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Amazon Shop: ½ Pint Jars, Agar, Vermiculite

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InvisibleMOTH
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Registered: 06/06/03
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Mega spores
    #1661398 - 06/25/03 02:51 PM (14 years, 29 days ago)

Well, I think I might have made a mistake when innoculating my cakes today. Trying to get the hang of handling the syringe, I accidently squirted much much more spore liquid into my jar then was recommended by the PF tek. This happened in about 2-3 jars or so before I finally thought I knew what was doing. What is the possible impact of this on colonization?


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InvisibleLiL_KuSsH
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Registered: 05/07/03
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Deleted [Re: MOTH]
    #1661404 - 06/25/03 02:53 PM (14 years, 29 days ago)

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OfflineAmuro
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Re: Mega spores [Re: LiL_KuSsH]
    #1661423 - 06/25/03 03:00 PM (14 years, 29 days ago)

the impact will be as stated above: they will colonize faster


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InvisibleMOTH
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Re: Mega spores [Re: Amuro]
    #1661444 - 06/25/03 03:06 PM (14 years, 29 days ago)

Um...probably 1/2 CC to a entire CC in *one* hole in the lid, and then I proceeded to accidently do the same thing to the other holes.  Grrr...finally about the 4th jar I had a feel for what I was doing and did like the tek suggested.  :tongue:


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InvisibleAlkaloids
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Re: Mega spores [Re: MOTH]
    #1661452 - 06/25/03 03:10 PM (14 years, 29 days ago)

If you put a very large amount in, then it might retard the growth or possibly even stop it. This will be due to the fact that there might be too high of a moisture content. Too much moisture=favorable conditions for contaminants
hope it turns out alright


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OfflineMAGICSNOOP
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Registered: 03/31/03
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Re: Mega spores [Re: Alkaloids]
    #1661505 - 06/25/03 03:30 PM (14 years, 29 days ago)

ITS GONA GROW FASTER U NEED MORE THAN 4 CC TO FUCK UP THE WATER CONTENT oops sorry bout yellin


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InvisibleAlkaloids
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Re: Mega spores [Re: MAGICSNOOP]
    #1661521 - 06/25/03 03:36 PM (14 years, 29 days ago)

actually that is not entirely accurate. If the substrate was already quite wet 4CC might cause pooling in the jar. Free standing water is a big no-no in this case.
I don't work with pf-tek so I am not sure what kind of leeway you have with moisture content, but from working with grain/agar i can tell you that water content is one of the most important factors in determining how fast/well your substrate is going to be colonized.


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InvisibleMOTH
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Re: Mega spores [Re: Alkaloids]
    #1661635 - 06/25/03 04:28 PM (14 years, 29 days ago)

Well, I didn't notice any pooling at all in the jars so maybe I'm still good. It just looks damp or moist inside. Agh...learning experience I guess.


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InvisibleMagashM
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Re: Mega spores [Re: MOTH]
    #1662184 - 06/25/03 07:18 PM (14 years, 29 days ago)

If you see any pooling turn the jar over and let the moisture run into the verm layer. Not wise but it will save you in a pinch.


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Amazon Shop: ½ Pint Jars, Agar, Vermiculite

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