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ciround
Stranger

Registered: 05/10/12
Posts: 11
Last seen: 12 years, 7 months
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how long can i keep a sterilized rye grain jar for before innoculating?
#16211027 - 05/10/12 05:13 PM (12 years, 8 months ago) |
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im waiting for my liquid culture to colonize but have sterilized my jars (probably a mistake) can i keep them for any amount of time before the grain drys out or should i start over and with for my myc?
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OH1Ogrown
Student



Registered: 04/23/12
Posts: 1,214
Loc: Treasure Coast
Last seen: 1 year, 8 months
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Re: how long can i keep a sterilized rye grain jar for before innoculating? [Re: ciround]
#16211034 - 05/10/12 05:15 PM (12 years, 8 months ago) |
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I wouldn't wait longer than a few days.
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Diesel_Dawg
Not a noob


Registered: 05/21/09
Posts: 397
Last seen: 12 years, 6 months
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Re: how long can i keep a sterilized rye grain jar for before innoculating? [Re: OH1Ogrown]
#16211436 - 05/10/12 06:42 PM (12 years, 8 months ago) |
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Its not a good idea to leave them sitting around, but if they are sterilized properly, you can get away with letting them wait a little while.
I recently inoculated some rye bags that I let sit for nearly a month and a half and they did just fine, not recommended, but I did get away with it.
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PocketRevolution
Stranger
Registered: 12/22/09
Posts: 270
Last seen: 7 months, 26 days
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Re: how long can i keep a sterilized rye grain jar for before innoculating? [Re: Diesel_Dawg]
#16211487 - 05/10/12 06:49 PM (12 years, 8 months ago) |
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I've let them sit as much as a week with no perceptible ill effects.
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FunguSphere
Apprentice



Registered: 08/11/11
Posts: 127
Last seen: 1 year, 9 months
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Re: how long can i keep a sterilized rye grain jar for before innoculating? [Re: PocketRevolution]
#16211602 - 05/10/12 07:13 PM (12 years, 8 months ago) |
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Yeah the longer you wait the higher the chances of getting a contam. You can get away with it but I don't see the point in risking it.
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"To doubt the literal meaning of the words of Jesus or Moses incurs hostility from most people, but it's just a fact that if Jesus or Moses were to appear today, unidentified, with the same message he spoke many years ago, his mental stability would be challenged." - ZAMM
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EvilMushroom666
Heretic




Registered: 11/18/09
Posts: 10,289
Loc: Canada
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Re: how long can i keep a sterilized rye grain jar for before innoculating? [Re: FunguSphere]
#16211630 - 05/10/12 07:20 PM (12 years, 8 months ago) |
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The rate at which your grain will dry depends upon the material you have used as a filter.
I find with filter discs you can easily let grain sit for 1-1.5 months without much moisture lose(although there 100% is some). Polyfil and tyvek I find tend to allow the grain to lose moisture a little faster, I would not leave jars for much longer then 2-3 weeks with these materials. Bags I find also tend to dry out quicker due to the larger surface area of exposed grain in the plenum of the bag.
If you have sterilized your jars correctly you should not see any contamination at any point, even if you leave them for a few weeks.
If your grain dries up a fair bit you can compensate by adding a bit of extra LC when you inoculate them.
All this being said ideally you want to go ahead and make grain and use it within a day or two for the best results. Sometimes shit happens, and in my case with a busy work schedule and life I tend to make grain ahead of time on my days off, and inoculate later at my leisure.
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