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North Spore Shop: Injection Grain Bag

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OfflineCoupsDeGrace
PsychoMyco
Male
Registered: 04/22/12
Posts: 127
Loc: Appalachia
Last seen: 4 years, 7 months
Contam in Jar?
    #16196292 - 05/07/12 06:37 PM (12 years, 8 months ago)

First attempt ever - Using PF Tek cakes, but substituted the BRF with Dark Rye Flour.  This is about 4 days after inoc. Once the black was first seen, with lingering moisture, the cake was isolated.  ALL images are of the same cake.

A few days later, both the mycel colony and the black grew.  The jar was sanitized for 1.5 hours, then disposed off.  Just curious of what it was and if it's common in the mid eastern U.S.







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:mushroom2: Juan with the shroom :mushroom2:

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OfflineCloset-Mushroom
Psychonaut

Registered: 08/03/10
Posts: 216
Last seen: 11 years, 10 months
Re: Contam in Jar? [Re: CoupsDeGrace]
    #16196796 - 05/07/12 08:18 PM (12 years, 8 months ago)

Typical black mold. Very common, but not as common as trich. Out of curiosity, how long did you PC your jars for? How many jars did you make?

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OfflineCoupsDeGrace
PsychoMyco
Male
Registered: 04/22/12
Posts: 127
Loc: Appalachia
Last seen: 4 years, 7 months
Re: Contam in Jar? [Re: Closet-Mushroom]
    #16197116 - 05/07/12 09:20 PM (12 years, 8 months ago)

Thank you very much for the input!  Guess I shouldn't worry so much if it's just a common problem. 

There were 10 jars to start, and a PC wasn't used.  They were sterilized in a boiling pot of water (1.5 hours) as shown in the PF tek; with a few innovations to sterilize all 10 jars at once. When the contam jar was discovered, it was boiled the same way for 1.5 hours.

With the contam jar exempt, there are 9 potentially healthy cakes!


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:mushroom2: Juan with the shroom :mushroom2:

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OfflineCloset-Mushroom
Psychonaut

Registered: 08/03/10
Posts: 216
Last seen: 11 years, 10 months
Re: Contam in Jar? [Re: CoupsDeGrace]
    #16199133 - 05/08/12 09:55 AM (12 years, 8 months ago)

Good to hear about a 90% success rate thus far. Always a good idea to boil bad jars. Just try to invest in a large enough PC and it seems like you will be all set. I may be wrong, but I think black mold is most commonly a result of an under sterilized jar. (Anyone feel free to correct me if i'm wrong)

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OfflineCoupsDeGrace
PsychoMyco
Male
Registered: 04/22/12
Posts: 127
Loc: Appalachia
Last seen: 4 years, 7 months
Re: Contam in Jar? [Re: Closet-Mushroom]
    #16201316 - 05/08/12 07:20 PM (12 years, 8 months ago)

Could be the jar, but maybe the needle...I didn't sterilize the needle after every jar while inoculating.  Planned it at a bad time (really eager to try it out) and was rushed.  The mold could of resulted from that.


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North Spore Shop: Injection Grain Bag


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