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OfflineDionysus
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Registered: 06/11/03
Posts: 117
Loc: Canada
Last seen: 12 years, 7 months
Substituting spore syringes with sporeprints
    #1635459 - 06/15/03 06:25 PM (13 years, 7 months ago)

Is there a way to use sporeprints instead of spore syringes?
Could the Tek method be modified for sporeprints?  :confused:
I have a problem, I am leaving twon in early August. I want to grow some shrooms beforehand (and hopefully have enough time to use them). It will take me at least a couple weeks to get a spore syringe. I have right now a sporeprint. Can I use this in someway so I can get a headstart on my cultivation?
I need any advice or help whatsoever!
Thank you.  :laugh: 


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InvisibleSixCee
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Registered: 06/12/02
Posts: 3,720
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Re: Substituting spore syringes with sporeprints [Re: Dionysus]
    #1635462 - 06/15/03 06:27 PM (13 years, 7 months ago)

If you have a glove box or flow hood you can scrape some spores off of the print into the jar. Doing this without the mentioned equipment will usually result in failure.


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OfflineDionysus
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Registered: 06/11/03
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Loc: Canada
Last seen: 12 years, 7 months
Re: Substituting spore syringes with sporeprints [Re: SixCee]
    #1635467 - 06/15/03 06:28 PM (13 years, 7 months ago)

Just scrap it on top of the vermaculite then mix it around, or what?


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"To arrive at the edge of the world's knowledge, seek out the most complex and sophisticated minds, put them in a room together, and have them ask each other the questions they are asking themselves."


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OfflineFallenShroom
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Registered: 05/01/03
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Re: Substituting spore syringes with sporeprints [Re: Dionysus]
    #1635474 - 06/15/03 06:29 PM (13 years, 7 months ago)

Sure try http://www.shroomery.org/index/par/22982 popcorn tekand scrape spores into this use a glove box pf tek method you will have a problem


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InvisibleSixCee
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Re: Substituting spore syringes with sporeprints [Re: FallenShroom]
    #1635487 - 06/15/03 06:34 PM (13 years, 7 months ago)

The spores must come in contact with substarte.


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->Quote of the Moment :
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OfflineDionysus
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Registered: 06/11/03
Posts: 117
Loc: Canada
Last seen: 12 years, 7 months
Re: Substituting spore syringes with sporeprints [Re: SixCee]
    #1635498 - 06/15/03 06:39 PM (13 years, 7 months ago)

So, after the popcorn grain has been sterilized in pressure cooker, just scrap the spores directly into the jar and shake it up? Is this correct?


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"To arrive at the edge of the world's knowledge, seek out the most complex and sophisticated minds, put them in a room together, and have them ask each other the questions they are asking themselves."


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OfflineDionysus
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Registered: 06/11/03
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Last seen: 12 years, 7 months
Re: Substituting spore syringes with sporeprints [Re: Dionysus]
    #1635640 - 06/15/03 07:38 PM (13 years, 7 months ago)

Has anyone used a sporeprint instead of a spore syringe?


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"To arrive at the edge of the world's knowledge, seek out the most complex and sophisticated minds, put them in a room together, and have them ask each other the questions they are asking themselves."


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OfflineFallenShroom
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Re: Substituting spore syringes with sporeprints [Re: Dionysus]
    #1635675 - 06/15/03 07:55 PM (13 years, 7 months ago)

yes i have tested this took longer to see results compared to my usual corn syrup tek


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Invisiblekoraks
Registered: 06/02/03
Posts: 25,087
Re: Substituting spore syringes with sporeprints [Re: FallenShroom]
    #1636966 - 06/16/03 10:18 AM (13 years, 7 months ago)

I think it's best to make a spore syringe from your spore print. This is relatively easy and safe, and it enables you to follow the tek.
Here's how I do it:
1. Sterilize a syringe and a needle.
2. Sterilize a glass (wine glass, shot glass, whatever).
3. Cook some water, and suck it into the syringe. This way you know you have enough water for one syringe. You could make several syringes from a spore print, in that case, repeat steps 3 and 4 for the number of syringes you want to make.
4. Let the water flow into the glass.
5. Cover the glass with some tinfoil.
6. Let the water cool.
7. Take the sporeprint and scrape the spores into the glass.
8. Suck the water with the spores into the syringe(s). You can suck some water up the syringe and squirt it back in order to stir the suspension.

This is a very simple tek for making spore syringes, but the contamination risk is higher than with many other teks. To reduce contam risks, work in a clean environment (clean with alcohol/bleach/lysol), or use a glovebox.


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OfflineDionysus
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Registered: 06/11/03
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Loc: Canada
Last seen: 12 years, 7 months
Re: Substituting spore syringes with sporeprints [Re: koraks]
    #1637280 - 06/16/03 01:56 PM (13 years, 7 months ago)

Thank you for the advice.
However, I don't have a syringe at all. That is why I asked about the sporeprint itself. I've heard of people scrapping spores from the sporeprint right into the jar. Can I do this? Will it work?


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"To arrive at the edge of the world's knowledge, seek out the most complex and sophisticated minds, put them in a room together, and have them ask each other the questions they are asking themselves."


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OfflineSev
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Registered: 06/06/03
Posts: 1,426
Loc: NY
Last seen: 2 years, 3 months
Re: Substituting spore syringes with sporeprints [Re: Dionysus]
    #1637297 - 06/16/03 02:03 PM (13 years, 7 months ago)

Yes, it'll work, if you scrape the spores directly onto the substrate.  It would be better, (read, faster to colonize and less likely to contaminate,) however, if you made a syringe -- all you need, after all, is a syringe.  :wink: 


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"Do we want the stars? We can have them. Can we borrow cups of fire from the sun? We can and must and light the world." --"On the Shoulders of Giants", Ray Bradbury

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OfflineDionysus
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Registered: 06/11/03
Posts: 117
Loc: Canada
Last seen: 12 years, 7 months
Re: Substituting spore syringes with sporeprints [Re: Sev]
    #1639976 - 06/17/03 05:15 PM (13 years, 7 months ago)

I'll try scraping directly onto prepared corn seeds. This should work.


--------------------
"To arrive at the edge of the world's knowledge, seek out the most complex and sophisticated minds, put them in a room together, and have them ask each other the questions they are asking themselves."


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Offlinedizzim
Born Again Head
Registered: 04/30/03
Posts: 238
Last seen: 2 years, 1 month
Re: Substituting spore syringes with sporeprints [Re: Dionysus]
    #1640188 - 06/17/03 06:38 PM (13 years, 7 months ago)

It should be fine.  My friend has never used syringes and has had success with scraping prints and then shaking the jars.  This method is more open to contams but works.  My friend has some batches where he loses 2 or 3 out of 5, and others where all are successfull.  Only open the jar you're about to scrape spores into and sterilize your scraping instrument after each jar - lately my friend hasnt lost any :smile:

dizz


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