Settling After PC [Re: SimpleFarmer]
    #16775435 - 09/02/12 08:30 AM (12 years, 4 months ago)

OK..I won't..maybe I should just tap it a little after the pc process to make sure that the dry verm layer is level. I was surprised that a couple of jar lids unscrewed. Must have been a lot of rattling inside the pc.

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Re: BRF Jars Contents Settling After PC [Re: Browser]
    #16775454 - 09/02/12 08:34 AM (12 years, 4 months ago)

I wouldn't ... Treat them like a middle school dance date not a prom date... Less stress you give them the less stress they will give you :wink:

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Re: BRF Jars Contents Settling After PC [Re: Browser]
    #16775457 - 09/02/12 08:35 AM (12 years, 4 months ago)

How high was the water in the PC?
I would guess like you said, they were knocking around
inside there. If you tapped the jars then odds are your substrate
was still fluffy, seeing how it still settled.. I've found that
no matter what the substrate settles some while PC'ing. Which
is why I choose to only steam them.


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Re: BRF Jars Contents Settling After PC [Re: DynGBreeD]
    #16775481 - 09/02/12 08:40 AM (12 years, 4 months ago)

I use fine verm for the top layer, but the coarse mixed with brf.  It works better IMHO


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Re: BRF Jars Contents Settling After PC [Re: DynGBreeD]
    #16775516 - 09/02/12 08:52 AM (12 years, 4 months ago)

The jars were spaced above the water.Maybe the lower layer of jars were just in the water. There was some moisture under the tin foil after the process was finished. I used micropore tape on the lids as well, so it kept things dry. This was my first ever batch.


Quote:

Which is why I choose to only steam them.




Can you explain what that process is?

Quote:

I use fine verm for the top layer, but the coarse mixed with brf.  It works better IMHO




Where do you find verm in different grades ie fine and coarse ?

Edited by Browser (09/02/12 08:53 AM)

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OfflineSimpleFarmer
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Re: BRF Jars Contents Settling After PC [Re: Browser]
    #16775521 - 09/02/12 08:53 AM (12 years, 4 months ago)


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Re: BRF Jars Contents Settling After PC [Re: SimpleFarmer]
    #16775648 - 09/02/12 09:34 AM (12 years, 4 months ago)

I found mine at a local home dept store.


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Re: BRF Jars Contents Settling After PC [Re: Browser]
    #16775857 - 09/02/12 10:39 AM (12 years, 4 months ago)

My jars settled a little bit too, but I just thought it was normal and went with it. Didn't do anything other than that and they turned out fine.


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Invisiblejenkinz
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Re: BRF Jars Contents Settling After PC [Re: Groc60]
    #16776277 - 09/02/12 12:17 PM (12 years, 4 months ago)

I did a couple BRF in a pressure cooker as well and it compressed everything so much that it went from completely full to the top to being able to see through the side below the lid about a 1/4". I don't see any contams yet, but colonization is the slowest I've ever seen. I will stick to steam sterilization. I can fit more jars in anyway.


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Re: BRF Jars Contents Settling After PC [Re: jenkinz]
    #16776335 - 09/02/12 12:31 PM (12 years, 4 months ago)

Quote:

I will stick to steam sterilization. I can fit more jars in anyway.




When you say "steam sterilization", do you mean in a boil pan of water ?

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Re: BRF Jars Contents Settling After PC [Re: Browser]
    #16776392 - 09/02/12 12:47 PM (12 years, 4 months ago)

I use a large stock pot with a canning rack. You could even use your pressure cooker, just don't allow it to build pressure. 90 minutes has given me 100% success so far. Probably made ~100 jars by now.

A huge stock pot is really cheap, 20-30 bucks. A comparably sized pressure cooker not so much...


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OfflineRevolutiontcm
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Re: BRF Jars Contents Settling After PC [Re: Browser]
    #16776398 - 09/02/12 12:49 PM (12 years, 4 months ago)

Or... Find a lid tek and skip the dry verm layer.

As i explained in a previous thread, micropore tape is a sufficient enough filter.

Just knock through it and retape before removing from the SAB. date and label.


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Invisiblejenkinz
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Re: BRF Jars Contents Settling After PC [Re: Revolutiontcm]
    #16776493 - 09/02/12 01:06 PM (12 years, 4 months ago)

That wouldn't solve the issue of the substrate being compressed tho.

I assume this is caused by something done wrong? The jars being air tight or maybe not being air tight? 10-15lbs of pressure on every single bit of substrate in that jar would have to do something either way right?


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Re: BRF Jars Contents Settling After PC [Re: jenkinz]
    #16780520 - 09/03/12 09:13 AM (12 years, 4 months ago)

I made another batch last night. I compressed them a little. I noticed just a little settling after pc'ing, so maybe I compressed them just enough. I inoculated them and I will see how I did....the results will speak for themselves and I will know if my technique is sound or a disaster.

Quote:

As I explained in a previous thread, micropore tape is a sufficient enough filter.

Just knock through it and retape before removing from the SAB. date and label.




Interesting do you put a bigger hole in the lid? I wonder if a taped hole gives less FAE than an open hole

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InvisibleDynGBreeD
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Re: BRF Jars Contents Settling After PC [Re: Browser]
    #16780639 - 09/03/12 09:51 AM (12 years, 4 months ago)

You don't need FAE, or want it in substrate jars. Just GE.
You don't need to re-tape the holes. But everyone has their
own ways. I just inoculate through the tape, vermiculite barrier
is enough.


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Re: BRF Jars Contents Settling After PC [Re: DynGBreeD]
    #16781092 - 09/03/12 11:50 AM (12 years, 4 months ago)

I used micropore tape during the pc process as well as tin foil and for some of the jars I scraped the tape away from the holes before inoculating and for the others I inoculated through the tape. It seemed that the jars with the scraped away tape colonized faster but my sample size was somewhat small to be really sure.

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Re: BRF Jars Contents Settling After PC [Re: DynGBreeD]
    #16781101 - 09/03/12 11:53 AM (12 years, 4 months ago)

Quote:

DynGBreeD said:
You don't need FAE, or want it in substrate jars. Just GE.
You don't need to re-tape the holes. But everyone has their
own ways. I just inoculate through the tape, vermiculite barrier
is enough.




You could, in theory, use tyvek/micropore tape for ge with the same 4 holes

and rtv silicon over a bigger hole for innoculations...  Ya?


--------------------


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No Pour AGAR Tek Easy COIR Trays! *
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"Yeah?  Well, DRACULA called... and he said he's coming over tonight, and I said OK!" :mastershake:

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Re: BRF Jars Contents Settling After PC [Re: TheApprentice]
    #16781132 - 09/03/12 11:59 AM (12 years, 4 months ago)

My 4 holes on those lids were 1/8th colonized just fine for me...


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