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Amazon Shop: ½ Pint Jars, Laminar Flow Hood

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Invisiblemikey
Stranger

Registered: 06/04/03
Posts: 548
Syringe
    #1664310 - 06/26/03 01:36 PM (14 years, 3 months ago)

if i follow the spore works faq with syringes and it doesnt work what else could my problem be i have been very careful with sterilization and clean work area


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Offlineknurd
enthusiast

Registered: 11/17/01
Posts: 330
Last seen: 1 year, 3 months
Re: Syringe [Re: mikey]
    #1664315 - 06/26/03 01:37 PM (14 years, 3 months ago)

What's the problem?


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PLEASE DON'T PM ME FOR FREE SPORES I GAVE AT THE OFFICE

Now I will trade when I can


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Offlineneutralizer
Spiritual beinghaving a Humanexperience
Registered: 06/17/03
Posts: 635
Loc: This Planet Earth
Last seen: 10 years, 8 months
Re: Syringe [Re: mikey]
    #1664316 - 06/26/03 01:38 PM (14 years, 3 months ago)

How long has it been? What temp are the jars at? I'm not familiar with the spore works faq, is it similar to the PF-Tek?


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There are things known, and there are things unknown, and in between are the doors - Morrison


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Invisiblemikey
Stranger

Registered: 06/04/03
Posts: 548
Re: Syringe [Re: knurd]
    #1664317 - 06/26/03 01:38 PM (14 years, 3 months ago)

i keep trying diffent ways to make syringe i have tried every way under the sun but none of them ever work


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Invisiblemikey
Stranger

Registered: 06/04/03
Posts: 548
Re: Syringe [Re: mikey]
    #1664328 - 06/26/03 01:42 PM (14 years, 3 months ago)

yea ah at like 80 f


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Offlineneutralizer
Spiritual beinghaving a Humanexperience
Registered: 06/17/03
Posts: 635
Loc: This Planet Earth
Last seen: 10 years, 8 months
Re: Syringe [Re: mikey]
    #1664362 - 06/26/03 01:53 PM (14 years, 3 months ago)

I looked on their FAQ and couldn't find instructions on how to make the syringes, what exactly are you doing? Can you give us a link to the directions?


--------------------
There are things known, and there are things unknown, and in between are the doors - Morrison


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InvisibleMagashM
Da Bud Guru
 User Gallery

Registered: 07/25/02
Posts: 5,875
Loc: Near Hilo
Trusted Cultivator
Re: Syringe [Re: neutralizer]
    #1664377 - 06/26/03 02:04 PM (14 years, 3 months ago)

Bring some water to boil on the stove and let it boil about 10 minutes.
Suck the boiling water up to a fresh syringe.
If the syringe was used before, sterilize the allready filled syringe in a PC for 15 minutes.
Put syringe aside until cool.
Following tasks should be idealy prepared in front of a flow hood, but a reasonably cleaned bathroom will do:
Prepare the print, a shot glas and the alcohol lamp.
Rinse the clean shotglass with isopropanol or rubbing alcohol and let it evaporate a few seconds.
Bring the needle of the syringe to red glow above the alcohol lamp.
Squirt 1/2 ml of water out of the syringe to cool the needle and create some airspace in the syringe so later you can shake the spores.
Shake of the eventual drop on top of the needle off so the print won?t become wet.
Scrape some spores with the tip of the needle into the shotglas.
Add a small amount of water from the syringe, stir and suck it back into syringe.
Give the syringe a vigorous shake, let the spores rehydrate for a few hours and inoculate







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All creatures tremble when faced with violence. All creatures fear death, all love life. If we can only see ourselves in others, then how could we possibly hurt another creature?


:growingweed: Join us at the Growery! :growingweed:


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OfflineDannyBoy
ShroomEnthusiast

Registered: 01/31/03
Posts: 429
Loc: South East, U.S.A.
Last seen: 13 years, 1 month
Re: Syringe [Re: Magash]
    #1664385 - 06/26/03 02:09 PM (14 years, 3 months ago)

^^^
:loveeyes:  :nut:  :rolleyes:  - show off :smile:


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"It has been said that kids say the darndest things, but so would you if you had no education"


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InvisibleMagashM
Da Bud Guru
 User Gallery

Registered: 07/25/02
Posts: 5,875
Loc: Near Hilo
Trusted Cultivator
Re: Syringe [Re: DannyBoy]
    #1665293 - 06/26/03 07:41 PM (14 years, 3 months ago)




--------------------
All creatures tremble when faced with violence. All creatures fear death, all love life. If we can only see ourselves in others, then how could we possibly hurt another creature?


:growingweed: Join us at the Growery! :growingweed:


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Offlinekukusz
Owner of belowhot monkey!

Registered: 05/10/03
Posts: 519
Loc: East Coast
Last seen: 7 years, 9 months
Re: Syringe [Re: Magash]
    #1665301 - 06/26/03 07:42 PM (14 years, 3 months ago)

Seriously stop showing off!  Holy shit!

:shocked:


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All of my posts are for discussion purposes only. All posts concerning cultivation of mushrooms refer to legal gourmet mushrooms only. Yes, I support monkey-human relationships.


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InvisibleLiL_KuSsH
Lost In The Trip

Registered: 05/07/03
Posts: 3,001
Deleted [Re: Magash]
    #1665302 - 06/26/03 07:43 PM (14 years, 3 months ago)

Post deleted by administrator


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InvisibleMagashM
Da Bud Guru
 User Gallery

Registered: 07/25/02
Posts: 5,875
Loc: Near Hilo
Trusted Cultivator
Re: Syringe [Re: DannyBoy]
    #1665328 - 06/26/03 07:54 PM (14 years, 3 months ago)



--------------------
All creatures tremble when faced with violence. All creatures fear death, all love life. If we can only see ourselves in others, then how could we possibly hurt another creature?


:growingweed: Join us at the Growery! :growingweed:


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OfflineDannyBoy
ShroomEnthusiast

Registered: 01/31/03
Posts: 429
Loc: South East, U.S.A.
Last seen: 13 years, 1 month
Re: Syringe [Re: Magash]
    #1665436 - 06/26/03 08:53 PM (14 years, 3 months ago)

LMAO....Your crazy Magash....your such a picture whore and your damn good at it too!


--------------------
"It has been said that kids say the darndest things, but so would you if you had no education"


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OfflineDannyBoy
ShroomEnthusiast

Registered: 01/31/03
Posts: 429
Loc: South East, U.S.A.
Last seen: 13 years, 1 month
Re: Syringe [Re: DannyBoy]
    #1665448 - 06/26/03 08:59 PM (14 years, 3 months ago)

Check out my little pic underneath my username...heh heh


--------------------
"It has been said that kids say the darndest things, but so would you if you had no education"


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InvisibleLiL_KuSsH
Lost In The Trip

Registered: 05/07/03
Posts: 3,001
Deleted [Re: DannyBoy]
    #1665532 - 06/26/03 09:34 PM (14 years, 3 months ago)

Post deleted by administrator


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Amazon Shop: ½ Pint Jars, Laminar Flow Hood

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