Home | Community | Message Board

Sporeworks
This site includes paid links. Please support our sponsors.


Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: OlympusMyco.com No Unicorns Here—Just Quality Bags That Work   North Spore Injection Grain Bag   Left Coast Kratom Buy Kratom Extract   MagicBag.co All-In-One Bags That Don't Suck   Original Sensible Seeds Bulk Cannabis Seeds   PhytoExtractum Maeng Da Thai Kratom Leaf Powder

Jump to first unread post Pages: 1
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
Offlinemadis503
psühhonaut


Registered: 07/29/09
Posts: 466
Loc: Flag
Last seen: 2 years, 30 days
MEYA problem
    #16339789 - 06/06/12 05:52 AM (12 years, 7 months ago)

Hi all!
i tried cloning with cardboard and it didnt work out, so now i did it with agar and it seems that i have failed again. the mushroom tissue has not grown. on some plates i see bacteria growing but no mycelium on any of the dishes.
the recipe was 200ml water, 4 g malt, 3,5g agar agar, 1g yeast.
i suspect that i bought something malt-like? Does it has to be powder too?


yeast and agar:


i dont have parafilm yet but i ordered a roll and a box of 3 sector petri dishes (480) i just dont want to fail anymore. those dishes have been sitting for 4 days now :frown:
these are sealed with micropore tape.




--------------------

Psy&Goa

Extras: Filter Print Post Top
InvisibleDoc_T
Random Dude
Male User Gallery


Folding@home Statistics
Registered: 03/06/09
Posts: 42,395
Loc: Colorado Flag
Re: MEYA problem [Re: madis503]
    #16339821 - 06/06/12 06:22 AM (12 years, 7 months ago)

You are successfully growing bacteria, that's a good first step.
What kind of tissue cloning are you doing? A wild specimen?


--------------------
You make it all possible. Doesn't it feel good?

Extras: Filter Print Post Top
Offlinemadis503
psühhonaut


Registered: 07/29/09
Posts: 466
Loc: Flag
Last seen: 2 years, 30 days
Re: MEYA problem [Re: Doc_T]
    #16339982 - 06/06/12 07:32 AM (12 years, 7 months ago)

no no, just picked 2 of the ones i liked the most and cloned them, but they were in the refridgerator for 4 days. but do i have the right malt? :laugh:
and if theres bacteria growing then myc wont grow at all?
and can i re melt the agar what was left over?


--------------------

Psy&Goa

Extras: Filter Print Post Top
InvisibleDoc_T
Random Dude
Male User Gallery


Folding@home Statistics
Registered: 03/06/09
Posts: 42,395
Loc: Colorado Flag
Re: MEYA problem [Re: madis503]
    #16339998 - 06/06/12 07:38 AM (12 years, 7 months ago)

Quote:

madis503 said:
but do i have the right malt? :laugh:




You have maltose, refined malt sugar. It will work ok, especially with the added yeast.

Quote:

madis503 said:
and if theres bacteria growing then myc wont grow at all?




Maybe myc will grow and if so you can transfer it to a clean dish.
But the fact that bacteria are growing means the agar works.

Quote:

madis503 said:
and can i re melt the agar what was left over?




If it's clean and not growing anything, yes.


--------------------
You make it all possible. Doesn't it feel good?

Extras: Filter Print Post Top
Offlinemadis503
psühhonaut


Registered: 07/29/09
Posts: 466
Loc: Flag
Last seen: 2 years, 30 days
Re: MEYA problem [Re: Doc_T]
    #16340272 - 06/06/12 09:13 AM (12 years, 7 months ago)

thank you


--------------------

Psy&Goa

Extras: Filter Print Post Top
Offlinemadis503
psühhonaut

Registered: 07/29/09
Posts: 466
Loc: Flag
Last seen: 2 years, 30 days
Re: MEYA problem [Re: madis503]
    #24256659 - 04/19/17 09:23 AM (7 years, 8 months ago)

that recipe has worked for a long time, but about a year i'm having growth problems on this.
is it possbible that the maltose is old?
i tried potato flake agar also, didn't grow anything, even bacteria were slow..
maybe it's the additives in the potato flakes?
also maybe the yeast moisture additive (E491) is bad?
Damn i dont know what to look at anymore..


--------------------

Psy&Goa

Extras: Filter Print Post Top
InvisiblebodhisattaMDiscordReddit
Smurf real estate agent
 User Gallery
Folding@home Statistics
Registered: 04/30/13
Posts: 61,915
Loc: Milky way
Trusted Cultivator
Re: MEYA problem [Re: madis503]
    #24256679 - 04/19/17 09:29 AM (7 years, 8 months ago)

That stuff doesn't really go bad. It just absorbs moisture and gets clumps

Judging by the last four pictures in the first post your problems are coming from being not so good with agar

Extras: Filter Print Post Top
Offlinemadis503
psühhonaut

Registered: 07/29/09
Posts: 466
Loc: Flag
Last seen: 2 years, 30 days
Re: MEYA problem [Re: bodhisatta]
    #24256716 - 04/19/17 09:43 AM (7 years, 8 months ago)

yeah that was 4 years ago...
i've tried with powder maltodextrin also, but it's the wrong stuff i guess, is maltodextrose ok? i think i can get it now from a shop...
i'm just boiling a new bottle of the original formula I used, what used to grow nice myc.
Hoping it will work... really want to make some slants.


--------------------

Psy&Goa

Extras: Filter Print Post Top
InvisiblebodhisattaMDiscordReddit
Smurf real estate agent
 User Gallery
Folding@home Statistics
Registered: 04/30/13
Posts: 61,915
Loc: Milky way
Trusted Cultivator
Re: MEYA problem [Re: madis503]
    #24256722 - 04/19/17 09:45 AM (7 years, 8 months ago)

Maltodextrine is maltodextrose

Extras: Filter Print Post Top
Offlinemadis503
psühhonaut

Registered: 07/29/09
Posts: 466
Loc: Flag
Last seen: 2 years, 30 days
Re: MEYA problem [Re: bodhisatta]
    #24256945 - 04/19/17 10:54 AM (7 years, 8 months ago)

strange, it didn't work good.
Is it possible to overcook the mixture somehow?


--------------------

Psy&Goa

Extras: Filter Print Post Top
InvisiblebodhisattaMDiscordReddit
Smurf real estate agent
 User Gallery
Folding@home Statistics
Registered: 04/30/13
Posts: 61,915
Loc: Milky way
Trusted Cultivator
Re: MEYA problem [Re: madis503]
    #24257083 - 04/19/17 11:41 AM (7 years, 8 months ago)

Yes, don't go much more than 25-30m at 15psi. It only takes 15m to sterilize loads under 1L in volume for most of the basic agar media

Extras: Filter Print Post Top
Offlinemadis503
psühhonaut

Registered: 07/29/09
Posts: 466
Loc: Flag
Last seen: 2 years, 30 days
Re: MEYA problem [Re: bodhisatta]
    #24257232 - 04/19/17 12:41 PM (7 years, 8 months ago)

Quote:

bodhisatta said:
Yes, don't go much more than 25-30m at 15psi. It only takes 15m to sterilize loads under 1L in volume for most of the basic agar media




yeah that might be the problem, i watched LGM videos and they suggested to pc for 1.5 hours i think, if i remember right. so the last time it cooked long... but that doesn't explain the failures before, i trasnfered wedges from older dish and it just wouldn't grow
Anyway I'll comment when i see growth on my new dishes.


--------------------

Psy&Goa

Edited by madis503 (04/19/17 12:43 PM)

Extras: Filter Print Post Top
InvisiblebodhisattaMDiscordReddit
Smurf real estate agent
 User Gallery
Folding@home Statistics
Registered: 04/30/13
Posts: 61,915
Loc: Milky way
Trusted Cultivator
Re: MEYA problem [Re: madis503]
    #24257498 - 04/19/17 02:35 PM (7 years, 8 months ago)

1.5 hours is for grain jars. but I do 2 hours

Extras: Filter Print Post Top
Offlinemadis503
psühhonaut


Registered: 07/29/09
Posts: 466
Loc: Flag
Last seen: 2 years, 30 days
Re: MEYA problem [Re: bodhisatta]
    #24277723 - 04/28/17 02:19 AM (7 years, 8 months ago)

Quote:

bodhisatta said:
1.5 hours is for grain jars. but I do 2 hours



yes i know that also, but thank you for answering.
Anyway, i guess the problem is i overcooked the mix..
But the new batch is good, I put some colonized grain on it, grows well and already made some transfers. :smile:


--------------------

Psy&Goa

Extras: Filter Print Post Top
Jump to top Pages: 1

Shop: OlympusMyco.com No Unicorns Here—Just Quality Bags That Work   North Spore Injection Grain Bag   Left Coast Kratom Buy Kratom Extract   MagicBag.co All-In-One Bags That Don't Suck   Original Sensible Seeds Bulk Cannabis Seeds   PhytoExtractum Maeng Da Thai Kratom Leaf Powder


Similar ThreadsPosterViewsRepliesLast post
* black pin mold on peroxidated MEYA YidakiMan 2,858 12 09/15/03 09:41 PM
by YidakiMan
* Pasteurizing CVG? and problems with bacterial contamination (no pics) Kalypto 1,127 5 05/05/21 08:46 AM
by Kalypto
* LC - maltodextrin? shazoom 1,723 3 04/12/06 10:49 AM
by blackout
* Maltodextrin for LC? StopGo 3,175 6 02/10/19 02:46 PM
by BeefSupremeJr
* problems with green mold on casings jason890 2,404 8 03/28/02 11:24 AM
by Under_net
* premade petri dishes? High_On_Magic 1,984 4 09/15/03 04:10 PM
by simplemachine
* Problems with Agar Innoculation? sparafucile 1,655 3 02/17/02 09:48 AM
by kayaman9
* Steralizing Plastic Petri Dishes
( 1 2 all )
sparafucile 10,341 37 07/13/10 05:19 PM
by Mephistophelian

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, LogicaL Chaos, 13shrooms, hamloaf, cronicr, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a
769 topic views. 27 members, 100 guests and 21 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2025 Mind Media. Some rights reserved.

Generated in 0.028 seconds spending 0.008 seconds on 12 queries.