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Offlinec_mathimatics
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Registered: 05/23/03
Posts: 59
Last seen: 14 years, 9 months
Cold Death to Mycelium
    #1629543 - 06/12/03 07:58 PM (15 years, 15 days ago)

Quick Questions:

How long does it take for Mycelium to die at 20 degrees F?

What is the lowest temperature Mycelium can survive (even if not growing)?

Thank you in advance!


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Invisiblemicro
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Re: Cold Death to Mycelium [Re: c_mathimatics]
    #1629607 - 06/12/03 08:23 PM (15 years, 15 days ago)

20 F will do a lot of damage, but no clue as to how long to how much. Cold isn't the problem; the crystalline structure of frozen water has some very sharp edges and will lyse (cut) the cells.

--
Micro


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Invisiblecalicyco
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Registered: 05/03/03
Posts: 355
Re: Cold Death to Mycelium [Re: micro]
    #1629614 - 06/12/03 08:27 PM (15 years, 15 days ago)


Mycelium can survive sub zero temps if frozen properly so the water crystals do not rupture the cell walls. This is done with a cryoprotectant such as glycerin.

20 F is ok but the freezing water will rupture the cell walls.


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Invisiblemicro
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Re: Cold Death to Mycelium [Re: calicyco]
    #1629634 - 06/12/03 08:36 PM (15 years, 15 days ago)

Dimethyl sulfoxide works better.

--
Micro


--------------------
Any research paper or book for free
(Avatar is Maxxy, a character by Mizzyam, RIP)


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OnlineBaby_Hitler
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Re: Cold Death to Mycelium [Re: micro]
    #1629652 - 06/12/03 08:45 PM (15 years, 15 days ago)

Where do you get that?

I can get glycerin at Wal-Mart.


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Invisiblemicro
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Re: Cold Death to Mycelium [Re: Baby_Hitler]
    #1629933 - 06/12/03 10:31 PM (15 years, 15 days ago)

I think they sell it or used to sell it at health food stores as a remedy for muscle cramps, but I work in a lab, so we have a ton of it handy.

You want to know the concentration, though, so you can make up a solution.

--
Micro


--------------------
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(Avatar is Maxxy, a character by Mizzyam, RIP)


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