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OfflineAceofShroomz
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Advice on white vs. grey mycelium! PICZ
    #16520819 - 07/12/12 03:43 PM (11 years, 6 months ago)

So here are 2 jars that were inoculated and prepared exactly the same. BRF + Verm. One set I decided to introduce into a colonization chamber with an aquarium heater set in a jar of water for temperature control. After 1-2 days I noticed it added some moisture and water condensation in the inside of the jars so I took them out. After talking with a few people on here I realized that this method was outdated and completely unnecessary. It has been a week+ since I removed the jars from the heating setup and now the mycelium seems to be growing in a very greyish, milky fashion. Unlike the other which is bright white and looking wonderful. Should I toss out the "bad jars". I do not think they are contaminated. But is this mycelium going to grow properly now and most importantly will it fruit properly. Is it in my best interest to learn a lesson and start over? Or have any of you had a similar experience and everything turned out fine.. Any help advice is greatly appreciated! :laugh: I'm a noob but I'm trying so go easy lol. Here's the pics:


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"We are the artists, painting on the blank canvas of Reality." ~Me
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All information & pictures I post are falsified for entertainment purposes only.


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InvisibleDoc_T
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Re: Advice on white vs. grey mycelium! PICZ [Re: AceofShroomz]
    #16520824 - 07/12/12 03:45 PM (11 years, 6 months ago)

you don't think they are contaminated?


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OfflineAceofShroomz
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Re: Advice on white vs. grey mycelium! PICZ [Re: AceofShroomz]
    #16520858 - 07/12/12 03:51 PM (11 years, 6 months ago)

No.. Simply because of what I have read. I thought they just looked like there was too much condensation. There's no other colors besides white. Do they look contaminated to you?


--------------------
"We are the artists, painting on the blank canvas of Reality." ~Me
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All information & pictures I post are falsified for entertainment purposes only.


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OfflineAceofShroomz
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Re: Advice on white vs. grey mycelium! PICZ [Re: AceofShroomz]
    #16520874 - 07/12/12 03:52 PM (11 years, 6 months ago)

Well the jar on the right I would say definitely not. I am just using that as a comparison for what is happening to the other jars. I am really in the dark as to whether they are contaminated or what I should do...


--------------------
"We are the artists, painting on the blank canvas of Reality." ~Me
The Mushroom Speaks

All information & pictures I post are falsified for entertainment purposes only.


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Offlinepsillymathhead
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Re: Advice on white vs. grey mycelium! PICZ [Re: AceofShroomz]
    #16520902 - 07/12/12 03:59 PM (11 years, 6 months ago)

I am very suspicious of the left jar.  Can you smell anything...  A lot of times heater setups will cause bacterial infections, which you can normally smell...


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OfflineAceofShroomz
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Re: Advice on white vs. grey mycelium! PICZ [Re: AceofShroomz]
    #16520936 - 07/12/12 04:04 PM (11 years, 6 months ago)

No I have really gone through all of the "bad jars" and there is no smell and what you are seeing in the picture is the worst of it.  :takingnotes: I'm taking mental notes on all advice and I really appreciate any help...


--------------------
"We are the artists, painting on the blank canvas of Reality." ~Me
The Mushroom Speaks

All information & pictures I post are falsified for entertainment purposes only.


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OfflineAceofShroomz
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Re: Advice on white vs. grey mycelium! PICZ [Re: AceofShroomz]
    #16520945 - 07/12/12 04:05 PM (11 years, 6 months ago)

I'm surprised I can't seem to really find information on any other noobs having the same problem/experience. Which is why I posted this. I always search through the threads to find an answer to my problems, but I just can't really find anything


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"We are the artists, painting on the blank canvas of Reality." ~Me
The Mushroom Speaks

All information & pictures I post are falsified for entertainment purposes only.


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OfflineMateo
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Re: Advice on white vs. grey mycelium! PICZ [Re: psillymathhead]
    #16520966 - 07/12/12 04:08 PM (11 years, 6 months ago)

The grayish jar is contaminated in my oppinion, but im no contam expert and pictures are allways hard to analyze.
I would remove it and empty it in the garden and put a thin layer of soil ontop.
Maybee you get lucky and get some shrooms outside.
Why risk your other jar that seems healthy?


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OfflineAceofShroomz
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Re: Advice on white vs. grey mycelium! PICZ [Re: AceofShroomz]
    #16520983 - 07/12/12 04:12 PM (11 years, 6 months ago)

Yeah I'm uploading a couple more pics but I think that the milky/greyish jars are contaminated or f@cked in one way or another :frown: *cries*


--------------------
"We are the artists, painting on the blank canvas of Reality." ~Me
The Mushroom Speaks

All information & pictures I post are falsified for entertainment purposes only.


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OfflineAceofShroomz
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Re: Advice on white vs. grey mycelium! PICZ [Re: AceofShroomz]
    #16521026 - 07/12/12 04:22 PM (11 years, 6 months ago)

Here's more pics. I'd like a couple more opinions before I throw these jars out and start over from scratch.. Pleassee


--------------------
"We are the artists, painting on the blank canvas of Reality." ~Me
The Mushroom Speaks

All information & pictures I post are falsified for entertainment purposes only.


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OfflinePureHash
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Re: Advice on white vs. grey mycelium! PICZ [Re: AceofShroomz]
    #16521043 - 07/12/12 04:24 PM (11 years, 6 months ago)

The gray one is cobweb.

The white one kinda looks like bacteria. I only say this becasue mycelium doesnt branch out like that. Its hard to tell with thoe picture but here is a picture I have.

Healthy mycelium on the left, and bacteria on the right. Notice the difference?



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OfflinePureHash
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Re: Advice on white vs. grey mycelium! PICZ [Re: PureHash]
    #16521050 - 07/12/12 04:25 PM (11 years, 6 months ago)

There all bunk man, Throw em away.


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Offlinei GrOw StUFF
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Re: Advice on white vs. grey mycelium! PICZ [Re: PureHash]
    #16521059 - 07/12/12 04:27 PM (11 years, 6 months ago)

Quote:

PureHash said:
The gray one is cobweb.

The white one kinda looks like bacteria. I only say this becasue mycelium doesnt branch out like that. Its hard to tell with thoe picture but here is a picture I have.

Healthy mycelium on the left, and bacteria on the right. Notice the difference?






Mycelium branches out all the time.  You don't "see" bacteria, you just "see" the effects of it.


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The mushrooms, which grow only during the season of torrential rains, awaken the forces of creation and produce an experience of spiritual abundance, of an astonishing, inexhaustible constitution of forms that identifies them with fertility and makes them a mediation, a means of communion, of communication between man and the natural world of which they are the metaphysical flesh.


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Offlinei GrOw StUFF
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Re: Advice on white vs. grey mycelium! PICZ [Re: i GrOw StUFF]
    #16521067 - 07/12/12 04:29 PM (11 years, 6 months ago)

and to the OP....the white one looks good in my opinion.  The gray one can be cobweb, it doesn't look too good.


--------------------
The mushrooms, which grow only during the season of torrential rains, awaken the forces of creation and produce an experience of spiritual abundance, of an astonishing, inexhaustible constitution of forms that identifies them with fertility and makes them a mediation, a means of communion, of communication between man and the natural world of which they are the metaphysical flesh.


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OfflineAceofShroomz
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Re: Advice on white vs. grey mycelium! PICZ [Re: i GrOw StUFF]
    #16521083 - 07/12/12 04:32 PM (11 years, 6 months ago)

Thanks I also think the white is good. Compared to everything I've seen it seems fine. Unfortunately all the others will be thrown away. The pictures are taken on my cellphone so they aren't great. Man what a freakin bummer. At least I have a liquid culture that's almost ready to inoculate with. Live and learn. I knew the heater was a horrible idea, was just trying to speed up the process. Patience is a virtue indeed.


--------------------
"We are the artists, painting on the blank canvas of Reality." ~Me
The Mushroom Speaks

All information & pictures I post are falsified for entertainment purposes only.


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Re: Advice on white vs. grey mycelium! PICZ [Re: AceofShroomz]
    #16521093 - 07/12/12 04:34 PM (11 years, 6 months ago)

LC?!?! ARgh!

If so many jars are bad, your LC likely is too.
Proceed with extreme caution as far as that LC goes.
I'd toss it and start over with agar, personally. Agar is cheaper and easier than it looks.


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OfflinePureHash
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Re: Advice on white vs. grey mycelium! PICZ [Re: Doc_T]
    #16521101 - 07/12/12 04:36 PM (11 years, 6 months ago)

^

The white one is only the front of the white one :wink:

All the grey ones are contammmed


Quote:

Doc_T said:
LC?!?! ARgh!

If so many jars are bad, your LC likely is too.
Proceed with extreme caution as far as that LC goes.
I'd toss it and start over with agar, personally. Agar is cheaper and easier than it looks.




:whathesaid:


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Offlinei GrOw StUFF
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Re: Advice on white vs. grey mycelium! PICZ [Re: AceofShroomz]
    #16521102 - 07/12/12 04:36 PM (11 years, 6 months ago)

Quote:

AceofShroomz said:
Thanks I also think the white is good. Compared to everything I've seen it seems fine. Unfortunately all the others will be thrown away. The pictures are taken on my cellphone so they aren't great. Man what a freakin bummer. At least I have a liquid culture that's almost ready to inoculate with. Live and learn. I knew the heater was a horrible idea, was just trying to speed up the process. Patience is a virtue indeed.




Make sure to test the LC out before you go all out on 50 jars.  Heaters are always a bad idea.  Make sure your sterile procedures are up to par too, also make sure your spores are from a good vendor.


--------------------
The mushrooms, which grow only during the season of torrential rains, awaken the forces of creation and produce an experience of spiritual abundance, of an astonishing, inexhaustible constitution of forms that identifies them with fertility and makes them a mediation, a means of communion, of communication between man and the natural world of which they are the metaphysical flesh.


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OfflineAceofShroomz
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Re: Advice on white vs. grey mycelium! PICZ [Re: AceofShroomz]
    #16521115 - 07/12/12 04:38 PM (11 years, 6 months ago)

Actually none of these were inoculated with the LC. This was created afterwards, with different spores, and in a different setup etc. The LC has nothing to do with the initial colonized substrate jars which were colonized with spore syringes. I'm going to inoculate some with the LC this time, some with syringes. I'm just trying different things. Something will work out for Me. I'm not the type to give up, yet I do get frustrated. Such a bummer peoples..


--------------------
"We are the artists, painting on the blank canvas of Reality." ~Me
The Mushroom Speaks

All information & pictures I post are falsified for entertainment purposes only.


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Offlinei GrOw StUFF
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Re: Advice on white vs. grey mycelium! PICZ [Re: Doc_T]
    #16521118 - 07/12/12 04:39 PM (11 years, 6 months ago)

Quote:

Doc_T said:
LC?!?! ARgh!

If so many jars are bad, your LC likely is too.
Proceed with extreme caution as far as that LC goes.
I'd toss it and start over with agar, personally. Agar is cheaper and easier than it looks.




Agar isn't for rookies.  Recommending agar to someone struggling with a syringe and PF-Tek just isn't the best idea.  No offense to the OP.


--------------------
The mushrooms, which grow only during the season of torrential rains, awaken the forces of creation and produce an experience of spiritual abundance, of an astonishing, inexhaustible constitution of forms that identifies them with fertility and makes them a mediation, a means of communion, of communication between man and the natural world of which they are the metaphysical flesh.


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