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InvisibleSporesAndSpores
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Bacterial endospores vs. Mold Spores
    #16720705 - 08/18/12 01:47 PM (11 years, 5 months ago)

I have been searching and I can't seem to find any information about the size, in microns of course, of bacterial endospores compared to mold spores. One site says mold spores are 10 microns at the lowest, another says 1, and I can't seem to find much info about the size of bacterial endospores of common bacteria at all.  What brings me to this question is my latest project. I've started 7 Shiitake WBS jars with polyfil filters, soaked for 24 hours and pressure cooked at 10 psi for 2 hours. I got a 100% success rate and germination in 7 days with 14 BRF cakes.  But only 3 of the grain jars have germinated in 10 days and the growth is ridiculously slow... Such seemingly constricted growth reminds me of bacterial contamination. There's no mold in any of my other jars, so maybe bacterial endospores could have found their way in through the polyfil.  I can smell the jar through the polyfil but it's hard to determine whether the musty smell is from the WBS or something more sinister...  If mold spores couldn't make it through the polyfil, could bacterial endospores make it in?  The syringes were fresh from a trusted vendor, so I'm really trying to narrow down what went wrong here, if anything.


Edited by SporesAndSpores (08/18/12 02:17 PM)


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InvisibleDoc_T
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Re: Bacterial endospores vs. Mold Spores [Re: SporesAndSpores]
    #16720720 - 08/18/12 01:50 PM (11 years, 5 months ago)

Endospores didn't fall through your polyfil.
They are real, but not the threat people make them out to be.
7-14 days to see growth from spores is normal.

Your grain prep procedure is a bit weird, but seems like it should work.
So the next guess is that there's a bad syringe. Drop a drop on a n agar dish and see what grows.

And can we get a pic of the jar?


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InvisibleSporesAndSpores
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Re: Bacterial endospores vs. Mold Spores [Re: Doc_T]
    #16720803 - 08/18/12 02:08 PM (11 years, 5 months ago)

These are two of the 7 jars, my bad I didn't even know what day it was  :gethigh:


By sporesandspores at 2012-08-18


By sporesandspores at 2012-08-18

They were inoculated on the 9th, so it's been 9 days.  I used the entire syringe in the process but I do have an LC of it so I could always find out for sure the hard way.  They could be fine, though I found it sort of concerning that I could smell the jar clearly through the polyfil, and barely at all through a jar that i recently inoculated with two layers of micropore tape.  Be careful to pick out the reflection of the flash and the mycelium.  The one on the right barely has anything going for it.  Just could have sworn my last Shiitake grow was so much quicker, but hey it's MS.


Edited by SporesAndSpores (08/18/12 02:18 PM)


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Re: Bacterial endospores vs. Mold Spores [Re: SporesAndSpores]
    #16720814 - 08/18/12 02:10 PM (11 years, 5 months ago)

For the future, use just a few drops per jar, spore water is potent.
A full syringe for 7 jars is terribly wasteful.

Jars don't look bad to me, I say wait a week and see what happens.
If they start to smell bad, then that's bacteria for sure.


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Re: Bacterial endospores vs. Mold Spores [Re: Doc_T]
    #16720835 - 08/18/12 02:15 PM (11 years, 5 months ago)



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Re: Bacterial endospores vs. Mold Spores [Re: zepski]
    #16720857 - 08/18/12 02:19 PM (11 years, 5 months ago)

Particle size isn't totally relevant with polyfil filters, there's a certain amount of static cling.
But bacterial endospores aren't flying through the air in most peoples' homes.
They really are sort of rare as a practical matter, though certainly there are plenty of them out there.

But if jars go bad after you shoot them, first most obvious suspect is the syringe.


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InvisibleSporesAndSpores
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Re: Bacterial endospores vs. Mold Spores [Re: zepski]
    #16720879 - 08/18/12 02:23 PM (11 years, 5 months ago)

Going by that list though it says Bacteria, not Bacterial Endospores.  And that would mean they're WAY smaller than mold spores O_O
But yeah they definitely shouldn't be flying through the air I filtered the air, vacuumed the whole area, drowned it in disinfectant, and wiped all the surfaces etc.  Now I just play the waiting game I suppose. That patience  :waitingpatiently:


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