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Offlinejonniex
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Registered: 11/16/02
Posts: 30
Last seen: 14 years, 10 months
Creating a small enclosed space to prevent contamination
    #1635261 - 06/15/03 04:50 PM (15 years, 6 days ago)

Hello I recently had this great idea....

Basically my last batch of mushrooms was ruined due to mold so in addition to buying filters, etc I came up with the idea that during the critical phase where I cut up the cakes and sandwich them between a thin layer of vermiculite I perform this in a small sealed temporary structure.

I was thinking of buying a tent 5 1/2 feet squared and sealed off the vents.
Before doing anything I'd spray the whole thing down with Lysol and put a HEPA filter inside. Then I'd go to work in a sterile environment.

The problem :confused: I can't seem to find a structure suitable for this.
It seems most tents would just collapse on themselves.

Does anyone have any ideas of what else I could purchase (maybe on ebay) for a fair price that would do the trick. I don't have the patience to construct anything.

Thanks and feel free to use this idea even though its mere commonsense.

Jonathan
 


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OfflinefIsh in my head
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Registered: 01/25/03
Posts: 1,150
Loc: 4500 ASL
Last seen: 4 months, 26 days
Re: Creating a small enclosed space to prevent contamination [Re: jonniex]
    #1635289 - 06/15/03 04:58 PM (15 years, 6 days ago)


Well, the glove box would have been a great idea but since you don't have patience for anything, i think there's not a lot of options available.

Just work in your bathroom then with all your stuff prepared so you don't have to leave the room. Close the door and spray everywhere like a crazy hypocondriac and start your work.

I don't feel the need to build a glove box me neither and so far, I have not a lot contamination problems. I recently made a syringe and didn't test it yet. I don't know if it's sterile but I think if you're careful enough and use some lysol, alcohol and gloves you can be sterile enough.


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Offlinejonniex
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Registered: 11/16/02
Posts: 30
Last seen: 14 years, 10 months
Re: Creating a small enclosed space to prevent contamination [Re: fIsh in my head]
    #1635292 - 06/15/03 04:59 PM (15 years, 6 days ago)

MY BATHROOM, haha. Oh lord I'd end up growing martians


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OfflinefIsh in my head
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Registered: 01/25/03
Posts: 1,150
Loc: 4500 ASL
Last seen: 4 months, 26 days
Re: Creating a small enclosed space to prevent contamination [Re: jonniex]
    #1635298 - 06/15/03 05:01 PM (15 years, 6 days ago)


Eh eh eh...

just buy some patience on eBay and either build glovebox or clean your martian spores !


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Offlinejonniex
Male
Registered: 11/16/02
Posts: 30
Last seen: 14 years, 10 months
Re: Creating a small enclosed space to prevent contamination [Re: fIsh in my head]
    #1635333 - 06/15/03 05:11 PM (15 years, 6 days ago)

a glove box seems like a good idea but in a sense not the best.

It seems like doing all this in a little box would be difficult.

Especially because my technique which I have had success with is cutting the cakes on a plate and sticking them on the little layer of vermiculate.

The dumb this is I did this all out in the open with just a spritz of lysol now and again, not exactly perfect.

So you can see why I think this is such a good idea.

I need space to move around while i do this, jump, dance, etc HAHA


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OfflineDemiurge
creator ofworlds

Registered: 04/11/03
Posts: 693
Loc: Florida
Last seen: 7 years, 2 months
Re: Creating a small enclosed space to prevent contamination [Re: jonniex]
    #1886473 - 09/05/03 09:09 PM (14 years, 9 months ago)

If you have even a small fan with a hepa filter on it blowing into a big clear garbage bag it won't colapse. Just make sure it's taped around your hands with only a little place for air to escape. This creates a simple positve pressure sterile environment. You don't even have to lysol the bag, cause they're sterile right out of the box.


--------------------
Just another animal embeded in a technological coral reef extruded psychic objects...


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