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Offlinenemesis19902008
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What is the best Karo LC/Water ratio?
    #16194022 - 05/07/12 09:36 AM (12 years, 8 months ago)

Need some advice my fellow mycologists.  The last LC i made with karo I used 2 teaspoons karo/250ml of water. I got pretty good results, but my LC never got more than 60% thick with myc.  I was wondering what everyone else uses as far as ratio of karo/water to get that nice thick myc filled LC?


--------------------
All of my photos are fabricated or retrieved from the internet and are not mine :smile:

This is my simple religion. There is no need for temples; no need for complicated philosophy. Our own brain, our own heart is our temple; the philosophy is kindness.

There's a part of every living thing that wants to become itself: the tadpole into the frog, the chrysalis into the butterfly, a damaged human being into a whole one. That is spirituality.

Edited by nemesis19902008 (05/07/12 10:04 AM)

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Re: What is the best Karo LC/Water ratio? [Re: nemesis19902008]
    #16194086 - 05/07/12 09:58 AM (12 years, 8 months ago)

Foggy LC is contaminated.


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Offlinenemesis19902008
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Re: What is the best Karo LC/Water ratio? [Re: Doc_T]
    #16194104 - 05/07/12 10:03 AM (12 years, 8 months ago)

Quote:

Doc_T said:
Foggy LC is contaminated.




Sorry for my lack of better words, I meant thick LC not foggy. My LC was not really thick, only about 60% nice myc growth. I even let it sit for about 2 weeks then inoculated some cakes with it.  100% success and no contams. I just want my LC to be thicker.


--------------------
All of my photos are fabricated or retrieved from the internet and are not mine :smile:

This is my simple religion. There is no need for temples; no need for complicated philosophy. Our own brain, our own heart is our temple; the philosophy is kindness.

There's a part of every living thing that wants to become itself: the tadpole into the frog, the chrysalis into the butterfly, a damaged human being into a whole one. That is spirituality.

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OfflineWing
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Re: What is the best Karo LC/Water ratio? [Re: Doc_T]
    #16194113 - 05/07/12 10:05 AM (12 years, 8 months ago)

Use Light Malt Extract if you're doing LC. Much better results and it adjusts to grains better than sugar based LC's.

GLC works even better than LC

And G2G even better than all the above.


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Re: What is the best Karo LC/Water ratio? [Re: nemesis19902008]
    #16194131 - 05/07/12 10:09 AM (12 years, 8 months ago)

Quote:

nemesis19902008 said:
I just want my LC to be thicker.




Why?

Edit- try malt.


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Offlinenemesis19902008
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Re: What is the best Karo LC/Water ratio? [Re: Doc_T]
    #16194137 - 05/07/12 10:11 AM (12 years, 8 months ago)

Quote:

Doc_T said:
Quote:

nemesis19902008 said:
I just want my LC to be thicker.




Why?




I guess its more of a visual thing really. Thicker myc/LC ratio seems like colonization time would be much quicker though.


--------------------
All of my photos are fabricated or retrieved from the internet and are not mine :smile:

This is my simple religion. There is no need for temples; no need for complicated philosophy. Our own brain, our own heart is our temple; the philosophy is kindness.

There's a part of every living thing that wants to become itself: the tadpole into the frog, the chrysalis into the butterfly, a damaged human being into a whole one. That is spirituality.

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Offlinenemesis19902008
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Re: What is the best Karo LC/Water ratio? [Re: Wing]
    #16194145 - 05/07/12 10:12 AM (12 years, 8 months ago)

Quote:

Wing said:
Use Light Malt Extract if you're doing LC. Much better results and it adjusts to grains better than sugar based LC's.

GLC works even better than LC

And G2G even better than all the above.




Thanks for the advice. I think I might try the malt extract LC method next time I make LC.


--------------------
All of my photos are fabricated or retrieved from the internet and are not mine :smile:

This is my simple religion. There is no need for temples; no need for complicated philosophy. Our own brain, our own heart is our temple; the philosophy is kindness.

There's a part of every living thing that wants to become itself: the tadpole into the frog, the chrysalis into the butterfly, a damaged human being into a whole one. That is spirituality.

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Offlinejoyless
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Re: What is the best Karo LC/Water ratio? [Re: nemesis19902008]
    #16194149 - 05/07/12 10:14 AM (12 years, 8 months ago)

Try GLC, its so much easier to use.

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Re: What is the best Karo LC/Water ratio? [Re: nemesis19902008]
    #16194150 - 05/07/12 10:14 AM (12 years, 8 months ago)

If you want faster colonization, forget LC.
You want to be working with agar, it's easier and cheaper than it looks.
Agar->grain->g2g will get you more spawn than you can use in less time than you'd believe.

But see my LC recipe linked above if you really want to do things the hard. way. :thumbup:


--------------------
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Offlinenemesis19902008
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Re: What is the best Karo LC/Water ratio? [Re: Doc_T]
    #16194165 - 05/07/12 10:18 AM (12 years, 8 months ago)

Quote:

Doc_T said:
If you want faster colonization, forget LC.
You want to be working with agar, it's easier and cheaper than it looks.
Agar->grain->g2g will get you more spawn than you can use in less time than you'd believe.

But see my LC recipe linked above if you really want to do things the hard. way. :thumbup:




Thank you for the information. I'm still new at this. Started with MS syringe with BRF cakes, then moved to karo LC with BRF cakes, and now I want to do LC with WBS and monotub with coir after the success.


--------------------
All of my photos are fabricated or retrieved from the internet and are not mine :smile:

This is my simple religion. There is no need for temples; no need for complicated philosophy. Our own brain, our own heart is our temple; the philosophy is kindness.

There's a part of every living thing that wants to become itself: the tadpole into the frog, the chrysalis into the butterfly, a damaged human being into a whole one. That is spirituality.

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InvisibleDoc_T
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Re: What is the best Karo LC/Water ratio? [Re: nemesis19902008]
    #16194174 - 05/07/12 10:19 AM (12 years, 8 months ago)

At best, LC is a side trip, a detour on the road to success.
It's fun sometimes, but not actually a productive endeavor.

Grain LC, mentioned above, is the best way to handle liquid inoculation.

But agar work really is cheaper and easier than it looks.
Newbs think they can't do it, but that's a myth.
College freshmen all over the country work with agar in Microbiology I. You can do it if they can.


--------------------
You make it all possible. Doesn't it feel good?

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Offlinenemesis19902008
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Re: What is the best Karo LC/Water ratio? [Re: Doc_T]
    #16194188 - 05/07/12 10:23 AM (12 years, 8 months ago)

Quote:

Doc_T said:
At best, LC is a side trip, a detour on the road to success.
It's fun sometimes, but not actually a productive endeavor.

Grain LC, mentioned above, is the best way to handle liquid inoculation.

But agar work really is cheaper and easier than it looks.
Newbs think they can't do it, but that's a myth.
College freshmen all over the country work with agar in Microbiology I. You can do it if they can.




I was actually reading Agar's grain LC tek the other day. It seemed like it was wayyyyy better, but you have to have a starting point. Which is why i figured making a master LC first would be a good idea. I have some popcorn cooking right now. Going to inoculate with the LC I am making and then attempt a grain LC from it once it is colonized.


--------------------
All of my photos are fabricated or retrieved from the internet and are not mine :smile:

This is my simple religion. There is no need for temples; no need for complicated philosophy. Our own brain, our own heart is our temple; the philosophy is kindness.

There's a part of every living thing that wants to become itself: the tadpole into the frog, the chrysalis into the butterfly, a damaged human being into a whole one. That is spirituality.

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InvisibleDoc_T
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Re: What is the best Karo LC/Water ratio? [Re: nemesis19902008]
    #16194206 - 05/07/12 10:29 AM (12 years, 8 months ago)

You have to have a starting point. But a syringe full of multispore genetics and potential contaminants isn't it.
Also, popcorn is the worst possible grain to use for mycology. Literally any other grain on earth is a better choice.

You've searched well but found old, outdated info.
A for effort, but your results are going to be C+ at best.
Always reorder your  search results by date, newest on top.


--------------------
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Offlinenemesis19902008
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Re: What is the best Karo LC/Water ratio? [Re: Doc_T]
    #16194222 - 05/07/12 10:33 AM (12 years, 8 months ago)

Quote:

Doc_T said:
You have to have a starting point. But a syringe full of multispore genetics and potential contaminants isn't it.
Also, popcorn is the worst possible grain to use for mycology. Literally any other grain on earth is a better choice.

You've searched well but found old, outdated info.
A for effort, but your results are going to be C+ at best.
Always reorder your  search results by date, newest on top.




Again I appreciate the information and advice.  I'm actually going to throw out the popcorn right now and find an alternative.


--------------------
All of my photos are fabricated or retrieved from the internet and are not mine :smile:

This is my simple religion. There is no need for temples; no need for complicated philosophy. Our own brain, our own heart is our temple; the philosophy is kindness.

There's a part of every living thing that wants to become itself: the tadpole into the frog, the chrysalis into the butterfly, a damaged human being into a whole one. That is spirituality.

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Re: What is the best Karo LC/Water ratio? [Re: nemesis19902008]
    #16194256 - 05/07/12 10:44 AM (12 years, 8 months ago)

That's the ratio I use. The jars and lids I use for my liquid cultures don't have filters for gas exchange so I will leave a fair amount of room for a pocket of air so the mycelium has some oxygen to use.

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Offlinebootsyyy
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Re: What is the best Karo LC/Water ratio? [Re: migraineur]
    #16194261 - 05/07/12 10:45 AM (12 years, 8 months ago)

8cc karo
192 cc water

thats what i use for a one pint jar

for a half pint i use half that.

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Offlinemigraineur
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Re: What is the best Karo LC/Water ratio? [Re: migraineur]
    #16194280 - 05/07/12 10:51 AM (12 years, 8 months ago)

Doc T, Do you use a laminar flow hood or a glove box?

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Re: What is the best Karo LC/Water ratio? [Re: migraineur]
    #16194316 - 05/07/12 11:02 AM (12 years, 8 months ago)

Quote:

migraineur said:
Doc T, Do you use a laminar flow hood or a glove box?




No, but you should. :thumbup:

A flowhood is more comfortable but doesn't give you any extra abilities vs a glove box.
You actually want a 'still air box', not a 'glove box' per se.

Quote:

nemesis19902008 said: I'm actually going to throw out the popcorn right now and find an alternative.




If it's already soaking you might as well prep it and use it.
But go get some rye grain for next time.


--------------------
You make it all possible. Doesn't it feel good?

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Offlinenemesis19902008
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Re: What is the best Karo LC/Water ratio? [Re: Doc_T]
    #16194420 - 05/07/12 11:34 AM (12 years, 8 months ago)

Quote:

Doc_T said:
Quote:

migraineur said:
Doc T, Do you use a laminar flow hood or a glove box?




No, but you should. :thumbup:

A flowhood is more comfortable but doesn't give you any extra abilities vs a glove box.
You actually want a 'still air box', not a 'glove box' per se.

Quote:

nemesis19902008 said: I'm actually going to throw out the popcorn right now and find an alternative.




If it's already soaking you might as well prep it and use it.
But go get some rye grain for next time.




Okay thanks for the advice. I'm going to try and find some rye grain. If not then I know that I can find WBS.


--------------------
All of my photos are fabricated or retrieved from the internet and are not mine :smile:

This is my simple religion. There is no need for temples; no need for complicated philosophy. Our own brain, our own heart is our temple; the philosophy is kindness.

There's a part of every living thing that wants to become itself: the tadpole into the frog, the chrysalis into the butterfly, a damaged human being into a whole one. That is spirituality.

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Re: What is the best Karo LC/Water ratio? [Re: nemesis19902008]
    #16194423 - 05/07/12 11:35 AM (12 years, 8 months ago)

You really don't have to quote every post in the thread. Just click 'reply' most of the time.


--------------------
You make it all possible. Doesn't it feel good?

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Offlinenemesis19902008
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Re: What is the best Karo LC/Water ratio? [Re: Doc_T]
    #16194434 - 05/07/12 11:37 AM (12 years, 8 months ago)

Right lol I just noticed that it was doing that. Still new to message boards as well.


--------------------
All of my photos are fabricated or retrieved from the internet and are not mine :smile:

This is my simple religion. There is no need for temples; no need for complicated philosophy. Our own brain, our own heart is our temple; the philosophy is kindness.

There's a part of every living thing that wants to become itself: the tadpole into the frog, the chrysalis into the butterfly, a damaged human being into a whole one. That is spirituality.

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Re: What is the best Karo LC/Water ratio? [Re: nemesis19902008]
    #16194735 - 05/07/12 12:53 PM (12 years, 8 months ago)

If you can't find rye then you can use wheat and other grains.

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Re: What is the best Karo LC/Water ratio? [Re: migraineur]
    #16194741 - 05/07/12 12:54 PM (12 years, 8 months ago)

Doc T, could you please show me a photo of your glove box or still air box?

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Re: What is the best Karo LC/Water ratio? [Re: migraineur]
    #16194764 - 05/07/12 12:59 PM (12 years, 8 months ago)

No, because I don't use one. 
But I can link you to Roger Rabbit's videos, do it like that: http://www.mushroomvideos.com/


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You make it all possible. Doesn't it feel good?

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Re: What is the best Karo LC/Water ratio? [Re: Doc_T]
    #16195286 - 05/07/12 03:04 PM (12 years, 8 months ago)

What do you use if you don't use a flow hood or glove box etc?

I own a copy of his DVD already. I haven't watched it for a while though.

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Re: What is the best Karo LC/Water ratio? [Re: migraineur]
    #16195364 - 05/07/12 03:18 PM (12 years, 8 months ago)

I use excellent sterile technique. But you need to use a still air box or a flowhood.
I have had years of experience, and prior to that I enjoyed the luxury of professional training.

A SAB is simpler to use than a flowhood in some ways, you don't have to be as rigorous with hand motion, position, etc.
A flowhood is a tool which requires correct use, same as any tool. SAB isn't as picky (if the air is still).

I've told you how I think you should do it.
Do it like that, or don't. Your choice.


--------------------
You make it all possible. Doesn't it feel good?

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