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Offlinekendawg
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contam maybe in my wbs
    #16199549 - 05/08/12 11:55 AM (12 years, 8 months ago)

Hey whats up guys? I am having a hard time  deciding if these 2 wbs jars are bad. I knocked them up with ecuador. Im doing some monos up later today. You guys think these are good or bad. I shook them at 30% colonized. I hope they're good if not shit happens.




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Re: contam maybe in my wbs [Re: kendawg]
    #16199554 - 05/08/12 11:58 AM (12 years, 8 months ago)

Bad. Spores or LC?


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Re: contam maybe in my wbs [Re: Doc_T]
    #16199564 - 05/08/12 12:00 PM (12 years, 8 months ago)

It was ms, thanks for the quick reply doc t, im going to throw them out. Appreciate it bro


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Re: contam maybe in my wbs [Re: kendawg]
    #16199590 - 05/08/12 12:07 PM (12 years, 8 months ago)

Double-check your sterilization procedures, especially times.
But I suspect you have a bad syringe. Drop a drop on an agar dish to see.
Also, you might be overfilling your jars. I like to use just two level cups of cooked grain in a quart jar.


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Re: contam maybe in my wbs [Re: Doc_T]
    #16199611 - 05/08/12 12:16 PM (12 years, 8 months ago)

I made 16 quarts with the same syringe all at the same time. Just these 2 looked funny. I did overload the jars, its a bitch to shake.


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Re: contam maybe in my wbs [Re: kendawg]
    #16199626 - 05/08/12 12:20 PM (12 years, 8 months ago)

The other jars might or might not be ok.
It's possible to have subtle slow contams that wait until after you spawn to make themselves known.
Use them with caution. Definitely break them up and wait for recolonization.

Save yourself the heartache next time.
Spores->agar->grain->GLC/g2g will get you all the spawn you can use in less time than you'd believe.
And yeah, overfilled jars don't always sterilize well. Bump your times up a bit or just use less.


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Re: contam maybe in my wbs [Re: Doc_T]
    #16199646 - 05/08/12 12:27 PM (12 years, 8 months ago)

Shit i just made 2 monos, the jars looked kick ass. I sure as hell hope nothing bad surprises me. Fuck i guess i will see in a few weeks. Thanks again for the advice and help bro :thumbup:


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