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jonniex
Male
Registered: 11/16/02
Posts: 30
Last seen: 20 years, 6 months
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Creating a small enclosed space to prevent contamination
#1635261 - 06/15/03 02:50 PM (20 years, 7 months ago) |
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Hello I recently had this great idea....
Basically my last batch of mushrooms was ruined due to mold so in addition to buying filters, etc I came up with the idea that during the critical phase where I cut up the cakes and sandwich them between a thin layer of vermiculite I perform this in a small sealed temporary structure.
I was thinking of buying a tent 5 1/2 feet squared and sealed off the vents. Before doing anything I'd spray the whole thing down with Lysol and put a HEPA filter inside. Then I'd go to work in a sterile environment.
The problem I can't seem to find a structure suitable for this. It seems most tents would just collapse on themselves.
Does anyone have any ideas of what else I could purchase (maybe on ebay) for a fair price that would do the trick. I don't have the patience to construct anything.
Thanks and feel free to use this idea even though its mere commonsense.
Jonathan
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fIsh in my head
fadedstar

Registered: 01/25/03
Posts: 1,150
Loc: 4500 ASL
Last seen: 6 years, 11 days
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Re: Creating a small enclosed space to prevent contamination [Re: jonniex]
#1635289 - 06/15/03 02:58 PM (20 years, 7 months ago) |
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Well, the glove box would have been a great idea but since you don't have patience for anything, i think there's not a lot of options available.
Just work in your bathroom then with all your stuff prepared so you don't have to leave the room. Close the door and spray everywhere like a crazy hypocondriac and start your work.
I don't feel the need to build a glove box me neither and so far, I have not a lot contamination problems. I recently made a syringe and didn't test it yet. I don't know if it's sterile but I think if you're careful enough and use some lysol, alcohol and gloves you can be sterile enough.
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jonniex
Male
Registered: 11/16/02
Posts: 30
Last seen: 20 years, 6 months
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Re: Creating a small enclosed space to prevent contamination [Re: fIsh in my head]
#1635292 - 06/15/03 02:59 PM (20 years, 7 months ago) |
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MY BATHROOM, haha. Oh lord I'd end up growing martians
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fIsh in my head
fadedstar

Registered: 01/25/03
Posts: 1,150
Loc: 4500 ASL
Last seen: 6 years, 11 days
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Re: Creating a small enclosed space to prevent contamination [Re: jonniex]
#1635298 - 06/15/03 03:01 PM (20 years, 7 months ago) |
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Eh eh eh... just buy some patience on eBay and either build glovebox or clean your martian spores !
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jonniex
Male
Registered: 11/16/02
Posts: 30
Last seen: 20 years, 6 months
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Re: Creating a small enclosed space to prevent contamination [Re: fIsh in my head]
#1635333 - 06/15/03 03:11 PM (20 years, 7 months ago) |
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a glove box seems like a good idea but in a sense not the best.
It seems like doing all this in a little box would be difficult.
Especially because my technique which I have had success with is cutting the cakes on a plate and sticking them on the little layer of vermiculate.
The dumb this is I did this all out in the open with just a spritz of lysol now and again, not exactly perfect.
So you can see why I think this is such a good idea.
I need space to move around while i do this, jump, dance, etc HAHA
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Demiurge
creator ofworlds

Registered: 04/11/03
Posts: 693
Loc: Florida
Last seen: 12 years, 10 months
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Re: Creating a small enclosed space to prevent contamination [Re: jonniex]
#1886473 - 09/05/03 07:09 PM (20 years, 4 months ago) |
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If you have even a small fan with a hepa filter on it blowing into a big clear garbage bag it won't colapse. Just make sure it's taped around your hands with only a little place for air to escape. This creates a simple positve pressure sterile environment. You don't even have to lysol the bag, cause they're sterile right out of the box.
-------------------- Just another animal embeded in a technological coral reef extruded psychic objects...
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