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BjJiggles
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Re: Advice on white vs. grey mycelium! PICZ [Re: AceofShroomz]
#16522368 - 07/12/12 08:52 PM (11 years, 6 months ago) |
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Yeah I totally agree, mycelium can get super rhizomorphic, look at some agar plates after a few transfers, look at azure myc.... Look at my agar plate in my sig 5th at was one transfer from clone, it just kept gettin more rhizo and agressive after that, is that bacteria on the plate???
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 Alan Rockefeller said:No! Do not feed the type collection of a new species to animals!
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TheEaglesGift
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Registered: 04/10/11
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Re: Advice on white vs. grey mycelium! PICZ [Re: AceofShroomz]
#16523219 - 07/12/12 11:22 PM (11 years, 6 months ago) |
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Quote:
AceofShroomz said: Hmmmm...
To clarify, my comment regarding the bacteria was not directed towards your pictures but rather to the two that PureHash posted.
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AceofShroomz
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Re: Advice on white vs. grey mycelium! PICZ [Re: TheEaglesGift]
#16524881 - 07/13/12 10:59 AM (11 years, 6 months ago) |
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I totally got that Eagles Gift I just thought it was funny because the other guy was preaching and he didn't seem to know as much as he thought. I know either way my jars were turfed! BjJiggles knows what's up LOOOLL. But yeah his pic looks like the mycelium in my left jar. Man this can be a confusing sport, ripe with mystery and debates abound.. Gotta love love love the Shroomz
-------------------- "We are the artists, painting on the blank canvas of Reality." ~Me The Mushroom Speaks
    All information & pictures I post are falsified for entertainment purposes only.
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i GrOw StUFF
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Re: Advice on white vs. grey mycelium! PICZ [Re: AceofShroomz]
#16524911 - 07/13/12 11:08 AM (11 years, 6 months ago) |
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yeah, he started trying to flame me, what a joke. If I don't know what I'm talking about then I keep my trap shut.
-------------------- The mushrooms, which grow only during the season of torrential rains, awaken the forces of creation and produce an experience of spiritual abundance, of an astonishing, inexhaustible constitution of forms that identifies them with fertility and makes them a mediation, a means of communion, of communication between man and the natural world of which they are the metaphysical flesh.
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BjJiggles
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Re: Advice on white vs. grey mycelium! PICZ [Re: i GrOw StUFF]
#16525000 - 07/13/12 11:25 AM (11 years, 6 months ago) |
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Same here, not the first time he's been an ass right off the bat... Idk y ya gotta be like that... I guess ego plays a part, which is fine I guess, just sux when ur wrong n u look like an ass, lol...
Anyway, sry bot ur jars OP... Good luck in the future..
--------------------
 Alan Rockefeller said:No! Do not feed the type collection of a new species to animals!
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AceofShroomz
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Re: Advice on white vs. grey mycelium! PICZ [Re: AceofShroomz]
#16525324 - 07/13/12 12:29 PM (11 years, 6 months ago) |
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Thanks man, ya know at least I won't make the same mistakes again. I'll make NEW ones! But I'm excited, was up until 3 am sterilizing the new substrate jars. Got 18 of em! I'm preparing for inoculation right meow. BLAMM JARZ:
-------------------- "We are the artists, painting on the blank canvas of Reality." ~Me The Mushroom Speaks
    All information & pictures I post are falsified for entertainment purposes only.
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AceofShroomz
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Re: Advice on white vs. grey mycelium! PICZ [Re: AceofShroomz]
#16525350 - 07/13/12 12:32 PM (11 years, 6 months ago) |
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And yeah it always cracks me up when people preach about something they aren't so knowledgeable about. I read a lot about rhizomorphic growth (since it was brought up) and the difference between contaminated "cobwebs". One of my jars was cobwebbed and gross. But the other looked typical rhizomorphic. Sadly I only have 2 of those jars. But oh well. They look pretty good so hopefully I'll get some fruits out of em. More just concentrating on the next batch though. Testing 2 different LC's so I'm excited to see how those inoculate and if they are contaminated or not..
-------------------- "We are the artists, painting on the blank canvas of Reality." ~Me The Mushroom Speaks
    All information & pictures I post are falsified for entertainment purposes only.
Edited by AceofShroomz (07/13/12 12:33 PM)
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TheEaglesGift
The Nagual


Registered: 04/10/11
Posts: 10,554
Loc: Ixtlan, Mexico
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Re: Advice on white vs. grey mycelium! PICZ [Re: PureHash]
#16525572 - 07/13/12 01:14 PM (11 years, 6 months ago) |
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Quote:
PureHash said:
Quote:
i GrOw StUFF said:
Quote:
PureHash said: The gray one is cobweb.
The white one kinda looks like bacteria. I only say this becasue mycelium doesnt branch out like that. Its hard to tell with thoe picture but here is a picture I have.
Healthy mycelium on the left, and bacteria on the right. Notice the difference?

Mycelium branches out all the time. You don't "see" bacteria, you just "see" the effects of it.
Don't blurt out incorrect information. Mycelium does not branch out, it may follow different pats, but each "branch is made of thousands of strands. His pictures, they look solid.
And yes. You CAN see certan types of bacteria. There are thousands of different types of it. I've even been mystaken myself and tried to fruit bacteria cakes. Witch obviously did not happen.... The cake turned orange within 24 hours.
Don't regurgitate false info.
Two pictures of mycelium? One being very rhizomorphic? Where is the bacteria? I'm not seeing it.
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FruitOfLife
Professional Package Handler


Registered: 05/21/12
Posts: 4,832
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Re: Advice on white vs. grey mycelium! PICZ [Re: AceofShroomz]
#16525581 - 07/13/12 01:17 PM (11 years, 6 months ago) |
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I just got my first signs of mycelium today and they are starting to look like op's grey jars. The spots right now are still small so its too hard to get a good picture of it, but 4 jars have greyish white spots and one jar has good white mycelium.
Will it be noticeable to tell the difference between mycelium and cobweb once the jars are 50% or so?
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BjJiggles
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Re: Advice on white vs. grey mycelium! PICZ [Re: FruitOfLife]
#16525813 - 07/13/12 02:08 PM (11 years, 6 months ago) |
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Cobweb will grow a lot faster than mycelium and have a noticeable gray tint... Also appears really fluffy and cottony, not rhizo at all..
--------------------
 Alan Rockefeller said:No! Do not feed the type collection of a new species to animals!
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TheEaglesGift
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Registered: 04/10/11
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Loc: Ixtlan, Mexico
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Re: Advice on white vs. grey mycelium! PICZ [Re: FruitOfLife]
#16525900 - 07/13/12 02:28 PM (11 years, 6 months ago) |
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Quote:
Drewsifer69 said: I just got my first signs of mycelium today and they are starting to look like op's grey jars. The spots right now are still small so its too hard to get a good picture of it, but 4 jars have greyish white spots and one jar has good white mycelium.
Will it be noticeable to tell the difference between mycelium and cobweb once the jars are 50% or so?
When mushroom mycelium first starts out it can appear grey. As time goes by it will become more apparent whether you have mushroom or mold mycelium.
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AceofShroomz
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Re: Advice on white vs. grey mycelium! PICZ [Re: AceofShroomz]
#16525925 - 07/13/12 02:33 PM (11 years, 6 months ago) |
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Yes I noticed the cobweb effect progressed MUCH faster than the pure white mycelium growing in a rhizomorphic way. I just finished inoculating the 18 new jars! 8 Ecuadorian, 8 Palensque (sp?), and 1 jar from 2 different LC's. Picture of my Wonderful Closet of MAGICK:
-------------------- "We are the artists, painting on the blank canvas of Reality." ~Me The Mushroom Speaks
    All information & pictures I post are falsified for entertainment purposes only.
Edited by AceofShroomz (07/13/12 02:33 PM)
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Mateo
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Re: Advice on white vs. grey mycelium! PICZ [Re: BjJiggles]
#16525993 - 07/13/12 02:42 PM (11 years, 6 months ago) |
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If not all of your jars contaminate and you used the same spore syringe then it might be your sterilization teknique that is the culprit. Are you PC'ing your jars for atleast an hour? Are you really clean, use mouth protection & alcohole washed gloves and follow the standard procedures when inoculating your jars as flame sterilizing the needle, whipe everything down with 70% isopropanol, use a glovebox/SAB?
I feel something other than a bad sporesyringe is causing this.
How is the air in the room you use when inoculating the jars, is it totaly still, no drafts, AC of fans going? I think you must read up on the details of sterile work. Buy P.Stamets 2 good books on mushroomgrowing or read up on the internet. It's in the details you know, everything is important.
Good luck and don't give up. Once you get the hang of it, its easy.
/Mateo
-------------------- A wise rat has many holes
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AceofShroomz
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Re: Advice on white vs. grey mycelium! PICZ [Re: Mateo]
#16526034 - 07/13/12 02:50 PM (11 years, 6 months ago) |
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Quote:
Mateo said: If not all of your jars contaminate and you used the same spore syringe then it might be your sterilization teknique that is the culprit. Are you PC'ing your jars for atleast an hour? Are you really clean, use mouth protection & alcohole washed gloves and follow the standard procedures when inoculating your jars as flame sterilizing the needle, whipe everything down with 70% isopropanol, use a glovebox/SAB?
I feel something other than a bad sporesyringe is causing this.
How is the air in the room you use when inoculating the jars, is it totaly still, no drafts, AC of fans going? I think you must read up on the details of sterile work. Buy P.Stamets 2 good books on mushroomgrowing or read up on the internet. It's in the details you know, everything is important.
Good luck and don't give up. Once you get the hang of it, its easy.
/Mateo
I am confident the contamination was from the heating element. As I stated it introduced a crazy amount of moisture and when I took them to the graveyard their cores were nearly soaked. I feel this would contaminate any jars and it was a silly idea that I was trying. I just wanted faster growth. I have read and follow ALL the sterilization techniques. I turn off the ac and fans and close the windows 2 hours prior to any work. I use glove and 70% isopropyl alcohol. There's no shortage of research here. The only difference with what you've stated is that I use steam sterilization in the timeframe of about 90 minutes. I just can't afford a pressure cooker at the moment. I basically used this video to a T: .. Unless you're referring to someone else I'm not sure where you got the idea that I think I have bad spore syringes. The contamination IMO came from elsewhere, even though that is possible.
-------------------- "We are the artists, painting on the blank canvas of Reality." ~Me The Mushroom Speaks
    All information & pictures I post are falsified for entertainment purposes only.
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AceofShroomz
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Re: Advice on white vs. grey mycelium! PICZ [Re: AceofShroomz]
#16526054 - 07/13/12 02:52 PM (11 years, 6 months ago) |
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If you look in my picture I just posted on the left the 2 jars that weren't exposed to the heating element/moisture are doing just fine. And they were inoculated in the same way and at the same time as the other dozen that I had to throw away.
-------------------- "We are the artists, painting on the blank canvas of Reality." ~Me The Mushroom Speaks
    All information & pictures I post are falsified for entertainment purposes only.
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AceofShroomz
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Re: Advice on white vs. grey mycelium! PICZ [Re: AceofShroomz]
#16526136 - 07/13/12 03:06 PM (11 years, 6 months ago) |
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And I forgot last time I did not use a glove box! Sadly. Even though it seemed it wasn't a problem, this time I used the "oven technique". It seems rather lame honestly but I think it will work fine. You turn the oven on low and inoculate the jars on the oven rack. Basically the rising hot air keeps airborne contaminates and bacteria etc. from falling onto the jars during inoculation. Similar to the glovebox, except instead of still air, you have constantly rising air. I'm sure it's very debatable whether this will work. I'll be finding out shortly lol. I know, I take a lot of risks.. I run my Life that way and it works out fine. Knowledge & Wisdom are the bi-product of "mistakes"..
-------------------- "We are the artists, painting on the blank canvas of Reality." ~Me The Mushroom Speaks
    All information & pictures I post are falsified for entertainment purposes only.
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Mateo
High on LIFE!



Registered: 06/24/11
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Re: Advice on white vs. grey mycelium! PICZ [Re: AceofShroomz]
#16526158 - 07/13/12 03:10 PM (11 years, 6 months ago) |
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Well, then you probably have found you contamination source. I didn't read all the thread, thats why i said the syringes might have been bad. Sorry. Your next tries will hopfully look much better. You seem to have read up and have knowledge of what you are doing. Don't take any more shortcuts for faster results though. Start to do by the book and then when sucessful you can try experiments if you think they will turn out good. Then if your experiments turn bad you can always go back to the safe method.
Good luck...
/Mateo
-------------------- A wise rat has many holes
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AceofShroomz
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Re: Advice on white vs. grey mycelium! PICZ [Re: Mateo]
#16526191 - 07/13/12 03:16 PM (11 years, 6 months ago) |
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Thanks for the support man! I love this site as opposed to other Mycology sites. Such a friendly and knowledgable group. I haven't even fruited any shroomz yet and I know A LOT mostly due to this site lol. I'll keep posting pictures of my adventures and who knows; Maybe soon I'll have enough creative techniques to do my own write-up(s). That's what I'm seeing here, there's really a lot of room for creative growth as far as technique. I can't help but feel there's still a lot to be discovered as far as Cultivation is concerned. If you look at the old techniques (such as my buddy gave me this book "Psilocybin" from the 80's) it's pretty laughable compared to what you guys are doing. STILL good info in there, don't get me wrong. But the progress being made in this field is wonderful, and seems mostly to be by people like you and Me.. Who are just trying new things. You guys all Rock thanks for Everything!!    
-------------------- "We are the artists, painting on the blank canvas of Reality." ~Me The Mushroom Speaks
    All information & pictures I post are falsified for entertainment purposes only.
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Mateo
High on LIFE!



Registered: 06/24/11
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Re: Advice on white vs. grey mycelium! PICZ [Re: Mateo]
#16526233 - 07/13/12 03:23 PM (11 years, 6 months ago) |
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Also, might i sugest you start looking into how to work with agar. Agar work is fun. Agar work can help you get much better results. Agar is easy. It's never too soon to start with agar.
You can doubble your result if doing a strain isollation and find a good producing isolate. And you will get a ton of experience of how the mycelium work in different situations and for example you will be able to save a contaminated sporeprint.
Just a suggestion...
-------------------- A wise rat has many holes
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AceofShroomz
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Re: Advice on white vs. grey mycelium! PICZ [Re: AceofShroomz]
#16526263 - 07/13/12 03:29 PM (11 years, 6 months ago) |
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Thanks that's actually a really good suggestion and I haven't researched about Agar work AT ALL yet. It seems really intimidating, honestly. And with all of the other research I've had to do (weeks in front of the computer it seems) just to get this going, I was planning on waiting. Do you have any advice on a good read to get me started? I've seen it mentioned of course, but I really have no idea how it works, or the benefits therein. I wouldn't even know where to buy agar lol. But I'm really interested and hungry for the information..... ;D
-------------------- "We are the artists, painting on the blank canvas of Reality." ~Me The Mushroom Speaks
    All information & pictures I post are falsified for entertainment purposes only.
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