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OfflineoO_wombat_Oo
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Something has gone wrong here... :( (updated)
    #1640451 - 06/17/03 09:10 PM (13 years, 9 months ago)

Edit:- Well, it's been a while since I posted this and still I have nothing. Not a spec of growth. I cracked one of the cakes open and it's certainly not contaminated. It smelled like bread dough, as you would expect. Should I chuck 'em all and start again?


Original post:

I'm not quite sure what the hell has happened here. I innoculated three jars a bit over a week ago, another three the next day and another two the day after that. Each day I used a slightly different substrate mix, and I used four different strains during the innoculation (so they're no all from the same syringe or even the same print).

It's been around five days since I innoculated the last jars and over a week since I did the first ones. Since then, not one jar has shown any sign of mycelial growth at all.

I'm hardly an expert cultivator, but I have done this before, and I'm baffled as to what it could be.

The jars have been kept in darkness at around 25-29 degrees C. The spore syringes where filled with normal tap water (sterilised, of course), and the spores where all allowed to re-hydrate for 24 hours prior to innoculation. The cakes are mainly made from organic flour and vermiculite, of couse I gave them 24 hours to cool after sterilisation.

There's one thing it could be, but I find this idea highly implausible. When I first put them into the incubation tub, I had cakes growing in there, so, to shield the jars from the light, I wrapped them in alfoil. Is it possible that, wrapped in alfoil, the jars got hot enough to kill the spores? I'm almost positive that is not it, but I'm so confused as to why it's not working...!


Edited by oO_wombat_Oo (06/19/03 11:38 PM)


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Invisibletak_old
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Re: Something has gone wrong here... :( [Re: oO_wombat_Oo]
    #1640456 - 06/17/03 09:12 PM (13 years, 9 months ago)

time will tell.


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Invisibledog
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Re: Something has gone wrong here... :( [Re: oO_wombat_Oo]
    #1640497 - 06/17/03 09:27 PM (13 years, 9 months ago)

Several possibilities come to mind.

If your spores were extremely old they might not be viable, but since you are using several different strains it seems very unlikey that they would all be too old.

If your tap water were extremely poor quality it might inhibit germination, but that also seems unlikely. My friend uses tap water with good results, and his is about as poor quality as it gets.

The only possibility that seems likely is contamination, probably bacterial. Crack the seal on one of your older jars and take a careful sniff. Does it smell like old beer or vinegar?

It's not the tin foil.


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OfflineoO_wombat_Oo
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Re: Something has gone wrong here... :( [Re: dog]
    #1640535 - 06/17/03 09:38 PM (13 years, 9 months ago)

Mkay then, I thought the tin foil was a silly idea anyway, but like I said, I'm pretty stumped on this one.

Thanks for your suggestions, but I think contamination is probably not it either. I find it unlikely that all the jars - despite having different substrates and spores, innoculated at different times - would be suffering from contamination, especially considering I've got a pretty good record avoiding contams.


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InvisibleBoppity604
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Re: Something has gone wrong here... :( [Re: oO_wombat_Oo]
    #1640637 - 06/17/03 10:13 PM (13 years, 9 months ago)

Patience. Give it at least 2 weeks before giving up on them.

Also...how did you prepare your syringes? If you used bleach or peroxide to clean out your syringes but didn't flush them out well enough with boiling water, you could have simply killed all your spores by leaving enough amounts of whatever you used to clean your syringes with (that's why I just used boiling water to clean/prep my syringes).

Best of luck...give it more time before throwing the jars out and starting over.

Love & Light,

Boppity


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OfflineoO_wombat_Oo
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Re: Something has gone wrong here... :( [Re: Boppity604]
    #1640702 - 06/17/03 10:47 PM (13 years, 9 months ago)

Thanks Boppity,

I just use boiling water to clean the syringes. And I'll take your advice and give it another four or five days. :wink:


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OfflineoO_wombat_Oo
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Re: Something has gone wrong here... :( [Re: oO_wombat_Oo]
    #1641407 - 06/18/03 04:19 AM (13 years, 9 months ago)

Hmmm, I just thought of something else. Probably not the problem, but maybe - what do you experts think?

I heat my incubation chamber with an aquarium heater, the jars just sit in about an inch or so of warm water. This water has a hefty helping of peroxide added to it, maybe this has somehow got into the jars (they're only drinking glasses with tin foil "lids") and killed the spores?


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OfflineoO_wombat_Oo
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Re: Something has gone wrong here... :( [Re: oO_wombat_Oo]
    #1646317 - 06/19/03 11:37 PM (13 years, 9 months ago)

bump.


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InvisibleLiL_KuSsH
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Deleted [Re: oO_wombat_Oo]
    #1646358 - 06/19/03 11:54 PM (13 years, 9 months ago)

Post deleted by administrator


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OfflineoO_wombat_Oo
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Re: Something has gone wrong here... :( [Re: LiL_KuSsH]
    #1646372 - 06/20/03 12:00 AM (13 years, 9 months ago)

yeah, I've heard of that before. Thing is, when birthing casings/cakes, I've got instant humidity this way. Don't have to fuck around with perlite or mistings or anything. Works a treat. That's why the peroxide is in there, to prevent cakes getting contamed. Anyway, maybe I should do it that way.


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