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Offlinemetaophion
stranger thanstrange
Registered: 06/23/03
Posts: 28
Loc: Seminyak, Bali - Indonesi...
Last seen: 12 years, 7 months
*NEW* Alien Broth Tek --- with pictures!
    #1656561 - 06/23/03 08:12 PM (13 years, 5 months ago)

Alien Broth Tek



Hello fellow travelers
in space. I feel I should share a wonderful story:


I have a friend that was
abducted by aliens and was given the following instructions and photographs. It
appears to be instructions for the propagation and archival of live fungal
mycelium. Much of it taken directly from the industrial processes of this
strange alien race. I suggest that this process be used for research purposes
only and applies to many fungal species, many of which are inedible.


Now that we have dispensed
the formalities:


Basic Recipe:
1.50
Liters of Spring Water (for mineral content)
35 grams Maltose
Monohydrate
32 grams Dextrose (anhydrous glucose)

3 grams Thiamine Hydrochloride (vitamin B1)



Required Items:

36 Septa Vials (40ml. - there are 72 in a standard "field pack")
Sterile 3cc. Syringes with luer-lock tip

22 gauge 1.5" Sterile Hypodermic Needles (luer-lock)


Possible Substitutions:

High Maltose Refined Rice Syrup Solids in place of Maltose and Dextrose
(available from your local home brew supply) and 1cc. Insulin syringes for 3.cc
luer-lock syringes.



Instructions:







Weigh and mix maltose, glucose and thiamine with spring water. Using a sterile
60cc. syringe add 30 ml. of broth solution to your 40 ml. septa vials.










Cook these in a pressure cooker at 15 P.S.I. for 20-25
minutes.







Allow to cool to room temperature an decompress
slowly to avoid boil over. Remove lid and seal vials tightly and immediately.
Now here is the tricky part. The aliens did a sterile loop from active spores in
sterile alien broth solution to get my initial mycelium. Technically any live
mycelium added to broth solution should work once it has been established that
the mycelium is live and free of other organisms.  Once these vials had
nothing but mycelium growing in them, they shook them up and distributed 0.1cc.
(10 units) to each of my autoclaved and sealed 40 ml. septa vials. Visible
growth appears in 2-3 weeks with a good "snot wad" forming in 1-2 months.










Once growth is established in the septa vials use PF,
Hawk or other growth medium method.  Shake vials and inject with a long
needle 1cc. of solution into your media to initiate rhyzomorphic mycelium growth
(or dump it in a sprayer, shake it up and visit your local cow fields to spray
new cow patties).








Other Information:


The aliens have tried many broth combinations,
including a straight glucose to make both 5% and 10% solutions. They found the
above formula to be the most prolific. Although the mycelium does grow in
nothing but glucose and water, the growth was slow and small and barely at all
in the 10% solution. If you use rice syrup solids make a 5% solution (50 grams
per liter of water).





--------------------



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OfflineRaadt
nicht

Registered: 06/07/02
Posts: 2,104
Loc: azurescending
Last seen: 8 years, 10 months
Re: *NEW* Alien Broth Tek --- with pictures! [Re: metaophion]
    #1656605 - 06/23/03 08:24 PM (13 years, 5 months ago)

good post. Thanks a lot =)

Very informative, do you have information for people on the vials - where to get? I know it'll be asked. I have a supply for vacutainers which I think I could use in this application. I will try.


--------------------
Raadt

-- The information I provide is only information from readings, growing of gourmet mushrooms, and second hand stories--


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Offlinemetaophion
stranger thanstrange
Registered: 06/23/03
Posts: 28
Loc: Seminyak, Bali - Indonesi...
Last seen: 12 years, 7 months
Re: *NEW* Alien Broth Tek --- with pictures! [Re: Raadt]
    #1656839 - 06/23/03 10:14 PM (13 years, 5 months ago)

Vacutainers would work fine. A google search for Septa Vial 40 will reveal many sources. Although mycelium will grow in an anaerobic environment, they grow faster in the presence of regular atmosphere. Strong consideration has been given to compressing the vials with a nitrox diving gas mixture, or even pure 02 (careful!) if you have access to medical or welding gasses. Hence the suggestion of allowing the vials to cool to room temperature instead of sealing hot and creating vacuum. Septa Vials can be autoclaved over and over, and the injection sites last for many punctures if a high gauge needle is used.


--------------------



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OfflineStatic
Can you hear menow?

Registered: 06/15/03
Posts: 113
Last seen: 12 years, 10 months
Re: *NEW* Alien Broth Tek --- with pictures! [Re: Raadt]
    #1656865 - 06/23/03 10:26 PM (13 years, 5 months ago)

This is a double post , though be it interesting.


--------------------
Astronauts get all the tang they want.


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