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Offlinechineseguy
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Registered: 04/13/03
Posts: 151
Loc: Chongqing, China
Last seen: 13 years, 7 months
three questions
    #1664616 - 06/26/03 03:39 PM (13 years, 9 months ago)

1) how long does it take for peroxide to become inactive after mixing it with water 20:1- doesn't light break it down?

2) is it possible that using perox solution could actually do more harm than good by weakening the mycelium and making it more prone to contams?


3) this is unrelated, but when you use WBS as spawn to horse dung, is it common for the spawn (innoc points) to become contaminated themself while the dung is able to fight off the contams (cobweb). if this occurs, it is wise to chuck the whole thing, correct?


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OfflineMAIA
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Re: three questions [Re: chineseguy]
    #1666801 - 06/27/03 10:00 AM (13 years, 9 months ago)

1
IV. EFFECT OF A CATALYST

Materials

8 #2 ziploc bags containing:

2 vials containing 3% hydrogen peroxide

1 small scoop

1 container of manganese dioxide

1 small piece of paper towel

8 aluminum pie pans (from Section III)

8 slivers of potatoes in a jar of water

1 pair of tweezers

1 sample bottle of hydrogen peroxide (empty bottle is OK)


Ask students, What is a catalyst?

Accept logical responses.

(A catalyst is a substance that speeds up or slows down a chemical reaction but is not

changed itself by the reaction.)


Show the students the sample bottle of hydrogen peroxide.

Ask students what they know about hydrogen peroxide.

Accept logical responses.

(Some students will know that hydrogen peroxide is often put on cuts and that it

bubbles up. Someone might know that it is H2O2.)


Tell students that hydrogen peroxide (H2O2) will chemically decompose (break down) into

oxygen gas (O2) (yes, the glowing splint test will work!) and water (H2O). Hydrogen peroxide is sold in brown bottles because it will decompose in the presence of light. Hydrogen peroxide bottles are dated because even in a brown bottle, hydrogen peroxide will decompose over time.


Tell students that the decomposition of hydrogen peroxide will speed up if a catalyst is added to the hydrogen peroxide.



Give each group the following:

1 #2 ziploc bag containing:

2 vials containing 3% hydrogen peroxide

1 small scoop

1 container of manganese dioxide

1 small piece of paper towel


One VSVS volunteer should use the tweezers to place a sliver of potato on each group?s small piece of towel.


Have each group do the following:

? Place the two vials of hydrogen peroxide on the aluminum pie pan from Section III.

? Observe the hydrogen peroxide to see that nothing is happening. No bubbles are

being given off.

? Place the container of manganese dioxide where all group members can observe it.


Have each group:

? Take the cap off the two vials.

? Place a small scoop of manganese dioxide in one vial and a sliver of potato in the other vial.

? Observe what happens and record observations.


Ask students, What happened?

(Bubbles are observed in both vials. However, the manganese dioxide and the potato sliver still look the same. They are both catalysts that speed up the decomposition of hydrogen peroxide.)

Have students replace screw caps on the vials. Put them in the trash bag and return with kit to VSVS lab.


EXPLANATION: A catalyst speeds up a chemical reaction without being used up.. The catalyst does this without undergoing permanent change itself, so it can act over and over again. Many chemical reactions are slow because they involve several intermediate steps that are of high energy. A good analogy is the difference between driving over a mountain or driving through a tunnel in the mountain. It takes less time to drive through a tunnel than over the top of the mountain. The regular chemical reaction goes through an intermediate state of high energy like going over a mountain while the catalyst provides a new pathway of lower energy for the chemical reaction, similar to going through a tunnel in the mountain.


In the present case, the manganese dioxide catalyzes the decomposition of hydrogen peroxide by a series of fast reactions that change manganese dioxide to manganese oxide and then back to manganese dioxide. Since the manganese dioxide speeds up the decomposition of the hydrogen peroxide and is regenerated during the course of the reaction, it is a catalyst.


Enzymes are biological catalysts. The catalase in the potato slice is an example of an enzyme. Enzymes bind their substrates (in this case H2O2) into a cavity before catalyzing a reaction. Once the substrate is in the cavity, binding between the enzyme and the substrate weaken substrate bonds, making it easier for new bonds to form. The best analogy is the Venus flytrap. This plant traps an insect by folding around it. The folding is triggered by the presence of the insect, just as the folding of the enzyme is triggered by the presence of the substrate. After the catalyzed reaction is complete, the enzyme opens up again to let the products leave and to prepare for the next substrate molecule. Your body has thousands of enzymes that catalyze reactions, including catalase in the blood that catalyzes the breakdown of hydrogen peroxide that is formed in some biological reactions and must be decomposed to avoid harmful effects on the body.


Another example of an enzyme at work is the breakdown of starches to simple sugars. The next time you are chewing a cracker, see if you can tell whether the initial salty taste turns to a sweet taste. The saliva in your mouth causes a chemical breakdown of the starch in the cracker as the cracker is chewed by the mouth. The enzyme ptyalin begins breaking down the starch in the cracker to glucose so the cracker begins to taste sweet.

2
High concentrations of H2O2 may harm mycelium, if the recovery takes too long, contaminants might have a chance to settle.

3
If after spawning the result is contamination free, you are ok to continue. Cobweb is that kind of contam you can get rid off by treating it before making the transfer. Dunking in H2O2/H2O solution at this stage is a good solution, otherwise you have more chances for a return.
If you can't treat it chucking the whole thing is a good idea, sure.

MAIA


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Spiritual being, living a human experience ... The Shroomery Mandala



Use, do not abuse; neither abstinence nor excess ever renders man happy.
Voltaire


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Offlinechineseguy
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Re: three questions [Re: MAIA]
    #1667310 - 06/27/03 04:03 PM (13 years, 9 months ago)

good info MAIA.

how would one go about dunking a contaminated and unclolonized mixture of dung and spawn since its not a whole mass like a cake? would treating the contaminated spots with a dropper work?

thanks


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OfflineMAIA
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Re: three questions [Re: chineseguy]
    #1669141 - 06/28/03 11:58 AM (13 years, 8 months ago)

You have to remember that a cake is treated as a whole, a chunk, a mass affected only on the outside, so the compromised mycelium, the one being affected by peroxide is a small surface compared to the total surface of several small chunks of mycelium serving as spawn. Dunking is not a good idea in the case you describe.
If you're trying to fight contamination, i would say it's a shot in the dark. Un colonized and contaminated substrates after transfers can be a time a waste of time if you plan to treat them. It depends on the contaminant, h2o2 is good against cobweb and sometimes against trich and it's good as prevention when used to mist as a diluted solution but that's it, i mean, sometimes it really doesn't worth the hassle.

MAIA


--------------------
Spiritual being, living a human experience ... The Shroomery Mandala



Use, do not abuse; neither abstinence nor excess ever renders man happy.
Voltaire


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Amazon Shop for: ½ Pint Jars, Wild Bird Seed

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