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skuzzy1232
:))



Registered: 05/31/10
Posts: 1,460
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Re: could it be ? yes it can 30TUB GROW!!!!!!!!! [Re: skuzzy1232]
#16674059 - 08/09/12 10:35 PM (12 years, 5 months ago) |
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nm i was just educated
-------------------- http://www.shroomery.org/forums/showflat.php/Cat/0/Number/8468463
Have any questions comments PM me ill try to help out the only stupid question is the one you dont ask .
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Softpaw
Who knows...It MAY work



Registered: 06/27/12
Posts: 811
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Re: could it be ? yes it can 30TUB GROW!!!!!!!!! [Re: skuzzy1232]
#16674086 - 08/09/12 10:40 PM (12 years, 5 months ago) |
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I'm still confused. HElp me out please.
Where is my logic mixed up here.
If you have a tub of B+ and get a cap from a mushie, dry it, get the prints, and put the print into a syringe, how do those spores have the possibility to have PE, EQ, or whatever if they came from a B+ cap??
What the heck am I missing?
Also, if I did the same with a tissue sample of B+ isn't the strain the same as if I did it in the above fashion? I mean, the spores came from the same mushroom.
So,if I tissue sampled a mushie and took spores from the same mushie, I'm still getting thousands of strains from the spores but a single strain from the tissue??
Grrrrr. I can't grasp this concept for some reason. Starting to make me a littel impatient.
-------------------- "We're the middle children of history, man. No purpose or place. We have no Great War. No Great Depression. Our Great War's a spiritual war... our Great Depression is our lives. We've all been raised on television to believe that one day we'd all be millionaires, and movie gods, and rock stars. But we won't. And we're slowly learning that fact. And we're very, very pissed off."
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k00laid
NEMO


Registered: 05/03/10
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Re: could it be ? yes it can 30TUB GROW!!!!!!!!! [Re: Softpaw]
#16674103 - 08/09/12 10:44 PM (12 years, 5 months ago) |
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well it gets more complicated, 
if you clone a fruit, there are even possibilities of that containing more than 1 strain.
the only way to check if you have a "strain" is to grow it on agar and see that it is a "monoculture"
meaning NO sectoring. completely uniform. genetically identical mycelium.
softpaw, think of cubes as humans, if a peurto rican fucks another, theyll have a little PR baby, and if two white people go at it, they'll have a white baby
but sometimes albino's come out of nowhere.
sometimes a recessive gene surfaces and someone looks darker, or lighter.
its just random genetics.
the only thing is, mushrooms do it by themselves.
so if you have a PR mushroom, most likely everything that grows from the spores will look generally like the original PR you printed from.
but not always.
-------------------- AMU - AMU Q & A - MyVideo Teks!
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skuzzy1232
:))



Registered: 05/31/10
Posts: 1,460
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Re: could it be ? yes it can 30TUB GROW!!!!!!!!! [Re: Softpaw]
#16674124 - 08/09/12 10:48 PM (12 years, 5 months ago) |
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ok if you have x amount of spores you have thousands of strains ok ?
now PE red spore etc are psilocyben strains , BUT are different variety's of the same strain you digg ?? the reason all the all the shrooms look and act the same PE = PE mushies is because the spores inbreed giving similar characteristics , but you cant mix two variety's together without a lab or some snake venom to break down there cell walls as was the case with RR and hi PE6 hybrid.
-------------------- http://www.shroomery.org/forums/showflat.php/Cat/0/Number/8468463
Have any questions comments PM me ill try to help out the only stupid question is the one you dont ask .
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Softpaw
Who knows...It MAY work



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Re: could it be ? yes it can 30TUB GROW!!!!!!!!! [Re: k00laid]
#16674128 - 08/09/12 10:49 PM (12 years, 5 months ago) |
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Ahhh. That makes sense now.. Thank you..
So, why then do spores make that chance happen more often than tissue? Just becuase there are more spores thus meaning more possibility? I think I'm starting to understand. So, mushrooms are asexual then? I know, stupid question. Even with spores?
-------------------- "We're the middle children of history, man. No purpose or place. We have no Great War. No Great Depression. Our Great War's a spiritual war... our Great Depression is our lives. We've all been raised on television to believe that one day we'd all be millionaires, and movie gods, and rock stars. But we won't. And we're slowly learning that fact. And we're very, very pissed off."
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Softpaw
Who knows...It MAY work



Registered: 06/27/12
Posts: 811
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Last seen: 12 years, 2 months
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Re: could it be ? yes it can 30TUB GROW!!!!!!!!! [Re: skuzzy1232]
#16674143 - 08/09/12 10:52 PM (12 years, 5 months ago) |
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Quote:
skuzzy1232 said: ok if you have x amount of spores you have thousands of strains ok ?
now PE red spore etc are psilocyben strains , BUT are different variety's of the same strain you digg ?? the reason all the all the shrooms look and act the same PE = PE mushies is because the spores inbreed giving similar characteristics , but you cant mix two variety's together without a lab or some snake venom to break down there cell walls as was the case with RR and hi PE6 hybrid.
Okay, I'm getting it. THe last part is going past me, but I'm starting to understand. This is where I was getting confused. I need to read about this crap.
Thanks guys. I appreciate your patience with this a ton. This has been bugging me for a long time. Its a concept that is blowing past me.
-------------------- "We're the middle children of history, man. No purpose or place. We have no Great War. No Great Depression. Our Great War's a spiritual war... our Great Depression is our lives. We've all been raised on television to believe that one day we'd all be millionaires, and movie gods, and rock stars. But we won't. And we're slowly learning that fact. And we're very, very pissed off."
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skuzzy1232
:))



Registered: 05/31/10
Posts: 1,460
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Re: could it be ? yes it can 30TUB GROW!!!!!!!!! [Re: Softpaw]
#16674149 - 08/09/12 10:53 PM (12 years, 5 months ago) |
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as far as i know spores just share genetic material when there being made and formed ....they don't actually reproduce i could be wrong.
-------------------- http://www.shroomery.org/forums/showflat.php/Cat/0/Number/8468463
Have any questions comments PM me ill try to help out the only stupid question is the one you dont ask .
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Softpaw
Who knows...It MAY work



Registered: 06/27/12
Posts: 811
Loc: Sconnie
Last seen: 12 years, 2 months
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Re: could it be ? yes it can 30TUB GROW!!!!!!!!! [Re: skuzzy1232]
#16674167 - 08/09/12 10:56 PM (12 years, 5 months ago) |
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Its such a cool topic. I think this is fascinating actually. I would love to see what RR has said about this. I wish I would have joined this community years ago so I have been caught up on the research of this type of thing.
I'm a huge research fan (And I'm not talking about people saying they have to research something so they go to their google machines). and would love to look into this further. I have one of the US's largest top 10 libraries to my disposal and would love to utilize it. I just need to focus in a little further with this concept.
Can you brief me what RR did with PE-6?
-------------------- "We're the middle children of history, man. No purpose or place. We have no Great War. No Great Depression. Our Great War's a spiritual war... our Great Depression is our lives. We've all been raised on television to believe that one day we'd all be millionaires, and movie gods, and rock stars. But we won't. And we're slowly learning that fact. And we're very, very pissed off."
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wildernessjunkie
Reshitivest



Registered: 06/13/10
Posts: 8,118
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Re: could it be ? yes it can 30TUB GROW!!!!!!!!! [Re: skuzzy1232]
#16674180 - 08/09/12 10:58 PM (12 years, 5 months ago) |
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It takes two spores to reproduce.
I always thought of "strains" more as stabilized genetics. But the true definition of strain is a mono- culture. What the vendor labels the syringe as does not change the fact that they are all Cubensis. You can shoot GT and Red boy in a jar, and still get cubes from it. Some spores would be purple and some red, but still the same mushroom.
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Softpaw
Who knows...It MAY work



Registered: 06/27/12
Posts: 811
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Last seen: 12 years, 2 months
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Re: could it be ? yes it can 30TUB GROW!!!!!!!!! [Re: wildernessjunkie]
#16674186 - 08/09/12 11:00 PM (12 years, 5 months ago) |
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^^^ that makes sense now...
This is a little frustrating if you want consistency then isn't it?
-------------------- "We're the middle children of history, man. No purpose or place. We have no Great War. No Great Depression. Our Great War's a spiritual war... our Great Depression is our lives. We've all been raised on television to believe that one day we'd all be millionaires, and movie gods, and rock stars. But we won't. And we're slowly learning that fact. And we're very, very pissed off."
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skuzzy1232
:))



Registered: 05/31/10
Posts: 1,460
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Re: could it be ? yes it can 30TUB GROW!!!!!!!!! [Re: Softpaw]
#16674187 - 08/09/12 11:00 PM (12 years, 5 months ago) |
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he cross strained PE and texas together using rattle snake venom to break down cell walls making the hybrid as far as i know its PE that drops texas spores ..or something like that if you want your mind blow click RR's notes in my sig
-------------------- http://www.shroomery.org/forums/showflat.php/Cat/0/Number/8468463
Have any questions comments PM me ill try to help out the only stupid question is the one you dont ask .
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skuzzy1232
:))



Registered: 05/31/10
Posts: 1,460
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Re: could it be ? yes it can 30TUB GROW!!!!!!!!! [Re: skuzzy1232]
#16674193 - 08/09/12 11:02 PM (12 years, 5 months ago) |
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-------------------- http://www.shroomery.org/forums/showflat.php/Cat/0/Number/8468463
Have any questions comments PM me ill try to help out the only stupid question is the one you dont ask .
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k00laid
NEMO


Registered: 05/03/10
Posts: 19,636
Last seen: 1 year, 5 months
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Re: could it be ? yes it can 30TUB GROW!!!!!!!!! [Re: skuzzy1232]
#16674205 - 08/09/12 11:04 PM (12 years, 5 months ago) |
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no no no
sorry
the snake venom was only because the spores were old and things.
you CAN mix PE spores with B+ spores and you might get PE looking fruits or you might get B+ looking fruits, or maybe a mix of both.
because they are both spores of the same species of mushroom.
they will fruit together.
thats why PE is a variety
genus species variety
psilocybe cubensis penis envy
-------------------- AMU - AMU Q & A - MyVideo Teks!
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k00laid
NEMO


Registered: 05/03/10
Posts: 19,636
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Re: could it be ? yes it can 30TUB GROW!!!!!!!!! [Re: k00laid]
#16674209 - 08/09/12 11:05 PM (12 years, 5 months ago) |
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oh and to make this whole topic even more confusing and shit.
StoneSun says isolating strains from cubensis is a waste of time.
they are so inbred that multispore is perfect enough for him.
but i'm just talking about textbook definitions of what a strain really is in mycological terms.
-------------------- AMU - AMU Q & A - MyVideo Teks!
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wildernessjunkie
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Re: could it be ? yes it can 30TUB GROW!!!!!!!!! [Re: skuzzy1232]
#16674211 - 08/09/12 11:06 PM (12 years, 5 months ago) |
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If I recall, RR did that with RedBoy and Texas. But the venom was only necessary because he could not get the red spores to germinate due to age. The venom broke down the cell walls and allowed the DNA to combine without spore germination. Someone correct me if I'm wrong.
I think that PE may be Workman's baby, though I can't swear to it.
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Softpaw
Who knows...It MAY work



Registered: 06/27/12
Posts: 811
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Re: could it be ? yes it can 30TUB GROW!!!!!!!!! [Re: k00laid]
#16674213 - 08/09/12 11:06 PM (12 years, 5 months ago) |
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Whew... I'll read that thread and look into this more. I'm a little overwhelmed and sleepy so my brain is starting to shut down.
-------------------- "We're the middle children of history, man. No purpose or place. We have no Great War. No Great Depression. Our Great War's a spiritual war... our Great Depression is our lives. We've all been raised on television to believe that one day we'd all be millionaires, and movie gods, and rock stars. But we won't. And we're slowly learning that fact. And we're very, very pissed off."
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skuzzy1232
:))



Registered: 05/31/10
Posts: 1,460
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Re: could it be ? yes it can 30TUB GROW!!!!!!!!! [Re: Softpaw]
#16674237 - 08/09/12 11:09 PM (12 years, 5 months ago) |
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before you go RR says Another way to ensure you're getting the genetics you want is to cross two dikaryotic known fruiting strains. It fails about 90% of the time or more, but that's still probably better odds than breeding monokaryons, and then waiting to see if it's a good fruiter with the qualities you desire.
Put the two strains on the same petri dish and let them run together. If a third sector opens up along the line of isolation, it's a third strain. You can test it by placing on a petri dish with the two originals. If they develop a line of isolation between all three, you know you've succeeded. RR
-------------------- http://www.shroomery.org/forums/showflat.php/Cat/0/Number/8468463
Have any questions comments PM me ill try to help out the only stupid question is the one you dont ask .
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Softpaw
Who knows...It MAY work



Registered: 06/27/12
Posts: 811
Loc: Sconnie
Last seen: 12 years, 2 months
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Re: could it be ? yes it can 30TUB GROW!!!!!!!!! [Re: skuzzy1232]
#16674244 - 08/09/12 11:11 PM (12 years, 5 months ago) |
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Mono and DIka..okay I know what those roots are but I need to pull out the dictionary on a few of these terms. Some of them are new to me.
That sounds like something I can understand and a place where I can begin. Thanks for helping me out with this guys.
-------------------- "We're the middle children of history, man. No purpose or place. We have no Great War. No Great Depression. Our Great War's a spiritual war... our Great Depression is our lives. We've all been raised on television to believe that one day we'd all be millionaires, and movie gods, and rock stars. But we won't. And we're slowly learning that fact. And we're very, very pissed off."
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wildernessjunkie
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Re: could it be ? yes it can 30TUB GROW!!!!!!!!! [Re: Softpaw]
#16674277 - 08/09/12 11:21 PM (12 years, 5 months ago) |
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Monokaryon - A single spore that has germinted.
Dikaryon - Two Monokaryon's combining and sharing genetic material for reproduction. Same as a monoculture the way I understand it.
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Softpaw
Who knows...It MAY work



Registered: 06/27/12
Posts: 811
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Re: could it be ? yes it can 30TUB GROW!!!!!!!!! [Re: wildernessjunkie]
#16674329 - 08/09/12 11:36 PM (12 years, 5 months ago) |
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Thank you
-------------------- "We're the middle children of history, man. No purpose or place. We have no Great War. No Great Depression. Our Great War's a spiritual war... our Great Depression is our lives. We've all been raised on television to believe that one day we'd all be millionaires, and movie gods, and rock stars. But we won't. And we're slowly learning that fact. And we're very, very pissed off."
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