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InvisiblePhoenix Crash
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Agar & PE Swab Questions...
    #16540033 - 07/16/12 03:03 PM (11 years, 7 months ago)

Ok, so this is my first attempt at agar. I got some PE swabs from a trade the other day and made my first agar dishes yesterday.

Now, the question: How do I get the spores from the swab onto the agar?
Do I simply rub the swab across the surface? Or, do I need to put a drop of distilled water on the swab before I rub it on the surface?


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Why is pot against the law? It wouldn't be because anyone can grow it, and therefore you can't make a profit off it, would it? - Bill Hicks



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Invisible5is
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Re: Agar & PE Swab Questions... [Re: Phoenix Crash]
    #16540168 - 07/16/12 03:33 PM (11 years, 7 months ago)

Just rub it the spores across the surface of the agar.


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OfflineWing
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Re: Agar & PE Swab Questions... [Re: 5is]
    #16540218 - 07/16/12 03:43 PM (11 years, 7 months ago)

Personally I take some sterile scissors and cut a piece of the spored cotton away from the swab, leaving most of the spores on the swab. Then I take the piece I cut off and grab it with sterile tweezers and swipe it across the agar.

This avoids getting agar on your swab and having spores germinate on your swab


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InvisibleDoc_T
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Re: Agar & PE Swab Questions... [Re: Wing]
    #16540257 - 07/16/12 03:50 PM (11 years, 7 months ago)

I tap it like flicking ash off a cigarette.


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InvisiblePhoenix Crash
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Re: Agar & PE Swab Questions... [Re: Wing]
    #16540271 - 07/16/12 03:53 PM (11 years, 7 months ago)

Everything I seemed to find using the search function mentioned low success rates with rubbing/swiping the swab across the agar. I have several swabs and plates, so I guess I could try tapping the swab like a cigarette over the plate too.

I appreciate the replies guys. Is there anyone out there that has successfully gotten a PE swab to germinate on agar?


--------------------
Why is pot against the law? It wouldn't be because anyone can grow it, and therefore you can't make a profit off it, would it? - Bill Hicks



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OfflineSoreSpore
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Re: Agar & PE Swab Questions... [Re: Wing]
    #16540305 - 07/16/12 04:00 PM (11 years, 7 months ago)

Quote:

Phoenix Crash said:
Is there anyone out there that has successfully gotten a PE swab to germinate on agar?



I have successfully germinated spores from PE and APE on agar over 100 times.

Quote:

Wing said:
Personally I take some sterile scissors and cut a piece of the spored cotton away from the swab, leaving most of the spores on the swab. Then I take the piece I cut off and grab it with sterile tweezers and swipe it across the agar.

This avoids getting agar on your swab and having spores germinate on your swab



This is exactly how I do it.

Wherever you read of poor success rates rubbing or swiping the cotton swab across the plate, were they using a glovebox or a flowhood? I have a laminar flowhood and have never had an issue with spores germinating from a swab. Speaking from a completely neutral party, how would viable spores not germinate on any properly prepared media, regardless of the device used to get them there (spore syringe, print, swab, etc.)?

There is a right and wrong way to do everything. In this scenario, assuming sterile procedure is in place, I have no idea how you could be doing it wrong.

'Was I supposed to make lines with the marijuana before I snorted it, or can I just plug it?'


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InvisibleDoc_T
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Re: Agar & PE Swab Questions... [Re: SoreSpore]
    #16540318 - 07/16/12 04:03 PM (11 years, 7 months ago)

Well you don't need a flowhood to rub spores onto agar.
I tap it like I said, but it would be pretty easy to make a spore syringe too, just rinse the spores into a shotglass and suck them up.


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Invisibleyutaka
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Re: Agar & PE Swab Questions... [Re: Doc_T]
    #16540791 - 07/16/12 05:40 PM (11 years, 7 months ago)

.


Edited by yutaka (01/14/16 03:48 PM)


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InvisiblePhoenix Crash
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Re: Agar & PE Swab Questions... [Re: yutaka]
    #16555664 - 07/19/12 05:34 PM (11 years, 6 months ago)

What if I were to fill a syringe with boiling distilled water and let it cool for an hour or so. Then, using a sterilized shot glass, purge the syringe into the shot glass. Then swirl the end of the swab in the water before sucking the water back into the syringe. If this would work, it would allow the spores time to rehydrate before being squirted onto the agar plate.


--------------------
Why is pot against the law? It wouldn't be because anyone can grow it, and therefore you can't make a profit off it, would it? - Bill Hicks



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"Not all drugs are good, all right? Some of them… are fuckin' great. Just gotta know your way around them, is all."


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InvisibleDoc_T
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Re: Agar & PE Swab Questions... [Re: Phoenix Crash]
    #16555668 - 07/19/12 05:36 PM (11 years, 6 months ago)

Why ruin a swab that has thousands of spare spores on it?


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InvisiblePhoenix Crash
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Re: Agar & PE Swab Questions... [Re: Doc_T]
    #16555691 - 07/19/12 05:41 PM (11 years, 6 months ago)

I've got a handful of swabs, so wasting a few million spores isn't really an issue. Was just wondering if that would be a clean way to rehydrate them and make getting them on agar a little easier.


--------------------
Why is pot against the law? It wouldn't be because anyone can grow it, and therefore you can't make a profit off it, would it? - Bill Hicks



Phoenix Crash LC Tek
DIY Magnetic Stir Bars Tek

"Not all drugs are good, all right? Some of them… are fuckin' great. Just gotta know your way around them, is all."


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InvisibleDoc_T
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Re: Agar & PE Swab Questions... [Re: Phoenix Crash]
    #16555779 - 07/19/12 06:04 PM (11 years, 6 months ago)

It's certainly not any easier than what I said three days ago. :shrug:

Do what you like. They are just spores, you can manage this.


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OfflineWing
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Re: Agar & PE Swab Questions... [Re: Doc_T]
    #16556611 - 07/19/12 08:33 PM (11 years, 6 months ago)

Yea don't over think it.

Honestly I would skip the syringe for now. Spores directly to agar, so you know your working with clean stuff. Then agar to grains. Then make GLC or do G2G.


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