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OfflineFungalSun
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First time with BRF cakes.. questions
    #16289532 - 05/26/12 08:34 PM (11 years, 11 months ago)

Okay. I'm hitting a tough spot here. I need some advice/troubleshooting, and some help with what to do next. Any help will be greatly! appreciated.

So I inoculated 20 widemouth halfpint BRF jars total. I used a glove box. No pressure cooker for sterilization, but I used a large steamer pot with a tight lid at 120 minutes. The first 10 were inoculated with Golden Teacher from a trusted vendor. The second 10 with B+ from the same trusted vendor.

It's been 28 days since Inoc for the G.T. and 25 days since Inoc for the b+. No foil. They have lids with 4 holes and a dry verm layer. The mix is not packed tightly at all. The two strains are in separate shoeboxes on high shelves at room temp.

The GT has 4 of 10 jars with growth, 2 about half colonized and 2 about a quarter colonized. Growth has slowed down considerably. The other 6 of GT show NO growth. No signs of contamination.

The b+ have shown no growth in any jars. There is a funky smell but it could be the box they're in.. I can't tell.

I don't THINK my mix was too wet, but I suppose it's a possibility.
One thing is that while inoc my GT, I forgot to shake my syringe to distribute the spores in the solution until I had already knocked up a few(ARRRG!!)

So my first question is.. Assuming that oversaturation is not the problem, what else could it be? Not shaking the syringe could explain why only 4 of 10 GT took off, but is that for sure? And what about the B+.? I did shake that one.

My second question is.. what do I do if the jars showing growth don't continue to fully colonize?

Thirdly, if the jars colonize at different rates, do I birth them all at the same time, waiting for each to consolidate, or can I birth them separately and place in my fruiting chamber? (I have 2 fruiting chambers).

Lastly.. I just got two more 10cc syringes. Another b+ and and chitwan. I also have 14 unused jars and plenty of everything else I need to make new jars.

SO.. should I just start two new batches?
Should I sit on these uncolonized jars and wait longer?
I'm also going to make an LC jar.. should I use 1cc from a new syringe to inoc it, or can I use a piece of colonized BRF from one of my current jars?

I realize that is a lot of info and questions.. sorry. Despite the fact that I've had a low success rate so far, I'm still excited and the process has been quite enjoyable for me so far, and I'm only looking forward to learning more and getting better!


--------------------
"You are an explorer, and you represent our species, and the greatest good you can do is to bring back a new idea, because our world is endangered by the absence of good ideas. Our world is in crisis because of the absence of consciousness."
-Terence McKenna

Wild Mushroom Journal

Edited by FungalSun (05/26/12 09:03 PM)

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InvisibleVaeVictum
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Re: First time with BRF cakes.. questions [Re: FungalSun]
    #16289537 - 05/26/12 08:37 PM (11 years, 11 months ago)

Remove the vendors name from your post please. The vendors don't like it when you admit to using spore syringes that they intend for microscopy use only to actually cultivate illegal mushrooms.

VV

EDIT: If you have them enclosed in a box or something this potentially could of stalled them out by forcing them to breathe in the same old air day after day. Not shaking up your syringes probably hurt you too as you already realize.

Yes you can birth the jars as they are reaching 100% and then consolidating for another week. I'd just wait until there's a nice pin set in each jar before birthing it though. They really hit the FC running this way.

I'd start up a new batch right now if you can. If you have the supplies you might as well be using them, right?

Oh and LC made from MS is pretty overrated. Sure you've got this big bottle that you can pull from anytime but in the end you might as well put your time and resources towards learning how to work with agar and isolate a strain then make a master slant.





Edited by VaeVictum (05/26/12 08:43 PM)

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OfflineFungalSun
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Re: First time with BRF cakes.. questions [Re: VaeVictum]
    #16289571 - 05/26/12 08:46 PM (11 years, 11 months ago)

Hmm.. I thought I edited the vendors name out before I submitted the post and it's not showing it now. You obviously saw it though.. weird.

Do you think that removing them from the boxes will help at this point?


--------------------
"You are an explorer, and you represent our species, and the greatest good you can do is to bring back a new idea, because our world is endangered by the absence of good ideas. Our world is in crisis because of the absence of consciousness."
-Terence McKenna

Wild Mushroom Journal

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InvisibleVaeVictum
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Re: First time with BRF cakes.. questions [Re: FungalSun]
    #16289586 - 05/26/12 08:50 PM (11 years, 11 months ago)

It's hard to say really. Don't be afraid to leave your colonizing Pf Tek jars sitting on a book shelf, dresser, entertainment center or whatever. They are designed to filter out contaminates so taking extra precaution by doing other things to "help" them usually ends up being detrimental to the grow.

If you have jars that aren't even 50% done by 20+ days I'd go ahead and chuck 'em and start all over again.

VV

P.S. Be sure to shake your syringes this time homeslice. :awesomenod:

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OfflineMugnath
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Re: First time with BRF cakes.. questions [Re: VaeVictum]
    #16289622 - 05/26/12 08:57 PM (11 years, 11 months ago)

Third paragraph fom the bottom, it says where you received the second supply of four 10cc syringes, edit that out. I also agree with Vae about the boxes. Go ahead and remove them, also what exactly is room temperature?

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OfflineFungalSun
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Re: First time with BRF cakes.. questions [Re: VaeVictum]
    #16289670 - 05/26/12 09:07 PM (11 years, 11 months ago)

Quote:

VaeVictum said:
P.S. Be sure to shake your syringes this time homeslice. :awesomenod:




Yeah.. I think that was what killed me on my first 10.. but I shook the second syringe for the next 10 and none of them took off.. so is the box what got me with those you think?


--------------------
"You are an explorer, and you represent our species, and the greatest good you can do is to bring back a new idea, because our world is endangered by the absence of good ideas. Our world is in crisis because of the absence of consciousness."
-Terence McKenna

Wild Mushroom Journal

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OfflineFungalSun
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Re: First time with BRF cakes.. questions [Re: Mugnath]
    #16289684 - 05/26/12 09:10 PM (11 years, 11 months ago)

Quote:

Mugnath said:
Third paragraph fom the bottom, it says where you received the second supply of four 10cc syringes, edit that out. I also agree with Vae about the boxes. Go ahead and remove them, also what exactly is room temperature?




Ahh missed that one.. thanks. And room temp is variable.. between 60 and 70. Spring in Portland, OR usually doesn't provide steady temps unfortunately.


--------------------
"You are an explorer, and you represent our species, and the greatest good you can do is to bring back a new idea, because our world is endangered by the absence of good ideas. Our world is in crisis because of the absence of consciousness."
-Terence McKenna

Wild Mushroom Journal

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InvisibleVaeVictum
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Registered: 06/01/11
Posts: 4,397
Re: First time with BRF cakes.. questions [Re: FungalSun]
    #16289703 - 05/26/12 09:14 PM (11 years, 11 months ago)

It's possible that they were starved of gas exchange and stalled out. It could of been your moisture content too, how wet do your jars look? Do you have any pictures by chance? Your temps are fine for colonizing and fruiting.


If you live in Portland though why not head over to the coast when the time is right and pick as many azures as you can find and be set on psychedelics for a long while.

VV

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OfflineFungalSun
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Re: First time with BRF cakes.. questions [Re: VaeVictum]
    #16289777 - 05/26/12 09:31 PM (11 years, 11 months ago)

VaeVictum said:
It's possible that they were starved of gas exchange and stalled out. It could of been your moisture content too, how wet do your jars look? Do you have any pictures by chance? Your temps are fine for colonizing and fruiting.


If you live in Portland though why not head over to the coast when the time is right and pick as many azures as you can find and be set on psychedelics for a long while.

VV




I do that too :grin:.. but I've wanted to learn this for a long time too.

Here's two pics.. it's hard to see unless you click on the images, but the colonizing jar has small drops of condensation inside. The un-colonized jar has no condensation or signs of excess moisture.



--------------------
"You are an explorer, and you represent our species, and the greatest good you can do is to bring back a new idea, because our world is endangered by the absence of good ideas. Our world is in crisis because of the absence of consciousness."
-Terence McKenna

Wild Mushroom Journal

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OfflineElftastic
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Re: First time with BRF cakes.. questions [Re: FungalSun]
    #16289809 - 05/26/12 09:36 PM (11 years, 11 months ago)

Condensation just indicates a difference in temperature, and is normal. That second jar looks like it's slowly chugging along.


--------------------

:discorex: :nyan: :nyan:"Yes, this is Dog." - Dog :nyan: :nyan: :discorex:

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InvisibleVaeVictum
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Re: First time with BRF cakes.. questions [Re: Elftastic]
    #16289841 - 05/26/12 09:44 PM (11 years, 11 months ago)

Your jars look pretty good. Keep us posted on your grow.

VV

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OfflineFungalSun
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Re: First time with BRF cakes.. questions [Re: VaeVictum]
    #16290218 - 05/26/12 10:48 PM (11 years, 11 months ago)

Okay so I've got them all out of the boxes now.. hopefully at least some of them get a chance to shine.

I also just checked out my B+ which had no colonization a few days ago and now 3 of them are showing a little growth.

So seeing that it's been 28 and 25 days since inoc I'm not sure if they will pull through. Time will tell.

So is agar is the way to go over LC if I'm looking to keep doing this?
I've done some research on both but not enough to understand the pros and cons of each.


--------------------
"You are an explorer, and you represent our species, and the greatest good you can do is to bring back a new idea, because our world is endangered by the absence of good ideas. Our world is in crisis because of the absence of consciousness."
-Terence McKenna

Wild Mushroom Journal

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InvisibleVaeVictum
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Registered: 06/01/11
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Re: First time with BRF cakes.. questions [Re: FungalSun]
    #16290227 - 05/26/12 10:50 PM (11 years, 11 months ago)

Agar will allow you to do isolates, clones and progress in this hobby a lot more. If you make an LC from an MS syringe you'll just have a ton of shitty solution to make into syringes.

VV

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OfflineFungalSun
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Re: First time with BRF cakes.. questions [Re: VaeVictum]
    #16290482 - 05/27/12 12:08 AM (11 years, 11 months ago)

Sounds good.. thanks for the help.. it is sooo much appreciated. I LOVE this forum and everyone on it that are so helpful to people just starting out.

I'll do some research on agar soon.
Right now I'm just getting pumped to go see Beck tomorrow. Life is great.


--------------------
"You are an explorer, and you represent our species, and the greatest good you can do is to bring back a new idea, because our world is endangered by the absence of good ideas. Our world is in crisis because of the absence of consciousness."
-Terence McKenna

Wild Mushroom Journal

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OfflineMugnath
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Re: First time with BRF cakes.. questions [Re: FungalSun]
    #16290863 - 05/27/12 02:59 AM (11 years, 11 months ago)

Lucky, please post pics on here if those jars do pull through, I'm very curious.

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OfflineFungalSun
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Re: First time with BRF cakes.. questions [Re: Mugnath]
    #16291732 - 05/27/12 10:29 AM (11 years, 11 months ago)

Will do.


--------------------
"You are an explorer, and you represent our species, and the greatest good you can do is to bring back a new idea, because our world is endangered by the absence of good ideas. Our world is in crisis because of the absence of consciousness."
-Terence McKenna

Wild Mushroom Journal

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InvisibleVaeVictum
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Re: First time with BRF cakes.. questions [Re: FungalSun]
    #16291766 - 05/27/12 10:37 AM (11 years, 11 months ago)

Quote:

FungalSun said:
Sounds good.. thanks for the help.. it is sooo much appreciated. I LOVE this forum and everyone on it that are so helpful to people just starting out.




Hey man, that's what a lot of us are here for. While I still grow here and there I enjoy helping shroomery members more than anything.

Have you started anymore jars yet though? I know you said you had a bunch of empty ones, just wondering if you've started any work on that yet.

BTW I don't think I mentioned this specifically but your BRF jars are text book examples of how the substrate inside should look when you combine everything thoroughly and load the jars gently.

Keep up the great work!

VV

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OfflineChilled
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Re: First time with BRF cakes.. questions [Re: VaeVictum]
    #16293478 - 05/28/12 01:05 AM (11 years, 11 months ago)

Hi there!

I am also on my first grow (check out my signature to see, has lots of photos to follow the process) and I have had 4/8 show growth.

Here my top tips for my grow so far:

1. Let the jar cool overnight!! I believe that as a stacked my jars 4-4 in my pressure cooker the bottom 4 were still too hot and killed the spores when I injected them. I only left them to cool for a few hours when really they need 24hr+. If this was the reason for mine, had a waited I would have got 100% success rate!

2. Work out your ratios with some maths! Work out the total volume of all the jars you will be filling, add 10% for waste/inaccuracy and then divide by four for the 2:1:1 ratio of verm:water:BRF. This way you are sure not to have your jar too dry/wet.

3. Leave your jars in a box next to a radiator. This means that your jars are slightly warmer than room temp making the grow increase.

4. Try do less next time. I did 8 268ml jars so that I could make sure I mastered a small batch before up scaling. It also means, if they all go tits up, you have wasted very little and 8 268ml jars can yield up to 2oz dry which I think is more than enough for a first grow (although I only have 4 that have growth now!). To be fair, both the jars you have posted up look similar to mine, which seem to be on track (maybe a little behind). 

RE: Birthing I think you should only birth each when they have 100% colonised AND had a week to consolidate. If that means you have lots of different stages in your fruiting chamber then so be it.

And 100%, this site is fantastic and I have learnt so much already!

Edited by Chilled (05/28/12 01:07 AM)

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OfflineFungalSun
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Re: First time with BRF cakes.. questions [Re: VaeVictum]
    #16295969 - 05/28/12 03:13 PM (11 years, 11 months ago)

Quote:

VaeVictum said:
Have you started anymore jars yet though?





Haven't got a chance to yet.. still recovering from an awesome concert lastnight. I may start tonight if I feel up to it.

Quote:

VaeVictum said:
BTW I don't think I mentioned this specifically but your BRF jars are text book examples of how the substrate inside should look when you combine everything thoroughly and load the jars gently.

Keep up the great work!






Thank you! That's pretty good to hear because I wasn't 100% sure about the moisture content of the mix till now. I'll keep you updated on the whole shebang as the days go.


--------------------
"You are an explorer, and you represent our species, and the greatest good you can do is to bring back a new idea, because our world is endangered by the absence of good ideas. Our world is in crisis because of the absence of consciousness."
-Terence McKenna

Wild Mushroom Journal

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OfflineFungalSun
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Re: First time with BRF cakes.. questions [Re: FungalSun]
    #16298391 - 05/28/12 10:43 PM (11 years, 11 months ago)

16 on the stovetop!


--------------------
"You are an explorer, and you represent our species, and the greatest good you can do is to bring back a new idea, because our world is endangered by the absence of good ideas. Our world is in crisis because of the absence of consciousness."
-Terence McKenna

Wild Mushroom Journal

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Shop: Mushroom-Hut Liquid Cultures   PhytoExtractum Kratom Powder for Sale   Original Sensible Seeds Bulk Cannabis Seeds   North Spore Bulk Substrate   Myyco.com Golden Teacher Liquid Culture For Sale   MagicBag.co Certified Organic All-In-One Grow Bags   Left Coast Kratom Buy Kratom Capsules   OlympusMyco.com Olympus Myco Bulk Substrate   Kraken Kratom Red Vein Kratom


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