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Amazon Shop for: ½ Pint Jars, Brown Rice Flour, Vermiculite, Wild Bird Seed

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Offlinecyndyl
mycologist wannabe
Female

Registered: 05/26/03
Posts: 193
Loc: far away from home
Last seen: 5 years, 10 months
assorted newbee questions
    #1634683 - 06/15/03 09:35 AM (13 years, 5 months ago)

I've just started my second try at this. This time trying the PF method...
I sterilized my jars with industral strength tape over the holes. After innoculating should I remove the tape or is the holes made by the syringe enough air? don't want to let the contams in.

Secondly, what's the biz with shaking? Should I shake these jars at some point? I know they're not to be shaken after they're well under way.

One third question is about sterilizing the work area... I've got bleach, 91% alcohol, hydrogen peroxide and lysol. I've been washing out containers with bleach/water, spraying work areas with alcohol and lysol, & using alcohol to wipe down the tops of jars before innoculation and the syringe. Should I be spraying both lysol and alcohol or just stick to one?


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InvisibleOJK
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Registered: 06/08/03
Posts: 10,629
Re: assorted newbee questions [Re: cyndyl]
    #1634689 - 06/15/03 09:44 AM (13 years, 5 months ago)

Lysol and alchol fufill the same role, using one should be fine. The tape / holes question has no definitive answer... personally, I leave the holes in my jar unblocked, but some people leave tape on until the mycelium starts to grow. If your worried about contams, you can stick filter material over the holes. If your using the pf tek, you don't need to shake the jars (assuming your using a brown rice flour / vermiculite substrate), you only shake the jars if your using a grain substrate (e.g wild bird seed)


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Offlinecyndyl
mycologist wannabe
Female

Registered: 05/26/03
Posts: 193
Loc: far away from home
Last seen: 5 years, 10 months
Re: assorted newbee questions [Re: OJK]
    #1634801 - 06/15/03 11:48 AM (13 years, 5 months ago)

One more question...
The spores are Golden Teacher and one cannot see any dark stuff in the syringe. Are Golden Teacher's particularly small?
I've used Pink Buffalo and Philospher Stones before. Both were very cloudy and required a good deal of shaking.
I'm not going to worry until I don't see any activity for a week. The provider of the spores is the same that has provided me with another project, which is going along nicely. One of the shroomry sponsors.


--------------------


There's no such thing as a stupid question,
but they're the easiest to answer!


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Offlinecyndyl
mycologist wannabe
Female

Registered: 05/26/03
Posts: 193
Loc: far away from home
Last seen: 5 years, 10 months
Re: assorted newbee questions [Re: OJK]
    #1634805 - 06/15/03 11:50 AM (13 years, 5 months ago)

Thank you Odiumjunkie! I'm worring over this batch like a mother hen.


--------------------


There's no such thing as a stupid question,
but they're the easiest to answer!


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Invisiblekoraks
Registered: 06/02/03
Posts: 25,083
Re: assorted newbee questions [Re: cyndyl]
    #1635172 - 06/15/03 03:56 PM (13 years, 5 months ago)

Don't worry if you see nothing but clear water in a sytinge. Spores are a couple of microns large, and there can be millions of them in your syringe without you even seeing them. Just go along as planned, and only start worrying when nothing happens after about ten days.


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Amazon Shop for: ½ Pint Jars, Brown Rice Flour, Vermiculite, Wild Bird Seed

Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation

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