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Amazon Shop for: ½ Pint Jars, Agar, Brown Rice Flour, Impulse Sealer, Peat, Petri Dish

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InvisibleQuebecShroomer
Shroom Hunter

Registered: 03/16/03
Posts: 41
Loc: In a BIG Amanita
Liquid Mycelium Culture
    #1633825 - 06/14/03 10:20 PM (13 years, 5 months ago)

I have Malabar mycelium growing in a water/malt solution.
Last week I took 10ml of an healthy colonized malt solution to
inoculate 2 quart jars of popcorn substrate.Popcorn is now completly
covered by heavy ryzomorphiq mycelium.I've shake them well and wait for tomorow
to inoculate straw.

I'd like to know what else can I do with this malt solution?
I know I could do some PF cake,but this is not my goal.I want to
try something new!!!

Any idea is greatly appreciated.


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Invisiblemicro
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Registered: 05/09/03
Posts: 7,532
Loc: Brick City
Re: Liquid Mycelium Culture [Re: QuebecShroomer]
    #1633855 - 06/14/03 10:41 PM (13 years, 5 months ago)

Whatever you want to do with live fungal cells :wink:

Maybe inoculate bags with 4 quarts of substrate each bag?

--
Micro


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(Avatar is Maxxy, a character by Mizzyam, RIP)


Edited by micro (06/14/03 10:47 PM)


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InvisibleQuebecShroomer
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Registered: 03/16/03
Posts: 41
Loc: In a BIG Amanita
Re: Liquid Mycelium Culture [Re: micro]
    #1634854 - 06/15/03 12:30 PM (13 years, 5 months ago)

Once straw in a bag is colonized, do you crumble it in a container?
I personally prefer to put the pasteurized straw directly in tha container with
colonized grain and wait for complete colonisation of the substrate.I then apply
a 50/50+ casing layer.

I'd like to have a link or something about straw bags.
Thanks


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Invisiblemicro
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Re: Liquid Mycelium Culture [Re: QuebecShroomer]
    #1635024 - 06/15/03 02:19 PM (13 years, 5 months ago)

That's exactly what I do, except I use straight peat (not generally recommended.)

With bags I was talking about sterilized brf substrate or whole-grain brown rice. Shoot up w/ some liquid mycelium.

--
Micro


--------------------
Any research paper or book for free
(Avatar is Maxxy, a character by Mizzyam, RIP)


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InvisibleQuebecShroomer
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Registered: 03/16/03
Posts: 41
Loc: In a BIG Amanita
Re: Liquid Mycelium Culture [Re: micro]
    #1638676 - 06/17/03 12:42 AM (13 years, 5 months ago)

do you sterilize grain in a bag??I never tried this.
i don't trust putting plastic in my pc.
I like jars cuz I can give some gaz exchanges,easyer to shake and i never saw
any contamination running in these jars.

I thought about using 1 or 2 mycelium syringes to inoculate pasteurized straw.
What you guy thinks!


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Invisiblemicro
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Re: Liquid Mycelium Culture [Re: QuebecShroomer]
    #1638689 - 06/17/03 12:54 AM (13 years, 5 months ago)

I buy autoclavable (pressure-cook-able) bags with filter-patches already on them for air exchange. They work great, but I also have an impulse sealer, now, which makes sealing them a lot easier than when I had to use a lighter.

Zeronio mentioned using tape, however; apparently this can work, too.

I like bags because you can put a lot of substrate in them and shake them (even with brf) so they colonize faster.

--
Micro


--------------------
Any research paper or book for free
(Avatar is Maxxy, a character by Mizzyam, RIP)


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InvisibleQuebecShroomer
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Registered: 03/16/03
Posts: 41
Loc: In a BIG Amanita
Re: Liquid Mycelium Culture [Re: micro]
    #1638721 - 06/17/03 01:12 AM (13 years, 5 months ago)

Thanks for the advice, I'll give it a try.

I've order petri dishes to try strain isolation on agar.
I'm pretty new to agar,so i'd like to know why it is good to
isolate a strain on it.

In my thought, it's like cloning some weed.

Do an isolated strain produce higher yields,bigger fruits,identic mushrooms,
or what!!!
Thanks again for your help micro


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Amazon Shop for: ½ Pint Jars, Agar, Brown Rice Flour, Impulse Sealer, Peat, Petri Dish

Mushrooms, Mycology and Psychedelics >> Advanced Mycology

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