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OfflineOrkidea
Stranger

Registered: 12/28/11
Posts: 53
Last seen: 3 years, 8 months
Question about prints
    #16204206 - 05/09/12 10:43 AM (12 years, 8 months ago)

Hello
Some weeks ago I made 5jars of rye spawn. It looked cool and everyhting  but when jars were colonized 85-90% they started showing bacterial contam.
So I wondering right now. Do you guys think my spore print was bad and it should not be used again or do you think I should give a try with this print again?
Thank you

Edited by Orkidea (05/09/12 10:44 AM)

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InvisibleThadeous
On the path


Registered: 08/02/11
Posts: 1,101
Re: Question about prints [Re: Orkidea]
    #16204234 - 05/09/12 10:54 AM (12 years, 8 months ago)

How did you sterilize and what were your inoculation procedures?

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InvisibleTranscendingLife
I Don't Need a Life to Live
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Registered: 06/09/10
Posts: 21,627
Trusted Cultivator
Re: Question about prints [Re: Thadeous]
    #16204280 - 05/09/12 11:07 AM (12 years, 8 months ago)

It could be the syringe/print or your grains were too wet when you inoculated.


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OfflineLifted1
Space Cadet
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Registered: 03/28/11
Posts: 1,703
Loc: North East USA Flag
Last seen: 11 years, 1 month
Re: Question about prints [Re: TranscendingLife]
    #16204484 - 05/09/12 12:00 PM (12 years, 8 months ago)

And perhaps a bad jar lid design/ bad filter.


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InvisibleIll-bird

Registered: 12/14/11
Posts: 1,782
Loc: Amsterdam
Re: Question about prints [Re: Orkidea]
    #16204525 - 05/09/12 12:14 PM (12 years, 8 months ago)

i agree with tl that it could have been a few things, did you make the print or what? its always a good idea to start a print out on agar first, especially if you didnt make it yourself and dont know how much care someone took to keep the print aseptic.

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