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Offlinei GrOw StUFF
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Re: Advice on white vs. grey mycelium! PICZ [Re: AceofShroomz]
    #16521128 - 07/12/12 04:40 PM (11 years, 6 months ago)

Quote:

AceofShroomz said:
Actually none of these were inoculated with the LC. This was created afterwards, with different spores, and in a different setup etc. The LC has nothing to do with the initial colonized substrate jars which were colonized with spore syringes. I'm going to inoculate some with the LC this time, some with syringes. I'm just trying different things. Something will work out for Me. I'm not the type to give up, yet I do get frustrated. Such a bummer peoples..




LC's are awesome when they come out clean.  It's all a learning process, whatever you get to work stick with it.


--------------------
The mushrooms, which grow only during the season of torrential rains, awaken the forces of creation and produce an experience of spiritual abundance, of an astonishing, inexhaustible constitution of forms that identifies them with fertility and makes them a mediation, a means of communion, of communication between man and the natural world of which they are the metaphysical flesh.


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InvisibleDoc_T
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Re: Advice on white vs. grey mycelium! PICZ [Re: i GrOw StUFF]
    #16521154 - 07/12/12 04:45 PM (11 years, 6 months ago)

^^^ "IF" they come out clean.

But even then it's still just multispore for most growers.
Relatively few clone or isolate and then make LC from that culture.


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Re: Advice on white vs. grey mycelium! PICZ [Re: i GrOw StUFF]
    #16521164 - 07/12/12 04:48 PM (11 years, 6 months ago)

Quote:

i GrOw StUFF said:
Agar isn't for rookies.




I completely disagree.
Anybody with a glovebox and a spore syringe is ready to begin agar work.

...

when I studied martial arts, some of the best moves were white belt material.
It took a year or more of practice to be able to pull off some of this stuff, but other moves worked the first time.
It's like that with agar work, you'll be learning more until you die- but start right now, you'll be surprised how well it works.


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OfflineAceofShroomz
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Re: Advice on white vs. grey mycelium! PICZ [Re: Doc_T]
    #16521193 - 07/12/12 04:57 PM (11 years, 6 months ago)

http://mushroompalace.com/grow-your-own/liquid-culture
This is the website I used to make the liquid culture. But yeah I'll probably skip on that for now and just try again with the classic pf-tek. I really think that the whole heating element is what caused this. It introduced a lot of extra moisture into the jars very quickly, which I'm sure carried some sort of contaminates.


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"We are the artists, painting on the blank canvas of Reality." ~Me
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InvisibleDoc_T
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Re: Advice on white vs. grey mycelium! PICZ [Re: AceofShroomz]
    #16521203 - 07/12/12 04:58 PM (11 years, 6 months ago)

Doesn't matter how you make it if you shoot spores from a dirty syringe.
Al the sterile technique in the world can't overcome dirty inoculant.

Meanwhile agar work is cheaper and easier than it looks by far.


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Offlinei GrOw StUFF
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Re: Advice on white vs. grey mycelium! PICZ [Re: AceofShroomz]
    #16521219 - 07/12/12 05:02 PM (11 years, 6 months ago)

Quote:

AceofShroomz said:
http://mushroompalace.com/grow-your-own/liquid-culture
This is the website I used to make the liquid culture. But yeah I'll probably skip on that for now and just try again with the classic pf-tek. I really think that the whole heating element is what caused this. It introduced a lot of extra moisture into the jars very quickly, which I'm sure carried some sort of contaminates.




Heating the jars caused the moisture to invade the dry verm layer which invited the contams.  Where did you get the syringes?


--------------------
The mushrooms, which grow only during the season of torrential rains, awaken the forces of creation and produce an experience of spiritual abundance, of an astonishing, inexhaustible constitution of forms that identifies them with fertility and makes them a mediation, a means of communion, of communication between man and the natural world of which they are the metaphysical flesh.


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OfflineAceofShroomz
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Re: Advice on white vs. grey mycelium! PICZ [Re: AceofShroomz]
    #16521221 - 07/12/12 05:02 PM (11 years, 6 months ago)

I'll post some pics of the LC if you guys want. It's rather soonish I don't think you can see the growth in pictures yet. When you agitate the jars you can see fluffy stuff floating around that looks consistent with pics I have seen. The water isn't getting cloudy or anything. Who knows. For the moment I'm lightweight devastated. I'll be prepping and getting everything ready for another inoculation procedure tonight. I've been wanting to do this for a long time and I'm not giving up. I'm disappointed that as soon as I used the heating technique a lot of people told me that is a horrible idea. The fact that I was able to find a lot of people recommending it on write-ups/walk-throughs (not necessarily on this site) is totally uncoolish.


--------------------
"We are the artists, painting on the blank canvas of Reality." ~Me
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All information & pictures I post are falsified for entertainment purposes only.


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OfflineAceofShroomz
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Re: Advice on white vs. grey mycelium! PICZ [Re: AceofShroomz]
    #16521227 - 07/12/12 05:03 PM (11 years, 6 months ago)

.


--------------------
"We are the artists, painting on the blank canvas of Reality." ~Me
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Edited by Prisoner#1 (07/12/12 06:11 PM)


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Offlinei GrOw StUFF
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Re: Advice on white vs. grey mycelium! PICZ [Re: AceofShroomz]
    #16521233 - 07/12/12 05:04 PM (11 years, 6 months ago)

Quote:

AceofShroomz said:
I'll post some pics of the LC if you guys want. It's rather soonish I don't think you can see the growth in pictures yet. When you agitate the jars you can see fluffy stuff floating around that looks consistent with pics I have seen. The water isn't getting cloudy or anything. Who knows. For the moment I'm lightweight devastated. I'll be prepping and getting everything ready for another inoculation procedure tonight. I've been wanting to do this for a long time and I'm not giving up. I'm disappointed that as soon as I used the heating technique a lot of people told me that is a horrible idea. The fact that I was able to find a lot of people recommending it on write-ups/walk-throughs (not necessarily on this site) is totally uncoolish.




you can't tell if an LC is clean until you test it.  Even with pics...


--------------------
The mushrooms, which grow only during the season of torrential rains, awaken the forces of creation and produce an experience of spiritual abundance, of an astonishing, inexhaustible constitution of forms that identifies them with fertility and makes them a mediation, a means of communion, of communication between man and the natural world of which they are the metaphysical flesh.


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OfflineAceofShroomz
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Re: Advice on white vs. grey mycelium! PICZ [Re: i GrOw StUFF]
    #16521238 - 07/12/12 05:05 PM (11 years, 6 months ago)

Quote:

i GrOw StUFF said:
Quote:

AceofShroomz said:
http://mushroompalace.com/grow-your-own/liquid-culture
This is the website I used to make the liquid culture. But yeah I'll probably skip on that for now and just try again with the classic pf-tek. I really think that the whole heating element is what caused this. It introduced a lot of extra moisture into the jars very quickly, which I'm sure carried some sort of contaminates.




Heating the jars caused the moisture to invade the dry verm layer which invited the contams.  Where did you get the syringes?




I'm nearly positive that's what happened^ Because I didn't introduce the heating element until about a week into colonization, and everything looked good then. Ever since I tried the heating, it just kept getting worse, and worse, and WORSE. Until today I've thrown them all out. I think this next time will go much more smoothly. I've learned a lot so far.


--------------------
"We are the artists, painting on the blank canvas of Reality." ~Me
The Mushroom Speaks

All information & pictures I post are falsified for entertainment purposes only.


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OfflineAceofShroomz
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Re: Advice on white vs. grey mycelium! PICZ [Re: i GrOw StUFF]
    #16521256 - 07/12/12 05:09 PM (11 years, 6 months ago)

Quote:

i GrOw StUFF said:

you can't tell if an LC is clean until you test it.  Even with pics...




Well I will be doing approximately 20 jars tonight. I will be trying out the LC on a few of them just to see what happens (since I have 3 LC jars). For the other 17 I'm going to just follow the directions to a T and not try and crazy or complex methods. They will be inoculated properly, and left alone and I WILL NOT eff with them this time lol. Trying to cheat the clock or anything. Tried to be creative, but I don't think I'm ready for that yet.. Obviously lol


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"We are the artists, painting on the blank canvas of Reality." ~Me
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All information & pictures I post are falsified for entertainment purposes only.


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OfflineProject
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Re: Advice on white vs. grey mycelium! PICZ [Re: Doc_T]
    #16521415 - 07/12/12 05:55 PM (11 years, 6 months ago)

Quote:

Doc_T said:
^^^ "IF" they come out clean.

But even then it's still just multispore for most growers.
Relatively few clone or isolate and then make LC from that culture.




:awetongue:


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Offlineya der eh
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Re: Advice on white vs. grey mycelium! PICZ [Re: AceofShroomz]
    #16521432 - 07/12/12 05:59 PM (11 years, 6 months ago)

I wouldn't post the name of any company or person who sent anything to commiefornia


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Re: Advice on white vs. grey mycelium! PICZ [Re: psillymathhead]
    #16521476 - 07/12/12 06:09 PM (11 years, 6 months ago)

Quote:

psillymathhead said:
I am very suspicious of the left jar.  Can you smell anything...  A lot of times heater setups will cause bacterial infections, which you can normally smell...




I see no bacteria. That left jar is clearly a mold of some kind.


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OfflinePureHash
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Re: Advice on white vs. grey mycelium! PICZ [Re: i GrOw StUFF]
    #16521489 - 07/12/12 06:12 PM (11 years, 6 months ago)

Quote:

i GrOw StUFF said:
Quote:

PureHash said:
The gray one is cobweb.

The white one kinda looks like bacteria. I only say this becasue mycelium doesnt branch out like that. Its hard to tell with thoe picture but here is a picture I have.

Healthy mycelium on the left, and bacteria on the right. Notice the difference?







Mycelium branches out all the time.  You don't "see" bacteria, you just "see" the effects of it.



Don't blurt out incorrect information. Mycelium does not branch out, it may follow different pats, but each "branch is made of thousands of strands. His pictures, they look solid.

And yes. You CAN see certan types of bacteria. There are thousands of different types of it. I've even been mystaken myself and tried to fruit bacteria cakes. Witch obviously did not happen.... The cake turned orange within 24 hours.

Don't regurgitate false info.


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OfflineAceofShroomz
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Re: Advice on white vs. grey mycelium! PICZ [Re: PureHash]
    #16521547 - 07/12/12 06:26 PM (11 years, 6 months ago)

Thanks everyone. I'll be posting some pics of the next operation once it warrants it. Hopefully everything goes smoothly. The aquarium heater has been returned (get that ish out of my house lol). I did keep the 2 jars with purely white mycelium that weren't exposed to the heating element. It's not really worth throwing them away considering I have lots more jars to play with. I'll probably keep them and use the D&R tek, and fruit them in some shoddy 2 liter coke bottle with perlite at the bottom like I've seen for the "poor man's terrarium". I don't really have any hope for them (seeing as it's only 2 jars anyways) but I'm interested to see what happens with them. When I tossed the substrate from the other jars the core was SO wet. The amount of moisture that the heating element introduced is simply absurd. What a horrible technique IMO.


--------------------
"We are the artists, painting on the blank canvas of Reality." ~Me
The Mushroom Speaks

All information & pictures I post are falsified for entertainment purposes only.


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Offlinei GrOw StUFF
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Re: Advice on white vs. grey mycelium! PICZ [Re: PureHash]
    #16521865 - 07/12/12 07:25 PM (11 years, 6 months ago)

Quote:

PureHash said:
Quote:

i GrOw StUFF said:
Quote:

PureHash said:
The gray one is cobweb.

The white one kinda looks like bacteria. I only say this becasue mycelium doesnt branch out like that. Its hard to tell with thoe picture but here is a picture I have.

Healthy mycelium on the left, and bacteria on the right. Notice the difference?







Mycelium branches out all the time.  You don't "see" bacteria, you just "see" the effects of it.



Don't blurt out incorrect information. Mycelium does not branch out, it may follow different pats, but each "branch is made of thousands of strands. His pictures, they look solid.

And yes. You CAN see certan types of bacteria. There are thousands of different types of it. I've even been mystaken myself and tried to fruit bacteria cakes. Witch obviously did not happen.... The cake turned orange within 24 hours.

Don't regurgitate false info.




No idea where you're coming from? When I say "branching out" I'm referring to rhizomorphic growth which is a sign of healthy mycelium.    When I've seen bacteria set in within a jar it will eventually turn the grain or brf/verm into a mushy type thing but no crazy colors, and the myc forms a border around the contam.......What false info have I regurgitated?  I'm interested...


--------------------
The mushrooms, which grow only during the season of torrential rains, awaken the forces of creation and produce an experience of spiritual abundance, of an astonishing, inexhaustible constitution of forms that identifies them with fertility and makes them a mediation, a means of communion, of communication between man and the natural world of which they are the metaphysical flesh.


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Offlinei GrOw StUFF
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Re: Advice on white vs. grey mycelium! PICZ [Re: i GrOw StUFF]
    #16521914 - 07/12/12 07:34 PM (11 years, 6 months ago)

and when bacteria infects the mycelium it kinda has a weird white look to it....like it's soupy or ice creamish....


--------------------
The mushrooms, which grow only during the season of torrential rains, awaken the forces of creation and produce an experience of spiritual abundance, of an astonishing, inexhaustible constitution of forms that identifies them with fertility and makes them a mediation, a means of communion, of communication between man and the natural world of which they are the metaphysical flesh.


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Re: Advice on white vs. grey mycelium! PICZ [Re: AceofShroomz]
    #16522027 - 07/12/12 07:51 PM (11 years, 6 months ago)

PureHash, both of those pictures that you posted look like cubensis mycelium to me. I don't see any bacteria in the right picture.


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OfflineAceofShroomz
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Re: Advice on white vs. grey mycelium! PICZ [Re: AceofShroomz]
    #16522335 - 07/12/12 08:44 PM (11 years, 6 months ago)

Hmmmm...


--------------------
"We are the artists, painting on the blank canvas of Reality." ~Me
The Mushroom Speaks

All information & pictures I post are falsified for entertainment purposes only.


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