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Mateo
High on LIFE!



Registered: 06/24/11
Posts: 2,689
Loc: North Europe
Last seen: 3 years, 3 months
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Re: Advice on white vs. grey mycelium! PICZ [Re: psillymathhead]
#16520966 - 07/12/12 04:08 PM (11 years, 6 months ago) |
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The grayish jar is contaminated in my oppinion, but im no contam expert and pictures are allways hard to analyze. I would remove it and empty it in the garden and put a thin layer of soil ontop. Maybee you get lucky and get some shrooms outside. Why risk your other jar that seems healthy?
-------------------- A wise rat has many holes
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Mateo
High on LIFE!



Registered: 06/24/11
Posts: 2,689
Loc: North Europe
Last seen: 3 years, 3 months
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Re: Advice on white vs. grey mycelium! PICZ [Re: BjJiggles]
#16525993 - 07/13/12 02:42 PM (11 years, 6 months ago) |
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If not all of your jars contaminate and you used the same spore syringe then it might be your sterilization teknique that is the culprit. Are you PC'ing your jars for atleast an hour? Are you really clean, use mouth protection & alcohole washed gloves and follow the standard procedures when inoculating your jars as flame sterilizing the needle, whipe everything down with 70% isopropanol, use a glovebox/SAB?
I feel something other than a bad sporesyringe is causing this.
How is the air in the room you use when inoculating the jars, is it totaly still, no drafts, AC of fans going? I think you must read up on the details of sterile work. Buy P.Stamets 2 good books on mushroomgrowing or read up on the internet. It's in the details you know, everything is important.
Good luck and don't give up. Once you get the hang of it, its easy.
/Mateo
-------------------- A wise rat has many holes
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Mateo
High on LIFE!



Registered: 06/24/11
Posts: 2,689
Loc: North Europe
Last seen: 3 years, 3 months
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Re: Advice on white vs. grey mycelium! PICZ [Re: AceofShroomz]
#16526158 - 07/13/12 03:10 PM (11 years, 6 months ago) |
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Well, then you probably have found you contamination source. I didn't read all the thread, thats why i said the syringes might have been bad. Sorry. Your next tries will hopfully look much better. You seem to have read up and have knowledge of what you are doing. Don't take any more shortcuts for faster results though. Start to do by the book and then when sucessful you can try experiments if you think they will turn out good. Then if your experiments turn bad you can always go back to the safe method.
Good luck...
/Mateo
-------------------- A wise rat has many holes
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Mateo
High on LIFE!



Registered: 06/24/11
Posts: 2,689
Loc: North Europe
Last seen: 3 years, 3 months
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Re: Advice on white vs. grey mycelium! PICZ [Re: Mateo]
#16526233 - 07/13/12 03:23 PM (11 years, 6 months ago) |
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Also, might i sugest you start looking into how to work with agar. Agar work is fun. Agar work can help you get much better results. Agar is easy. It's never too soon to start with agar.
You can doubble your result if doing a strain isollation and find a good producing isolate. And you will get a ton of experience of how the mycelium work in different situations and for example you will be able to save a contaminated sporeprint.
Just a suggestion...
-------------------- A wise rat has many holes
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Mateo
High on LIFE!



Registered: 06/24/11
Posts: 2,689
Loc: North Europe
Last seen: 3 years, 3 months
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Re: Advice on white vs. grey mycelium! PICZ [Re: FruitOfLife]
#16526934 - 07/13/12 05:50 PM (11 years, 6 months ago) |
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If you want to read by book P.Stamets books "the mushroom cultivator" and "Growing gormet and medicinal mushrooms" are super good read and goes into depth in every thing. If you want to read online there are very much info in this forum if you search. Just search for agar teks and you find plenty. Its quite intesting to see how a small spore or mycelim piece or a piece of a mushroom start to grow on agar and how it develops with time. Then if you cut out good lookin pieces of the agar dish and put on new agar it developes further. You can pick the most agressive colonizating mycelium and put into new dishes and soon you have a single strain super variety. This can be a super fruiter or it cant fruit at all, but this is how very super producing isolates is discovered. If you find one it can be producing huge yields for a long time for you so its definatly worth it. Also almost all mycelium of cubes will fruit as its a very forgiving mushroom strain to work with. And most inportant you learn alot while doing it.
-------------------- A wise rat has many holes
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