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eLeSDenes
Mycelium Expander




Registered: 05/25/11
Posts: 955
Last seen: 1 month, 4 days
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Cloning from tissue to agar
#16605604 - 07/28/12 11:46 AM (11 years, 9 months ago) |
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Hello fellaz,
I'm just thinking if a cottony growth mycelium can be good fruiter. I cloned a nice looking cluster to agar plate and its rather cottony than rhizo. I was thinking if maybe there are some substrains in it or something. Should I try to isolate or just wait till the petris are full and try to grow them and lets see what happens? thanks for the advice
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Doc_T
Random Dude




Registered: 03/06/09
Posts: 42,395
Loc: Colorado
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Re: Cloning from tissue to agar [Re: eLeSDenes]
#16605679 - 07/28/12 11:56 AM (11 years, 9 months ago) |
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A cottony piece (tomentose) can be a good fruiter. Depends on a lot of factors, the same culture could be rhizomorphic on a different medium. At any rate it's not an immediate disqualifier. And you might need to do a few transfers first too.
-------------------- You make it all possible. Doesn't it feel good?
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eLeSDenes
Mycelium Expander




Registered: 05/25/11
Posts: 955
Last seen: 1 month, 4 days
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Re: Cloning from tissue to agar [Re: Doc_T]
#16605738 - 07/28/12 12:05 PM (11 years, 9 months ago) |
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thanks Doc. I knew i could trust you:D yeah i think i'm gonna do couple of transfers. I hope its gonna work and i can achieve wall to wall flushes:D I tried to isolate from spores but i could not really grow the rhizo sectors. for some reason only the cottony grew. I used Light malt extract (10g), 10g agar, 500ml water. but tried with 5g LME, 10g agar, 500ml water as well. Maybe with different media? what do oyu recommend?
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Doc_T
Random Dude




Registered: 03/06/09
Posts: 42,395
Loc: Colorado
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Re: Cloning from tissue to agar [Re: eLeSDenes]
#16605992 - 07/28/12 12:48 PM (11 years, 9 months ago) |
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Do a few transfers, see where that gets you. A tissue clone generally is a pretty good culture.
-------------------- You make it all possible. Doesn't it feel good?
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Mateo
High on LIFE!



Registered: 06/24/11
Posts: 2,695
Loc: North Europe
Last seen: 10 days, 5 hours
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Re: Cloning from tissue to agar [Re: Doc_T]
#16634496 - 08/02/12 03:27 PM (11 years, 9 months ago) |
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Isn't the only way to know if a mono-culture mycelium (from a cloned mushroom) are a good fruiter to actually do a testgrow? I guess you could judge some things on agar as how aressive colonizer it is, mycelium apperance (cottony, rizo).
Good luck with your mono-culture mycelium. I hope it's a good fruiter, contaminent resistant, potent and a agressive colonizer.
/Mateo
-------------------- A wise rat has many holes
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OICU812
NC Tree Farm owner



Registered: 11/06/11
Posts: 1,064
Loc: Foothills of NC
Last seen: 3 years, 7 months
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Re: Cloning from tissue to agar [Re: Mateo]
#16635774 - 08/02/12 07:26 PM (11 years, 9 months ago) |
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Your LME looks a little light to me, and there are no other nutrients in your agar formula. Sometimes the mycelia gets cottony because it doesn't like the menu. Here are some new recipes to try on the mycelia when you transfer:
FastFred's Media Cookbook
Good luck, and report back your results please.
-------------------- -------------- "Democracy is two wolves and a lamb voting on what to have for lunch. Liberty is a well-armed lamb contesting the vote!" --Benjamin Franklin "Those who give up liberty for security won't have, or deserve, either.". . . Benjamin Franklin ----> Read: The Fight of our Lives - Defeating the Ideological War Against the West - by Victor Davis Hanson
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eLeSDenes
Mycelium Expander




Registered: 05/25/11
Posts: 955
Last seen: 1 month, 4 days
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Re: Cloning from tissue to agar [Re: OICU812]
#17372783 - 12/11/12 07:58 AM (11 years, 5 months ago) |
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thank you very much for your advice. was very busy lately so i could not do any transfer. i'm making some petris in the coming days and will post my results! thank you very much!!
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mycoelf
Agent Of Chaos



Registered: 06/26/09
Posts: 555
Loc: hyperspace
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Re: Cloning from tissue to agar [Re: eLeSDenes]
#17389057 - 12/13/12 11:01 PM (11 years, 5 months ago) |
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I would advise to pour an MEA with 1/2 the sugar, if there are RIZO potential in a strain it is encouraged by the low nutrients to run, and when it runs is where the good sectors show up.
-------------------- Mycoelf Sterility is a process that can be likened unto infinity, which is a long walk, the closer to the end you start before beginning, the more achievable the goal of infinity becomes. Remember, cleanliness in next to goddessness
      
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