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OfflineCynosure
allow me to be your guide.
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Registered: 10/06/09
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Spores aren't germinating!
    #16205518 - 05/09/12 04:10 PM (12 years, 8 months ago)

Hey there,

I have many spore prints of Hillbilly from two of my prior grows.  I've turned many of them over to syringes.

None of the spores are germinating, though!  I've inoculated jars, liquid cultures, and agar.  There hasn't been growth on any medium from over 7+ syringes of different prints.

Anyone care to chime in on this crazy event?  What's causing it?


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"You can peel it [language] off the ceiling and make it dance in front of you" - McKenna

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OfflineTheBot0nist
Stretching my Cotyledons
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Registered: 03/30/12
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Re: Spores aren't germinating! [Re: Cynosure]
    #16205553 - 05/09/12 04:14 PM (12 years, 8 months ago)

How long have you waited? Did they get really hot, or freeze? Any noticable contams?


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U.T.F.S.E., and learn the joys of autodidacticism!

"Therefore I should infer from analogy that probably all the organic beings which have ever lived on this earth have descended from some one primordial form, into which life was first breathed." - On the Origin of Species Charles Darwin

The Secret of Life. Read it all, and every link, this is not a joke.


Don't judge each day only by the harvests you reap, but also by the seeds you sow.

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OfflineCynosure
allow me to be your guide.
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Registered: 10/06/09
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Last seen: 1 year, 10 months
Re: Spores aren't germinating! [Re: TheBot0nist]
    #16206109 - 05/09/12 06:05 PM (12 years, 8 months ago)

No notable contaminants.  The LC's did eventually contaminate but it wasn't until many weeks later (which is to be expected).  I simply re-inoculated the jars that didn't germinate a few weeks after and the new spores germinated and colonized just fine.

I waited and watched each for about 3 weeks.  I had 4 LC's, 3 agar dishes, and about 12 WBS jars.  I used different syringes on them all (with exceptions of the jars-6 jars, 1 syringe).

They've been stored in the same place as all my other prints/syringes which have done just fine.  It has never gone above 90 or below 40 in here even with the windows open.


--------------------
"You can peel it [language] off the ceiling and make it dance in front of you" - McKenna

<3 .

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