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Offlinedarkliight
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Registered: 04/29/12
Posts: 178
Last seen: 10 years, 6 months
Bulk Grow (H-Poo)
    #16367638 - 06/11/12 09:02 PM (11 years, 7 months ago)

Hello all,

I am finishing up the 3rd flush on the last batch of PF-style BRF cakes, and am looking to experiment with some bulk.

Here is what I have to work with. I am waiting on my spores to come in, I am expecting them around the end of the week.

Containers

- (2) 35 gallon Rubbermaid Roughneck (Latching lid/Stackable)
      = Dimensions:  81.3x50.8x49.8 cm

- (1) 31 gallon Rubbermaid Roughneck (Stackable)
      = Dimensions:  82.0x51.8x42.4 cm

- Polyfill

Spawn

- (12) 1 quart canning jars
- 10lbs Whole Rye Grain
- (2) Penis Envy Spore syringe
- Poly-Fill

Bulk Substrate

- (2) 5 gallon buckets of field-aged H-Poo
- 6 gallons Medium Grade Vermiculite
- 5lbs Garden Gypsum
- (5) 250g bricks of coco coir


I am using Prof_P's Bulk Poo Tek to create my bulk substrate, along with PSiLO187's Simple Bulk Spawn Production Tek for my spawn. I will keep posts in this thread, as well as more detailed posts in my journal, which I will have a link to in my signature.

Questions

So, I guess what I am looking to have answered is, do you guys think I am ready to go? (When my spores arrive, of course) I want to be sure I am not missing anything.

Also, Prof_P's Bulk Poo Tek uses 64 quart monotubs. My tubs are (1) 124qts and (2) 140qts. For the 124qt tub, I will just double the recipe (64x2=128). However, the 140qt is a little tricker, any advice on the ratio I should use?



I will be posting weekly updates with pictures on this grow for a more detailed grow report please see my journal (linked in sig).


Thanks for the help!


--------------------
"Excellence in any department can be attained only by the labor of a lifetime; it is not to be purchased at a lesser price."
- Samuel Johnson

New growers: Feel free to PM/e-mail with questions!
Email: darkliight@shroomery.org

First Grow - BRF Golden Teachers
Second Grow - Prof_P Tek PE

Everything I say on this site is a lie.
Let the paradox begin.


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Offlinereinfect
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Registered: 05/14/12
Posts: 207
Last seen: 6 years, 17 days
Re: Bulk Grow (H-Poo) [Re: darkliight]
    #16368159 - 06/11/12 10:55 PM (11 years, 7 months ago)

How are you going to prepare your lids?
Also, I'd say go ahead and grab another 12-pack of quart jars, G2G is rad.


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InvisibleFrankHorrigan
The Inquisition
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Registered: 01/04/11
Posts: 10,573
Trusted Cultivator
Re: Bulk Grow (H-Poo) [Re: reinfect]
    #16368194 - 06/11/12 11:01 PM (11 years, 7 months ago)

Pasteurize your substrate in jars via RR's method.  That way, you can know your exact ratio of spawn:sub.  1:1 is fastest and will give you hefty flushes, but 1:2 or 1:3 or even 1:4 is fine.
I usually go 1:2.


--------------------

Yes, you can bump my old threads with a question.
Here is how I get things done.
You should take a look. :hehehe:


Frank's tips and tricks. Updated on 3/21/14
AMU- Get an answer here -AMU


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Offlinedarkliight
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Re: Bulk Grow (H-Poo) [Re: FrankHorrigan]
    #16370509 - 06/12/12 12:00 PM (11 years, 7 months ago)

Quote:

reinfect said:
How are you going to prepare your lids?
Also, I'd say go ahead and grab another 12-pack of quart jars, G2G is rad.




I am going to use RR's method for jar preparation. One larger hole in each lid to hold the polyfill, I will just inoculate around the polyfill.

You know you are right, I found some fairly cheap recently. Only $10 bucks for a 12-pack of 1qt. jars.

Quote:

FrankHorrigan said:
Pasteurize your substrate in jars via RR's method.  That way, you can know your exact ratio of spawn:sub.  1:1 is fastest and will give you hefty flushes, but 1:2 or 1:3 or even 1:4 is fine.
I usually go 1:2.




Would you happen to know of a link detailing RR's pasteurization process? I know the basics (PC @ 180F for 120 minutes), but was wanting to find a good detailed post covering the topic.

As far as my spawn:substrate ratio, I would like to use a 1:1 ratio for the hearty first flushes and quicker colonization of the bulk substrate. Is there anyway to figure out how much bulk substrate will be used per tub, before preparing it? Using the given dimensions?


--------------------
"Excellence in any department can be attained only by the labor of a lifetime; it is not to be purchased at a lesser price."
- Samuel Johnson

New growers: Feel free to PM/e-mail with questions!
Email: darkliight@shroomery.org

First Grow - BRF Golden Teachers
Second Grow - Prof_P Tek PE

Everything I say on this site is a lie.
Let the paradox begin.


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InvisibleFrankHorrigan
The Inquisition
Male


Registered: 01/04/11
Posts: 10,573
Trusted Cultivator
Re: Bulk Grow (H-Poo) [Re: darkliight]
    #16370715 - 06/12/12 12:40 PM (11 years, 7 months ago)

Quote:

darkliight said:
Quote:

FrankHorrigan said:
Pasteurize your substrate in jars via RR's method.  That way, you can know your exact ratio of spawn:sub.  1:1 is fastest and will give you hefty flushes, but 1:2 or 1:3 or even 1:4 is fine.
I usually go 1:2.




Would you happen to know of a link detailing RR's pasteurization process? I know the basics (PC @ 180F for 120 minutes), but was wanting to find a good detailed post covering the topic.

As far as my spawn:substrate ratio, I would like to use a 1:1 ratio for the hearty first flushes and quicker colonization of the bulk substrate. Is there anyway to figure out how much bulk substrate will be used per tub, before preparing it? Using the given dimensions?




mushroomvideos.com
Watch the h-poo videos.
You'll need to get some extra quart jars. It's safe to go bigger than necessary for the first time so you'll have enough substrate. A good starting point would be 15-18 quarts of (hydrated) substrate and 8-12 quarts of spawn, tho if you're looking at one-to-one ration you'll want more spawn.  A well made 35 gallon tub should give you at least 6-8oz dry PE, so if you're not wanting to spend a lot more money you can always make up one tub and then buy more supplies after if it goes to your liking.
In case you make up too much spawn, just toss it into your compost pile or yard. 
Pasteurizing in jars is simple, you load your quart jars with substrate to the top, put foil over it, no lid.  Make sure you have a meat thermometer handy to insert as close to the center of the jar as possible, this measures your sub's internal temp.  Fill a stockpot with your substrate jars, and fill with water 2/3-3/4 of the way up the jars. 
I deviate a bit from RR's method here: I turn my stove on medium and wait for the temp probe to hit 120, then I turn off the heat and let the pot sit.  When the internal temp hits 140, start a 60 minute timer.  When the timer goes off, make sure you're still above 140 and remove the jars from the water to cool overnight. I put them in a tub but any old shelf will do.
I do this because if I let the water boil as RR says to, my temps get into the 190 range and this defeats the whole point of pasteurizing.  If your temp gets about 170, throw the substrate away and try again.  Partial sterilization will occur and you open yourself up to contams.
I hope this helps!  Good luck!


--------------------

Yes, you can bump my old threads with a question.
Here is how I get things done.
You should take a look. :hehehe:


Frank's tips and tricks. Updated on 3/21/14
AMU- Get an answer here -AMU


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OfflinePinheadX
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Registered: 04/25/07
Posts: 1,414
Loc: TX Gulf Coast
Last seen: 6 years, 1 month
Re: Bulk Grow (H-Poo) [Re: FrankHorrigan]
    #16371026 - 06/12/12 01:46 PM (11 years, 7 months ago)

I'm just wondering...

if you're growing cubes now, why did you order spores?

Why not make a sporeprint and use the old syringes to make up new ones?

Recycle, man!!!

I totally understand if you're looking to grow a specific strain (you said PE, so I get that...), but if you're doing multispore inoculations anyway, why not use the spores from the mushrooms you are already growing? Just seems like a no brainer to me.


--------------------
If you want to find psilocybin in species that are not yet known to be psychoactive, you should do chemical tests. That way you won't get sick and die all the time.
- Alan Rockefeller

Reality is that which, when you stop believing in it, doesn't go away."
- Philip K. Dick


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Offlinedarkliight
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Registered: 04/29/12
Posts: 178
Last seen: 10 years, 6 months
Re: Bulk Grow (H-Poo) [Re: PinheadX]
    #16372288 - 06/12/12 06:02 PM (11 years, 7 months ago)

Quote:

FrankHorrigan said:
mushroomvideos.com
Watch the h-poo videos.
You'll need to get some extra quart jars. It's safe to go bigger than necessary for the first time so you'll have enough substrate. A good starting point would be 15-18 quarts of (hydrated) substrate and 8-12 quarts of spawn, tho if you're looking at one-to-one ration you'll want more spawn.  A well made 35 gallon tub should give you at least 6-8oz dry PE, so if you're not wanting to spend a lot more money you can always make up one tub and then buy more supplies after if it goes to your liking.
In case you make up too much spawn, just toss it into your compost pile or yard. 
Pasteurizing in jars is simple, you load your quart jars with substrate to the top, put foil over it, no lid.  Make sure you have a meat thermometer handy to insert as close to the center of the jar as possible, this measures your sub's internal temp.  Fill a stockpot with your substrate jars, and fill with water 2/3-3/4 of the way up the jars. 
I deviate a bit from RR's method here: I turn my stove on medium and wait for the temp probe to hit 120, then I turn off the heat and let the pot sit.  When the internal temp hits 140, start a 60 minute timer.  When the timer goes off, make sure you're still above 140 and remove the jars from the water to cool overnight. I put them in a tub but any old shelf will do.
I do this because if I let the water boil as RR says to, my temps get into the 190 range and this defeats the whole point of pasteurizing.  If your temp gets about 170, throw the substrate away and try again.  Partial sterilization will occur and you open yourself up to contams.
I hope this helps!  Good luck!




Thanks for the info and the link, Frank! It has been raining here recently so my h-poo is damp. It has been aging for a couple months now, so I think its ready. We have nice hot, sunny weather ahead of us, so it'll dry up in no time. Now, when I get ready to start pasteurizing I'll feel a little more comfortable now, after reading your post.

Quote:

PinheadX said:
I'm just wondering...

if you're growing cubes now, why did you order spores?

Why not make a sporeprint and use the old syringes to make up new ones?

Recycle, man!!!

I totally understand if you're looking to grow a specific strain (you said PE, so I get that...), but if you're doing multispore inoculations anyway, why not use the spores from the mushrooms you are already growing? Just seems like a no brainer to me.




I am wanting to try out a new strain. My last batch, were Golden Teachers. Besides, I heard a cube is a cube, unless it's PE, so hence me buying a new syringe.

I've already made several spore prints and added them to my collection. I also took some prints of Amanita muscaria var. formosa and Amanita bisporigera the same day I made my GT prints.

You are right, though, I will be recycling these GTs soon perhaps for another batch of PF-cakes. I know they are for beginners, but I love the simplicity, and I get a decent batch while also spending time with my shroomies :pipesmoke:


--------------------
"Excellence in any department can be attained only by the labor of a lifetime; it is not to be purchased at a lesser price."
- Samuel Johnson

New growers: Feel free to PM/e-mail with questions!
Email: darkliight@shroomery.org

First Grow - BRF Golden Teachers
Second Grow - Prof_P Tek PE

Everything I say on this site is a lie.
Let the paradox begin.


Edited by darkliight (06/12/12 10:55 PM)


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InvisibleFrankHorrigan
The Inquisition
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Registered: 01/04/11
Posts: 10,573
Trusted Cultivator
Re: Bulk Grow (H-Poo) [Re: darkliight]
    #16372386 - 06/12/12 06:21 PM (11 years, 7 months ago)

No problem!
I did have a typo tho:
I said if you make up too much spawn, throw it to compost, but I meant to say if you make up too much SUBSTRATE, throw it to compost.  If you have too much spawn, just throw it in the tub!
:thumbup:


--------------------

Yes, you can bump my old threads with a question.
Here is how I get things done.
You should take a look. :hehehe:


Frank's tips and tricks. Updated on 3/21/14
AMU- Get an answer here -AMU


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Offlinedarkliight
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Registered: 04/29/12
Posts: 178
Last seen: 10 years, 6 months
Re: Bulk Grow (H-Poo) [Re: darkliight]
    #16401194 - 06/18/12 10:49 PM (11 years, 7 months ago)

June 19th Update

So, I prepared my jars and grain a couple of days ago. I let my rye grain soak for around 14 hours, loaded my jars and PC'd them for 70 minutes each, and allowed the PC to depressurize itself.

On June 15th, I inoculated 3 jars with 1cc of MS solution. On June 18th (last night) I prepared 15 more spawn jars under the exact same conditions as the first 3. I plan to inoculate tomorrow after work. I will be making more jars but not until some of the jars I have start colonizing.

Will update as I continue with my grow.


--------------------
"Excellence in any department can be attained only by the labor of a lifetime; it is not to be purchased at a lesser price."
- Samuel Johnson

New growers: Feel free to PM/e-mail with questions!
Email: darkliight@shroomery.org

First Grow - BRF Golden Teachers
Second Grow - Prof_P Tek PE

Everything I say on this site is a lie.
Let the paradox begin.


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Offlinedarkliight
Aborted
Male


Registered: 04/29/12
Posts: 178
Last seen: 10 years, 6 months
Re: Bulk Grow (H-Poo) [Re: darkliight]
    #16443210 - 06/26/12 10:30 PM (11 years, 7 months ago)

June 26th Update

Alright, so today was quite productive! I inoculated 7 more jars,using my new glovebox that I made (pics below). I feel very confident and much more at ease using the glove box to ensure that optimal sterility is met.

However, while I got a lot done, there was also work that was undone. One of the quart jars that I inoculated with my MS syringe was contaminated with Trichoderma. Upon discovery, I quickly disposed of the rye and the put the jar in the pressure cooker for a quick sterilization. The first three jars were inoculated on June 17th and are about 10-15% colonized, based on an examination of the contaminated jars. The internal rye was much more colonized than was the outer, visible layer of rye. These three jars were done open air, as I had not made my glovebox yet.

Pictures



Ready for inoculations and grain to grain transfers now :laugh:

I know, it's no laminar flow hood, but it is a lot better than open air work.



I added flaps to help ensure that no contams float in while I am flame sterilizing my needle.



Just so no one is confused. The jars with tape over the holes have not been inoculated yet. I am using polyfill as my filter.


--------------------
"Excellence in any department can be attained only by the labor of a lifetime; it is not to be purchased at a lesser price."
- Samuel Johnson

New growers: Feel free to PM/e-mail with questions!
Email: darkliight@shroomery.org

First Grow - BRF Golden Teachers
Second Grow - Prof_P Tek PE

Everything I say on this site is a lie.
Let the paradox begin.


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Offlinesgfcchamber
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Registered: 10/15/11
Posts: 554
Last seen: 7 years, 9 months
Re: Bulk Grow (H-Poo) [Re: darkliight]
    #16443276 - 06/26/12 10:48 PM (11 years, 7 months ago)

Quote:

darkliight said:
Would you happen to know of a link detailing RR's pasteurization process? I know the basics (PC @ 180F for 120 minutes), but was wanting to find a good detailed post covering the topic.




http://www.shroomery.org/forums/showflat.php/Number/11855255
That's a link to an illustrated pasteurization tek that is basically 100% the same as RR's method (I've got the DVD's, and comparing what RR does on the DVD's to that thread, there are basically no differences).

Quote:

darkliight said:
As far as my spawn:substrate ratio, I would like to use a 1:1 ratio for the hearty first flushes and quicker colonization of the bulk substrate. Is there anyway to figure out how much bulk substrate will be used per tub, before preparing it? Using the given dimensions?




Check out this link:

http://SubstrateCalculator.info

You can find a link to a thread here on Shroomery about that site in my sig.

Hope those links help, and good luck on your grow!

Looks like you're well on your way to success :laugh:


--------------------

========================================================================================
Mushroom / Bulk Substrate Calculator <- Tells you exactly how much of what ingredients you need for any substrate for any container!
Here is the corresponding shroomery thread: http://www.shroomery.org/forums/showflat.php/Number/15656577


Easy Bulk Substrate Pasteurizer - Hands Free Operation
========================================================================================


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Offlinedarkliight
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Registered: 04/29/12
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Re: Bulk Grow (H-Poo) [Re: sgfcchamber]
    #16443346 - 06/26/12 11:08 PM (11 years, 7 months ago)

Quote:

sgfcchamber said:
http://www.shroomery.org/forums/showflat.php/Number/11855255
That's a link to an illustrated pasteurization tek that is basically 100% the same as RR's method (I've got the DVD's, and comparing what RR does on the DVD's to that thread, there are basically no differences).




I actually went ahead and purchased RR's videos. What a great investment! You are right there is practically no difference in this part of the set.


Quote:

sgfcchamber said:
Check out this link:

http://SubstrateCalculator.info

You can find a link to a thread here on Shroomery about that site in my sig.

Hope those links help, and good luck on your grow!

Looks like you're well on your way to success :laugh:




Thanks so much! I broke open my old college algebra book and did my volume calculations. I still haven't decided what my ratio will be this is what I got so far:

(2) - 35 gal. (205.675L) tubs
32L x 20W x 49.8H in.

Substrate depth: 4 in.
Substrate volume: 41.92L (Right?)



...and the 3rd tub (which at first I thought was smaller, but actually has more surface area for substrate, it is just shorter)

(1) - 31 gal. (201.92L) tub
32.3L x 20.4W x 18.7H

Substrate depth: 4 in.
Substrate volume: 43.19L


So that would put my ratios for the first two (35 gal) tubs at:

1:1 Ratio - 42 qts of spawn
1:2 Ratio - 21 qts of spawn
1:3 Ratio - 14 qts of spawn
1:4 Ratio - 10.5 qts of spawn

...and the third at:

1:1 Ratio - 43 qts of spawn
1:2 Ratio - 21.5 qts of spawn
1:3 Ratio - 14 qts of spawn
1:4 Ratio - 10.75 qts of spawn


Right? Lol


--------------------
"Excellence in any department can be attained only by the labor of a lifetime; it is not to be purchased at a lesser price."
- Samuel Johnson

New growers: Feel free to PM/e-mail with questions!
Email: darkliight@shroomery.org

First Grow - BRF Golden Teachers
Second Grow - Prof_P Tek PE

Everything I say on this site is a lie.
Let the paradox begin.


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