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OfflineOysterFace
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Is it TRUE?? Peroxide as replacement for filter disc in LC??
    #16511789 - 07/10/12 09:23 PM (11 years, 7 months ago)

i was recently reading the baby bottle LC tek in the general cultivation section of shroomery and came across an interesting idea.  here is the link to the page.  if you scroll down to tictoc's post he raises an interesting point to a dilemma that LC presents. 

the baby bottle tek is great, save for the fact that it does not use any method to allow for gas exchange(as far as i know the only method would be to install some kind of filter disc, correct?, and this opens one up to contamination via a wet filter disc if not careful).  this is not such a big deal as the author of the post recommends the bottles be refrigerated after mycelium appears in order to stall its growth.  so, the air left within the bottle after sterilization appears to be sufficient for this rudementary purpose.

Tictoc, however, states that by injecting a small amount of peroxide into the bottle is sufficient for providing the mycelium with extra oxygen.  If im not mistaken,  peroxide does degrade into water and oxygen. correct?  and this seems like a good idea that would prevent contamination and provide a little extra oxygen to the bottled up mycelium.  Does anyone have any ideas about this?  How many times do you suppose peroxide could be added to provide oxygen, if this indeed is a viable alternative to providing a filter disc for FAE, before mycelial growth is hindered or impaired?


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InvisibleDoc_T
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Re: Is it TRUE?? Peroxide as replacement for filter disc in LC?? [Re: OysterFace]
    #16511834 - 07/10/12 09:33 PM (11 years, 7 months ago)

LC sucks, man. Get into agar work.


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OfflineMaverick
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Re: Is it TRUE?? Peroxide as replacement for filter disc in LC?? [Re: Doc_T]
    #16511846 - 07/10/12 09:36 PM (11 years, 7 months ago)

Quote:

Doc_T said:
LC sucks, man. Get into agar work.




I like LCs, so long as you start with Agar first...

Ever try placing a wedge of agar in a grain jar and watching how long it takes to colonize?

LCs are fine, so long as you start with agar, transfer an agar wedge to the LC.  Your contam rate should be nill or close to nill. I haven't had a contaminated LC in YEARS.  But I don't start with spores in LC.

LC is just a method to expand a culture, agar is a way to isolate and find new substrains, isolate away from contams, etc.


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OfflineOysterFace
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Re: Is it TRUE?? Peroxide as replacement for filter disc in LC?? [Re: Maverick]
    #16513120 - 07/11/12 06:20 AM (11 years, 7 months ago)

what type of container do you use for your liquid culture?


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OfflineRogerRabbitM
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Re: Is it TRUE?? Peroxide as replacement for filter disc in LC?? [Re: Maverick]
    #16513702 - 07/11/12 10:20 AM (11 years, 7 months ago)

Quote:

Maverick said:


Ever try placing a wedge of agar in a grain jar and watching how long it takes to colonize?






Yes.  I place an agar wedge on the top of a large filter patch bag containing 6 quarts of rye grain and it's fully colonized in two weeks.  LC adds an extra step, several extra vectors of contamination, and it's slower.
RR


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OfflineOysterFace
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Re: Is it TRUE?? Peroxide as replacement for filter disc in LC?? [Re: RogerRabbit]
    #16516196 - 07/11/12 07:41 PM (11 years, 7 months ago)

does that mean that in your opinion RR  one might be better off not bothering with LC?  And if one did use LC, how necesary is it to give it gas exchange if somone is  just trying to germinate some weak spore water and then inoculate some grains?


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OfflineMaverick
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Re: Is it TRUE?? Peroxide as replacement for filter disc in LC?? [Re: RogerRabbit]
    #16516683 - 07/11/12 09:06 PM (11 years, 7 months ago)

Quote:

RogerRabbit said:
Quote:

Maverick said:


Ever try placing a wedge of agar in a grain jar and watching how long it takes to colonize?






Yes.  I place an agar wedge on the top of a large filter patch bag containing 6 quarts of rye grain and it's fully colonized in two weeks.  LC adds an extra step, several extra vectors of contamination, and it's slower.
RR




Really depends on how good you are with your sterile technique.  Are you saying that opening a jar of grain is less of a contam vector than opening an LC?  They're the same.  That's a false sense of security if you're saying that.


I know you have your biased opinions on LCs, as I've read your posts for years, but there are plenty of us, such as the mods and admins of the live chat, who know what we're saying and doing as well.


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OfflineOysterFace
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Re: Is it TRUE?? Peroxide as replacement for filter disc in LC?? [Re: Maverick]
    #16516980 - 07/11/12 09:53 PM (11 years, 7 months ago)

sooo, can anyone actually answer the subject matter for which this post was written?


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OfflineMaverick
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Re: Is it TRUE?? Peroxide as replacement for filter disc in LC?? [Re: OysterFace]
    #16516985 - 07/11/12 09:54 PM (11 years, 7 months ago)

I wouldn't be adding it to my LCs, theoretically it would decay during PCing, but it's not a replacement for a filter patch.


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OfflineOysterFace
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Re: Is it TRUE?? Peroxide as replacement for filter disc in LC?? [Re: Maverick]
    #16517029 - 07/11/12 10:02 PM (11 years, 7 months ago)

the peroxide would be added afterwards. after the mycelium has grown and need more oxygen to continue.

Can LC be done without a filterpatch(or anything to provide gas exchange)??

some of the teks on this site dont use them so im gonna say yes it CAN be done, but is it advisable. does it pose any serious drawback?


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OfflineMaverick
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Re: Is it TRUE?? Peroxide as replacement for filter disc in LC?? [Re: OysterFace]
    #16517047 - 07/11/12 10:05 PM (11 years, 7 months ago)

Adding it afterwards would not be recommended, you would most likely harm the mycelium, as mycelium in an LC is fairly weak.  You don't even really want to use h2o2 anyway except in the situations of cobweb on substrates, in which case you should drastically increase FAE more than use h2o2.

Don't use h2o2.


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OfflineOysterFace
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Re: Is it TRUE?? Peroxide as replacement for filter disc in LC?? [Re: Maverick]
    #16517067 - 07/11/12 10:09 PM (11 years, 7 months ago)

all that i have read suggests adding small amounts of peroxide after spore germination is beneficial in preventing contam.  many posts also suggest it for the same reason to be added just before storing established LC in the fridge.

are those sources missing something?


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OfflineMaverick
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Re: Is it TRUE?? Peroxide as replacement for filter disc in LC?? [Re: OysterFace]
    #16517272 - 07/11/12 10:46 PM (11 years, 7 months ago)

Considering you shouldn't have an issue with contamination provided your sterile technique is well practiced and you follow procedure, what's the point in adding something that oxidizes living tissue?  I haven't ever found it beneficial.  If you'd like to though, you should try, one with one without, there's nothing wrong with experimentation.


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OfflineRogerRabbitM
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Re: Is it TRUE?? Peroxide as replacement for filter disc in LC?? [Re: OysterFace]
    #16517388 - 07/11/12 11:07 PM (11 years, 7 months ago)

Quote:

OysterFace said:
sooo, can anyone actually answer the subject matter for which this post was written?




Peroxide is not a substitute for sterile procedure, nor is it a replacement for filters.
RR


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Offlinefatchillin
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Re: Is it TRUE?? Peroxide as replacement for filter disc in LC?? [Re: RogerRabbit]
    #16520483 - 07/12/12 02:44 PM (11 years, 7 months ago)

always listen to RR, hes the jedi master, agar > LC for eternity


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Invisible13shroomsM
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Re: Is it TRUE?? Peroxide as replacement for filter disc in LC?? [Re: fatchillin]
    #16520610 - 07/12/12 03:04 PM (11 years, 7 months ago)

:trololol:


peroxide = toxic to myc :themoreyouknow:



:smokeweedallday:


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Re: Is it TRUE?? Peroxide as replacement for filter disc in LC?? [Re: Maverick]
    #16520818 - 07/12/12 03:43 PM (11 years, 7 months ago)

Quote:

Maverick said: Really depends on how good you are with your sterile technique.




It's got nothing at all to do with sterile technique.
It's about working with the best choice or choosing the lesser option.

LC is the lesser option compared to agar. Or spores. Or any other method.


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OfflineMaverick
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Re: Is it TRUE?? Peroxide as replacement for filter disc in LC?? [Re: Doc_T]
    #16520906 - 07/12/12 04:00 PM (11 years, 7 months ago)

Quote:

Doc_T said:
Quote:

Maverick said: Really depends on how good you are with your sterile technique.




It's got nothing at all to do with sterile technique.
It's about working with the best choice or choosing the lesser option.

LC is the lesser option compared to agar. Or spores. Or any other method.




You're saying working with LCs and Agar don't have shit to do with sterile technique... :facepalm:

Is there a bigger facepalm feature?  Cuz it's needed for that statement.

First off, read my entire statement, second, stop saying dumb things.


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Invisible13shroomsM
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Re: Is it TRUE?? nope not at all. [Re: Maverick]
    #16522677 - 07/12/12 09:56 PM (11 years, 7 months ago)

:doublefacepalm:  :facepalm3:  :huxleyfacepalm:  :smbfacepalm:  :stanhopefacepalm:  :picard:





:greenthumb::vato:


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