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joyless
Stranger
Registered: 05/18/10
Posts: 97
Last seen: 1 year, 10 months
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Quote:
spornication said: you can reuse your plastic petri dishes with a sanitary dillution of bleach should never buy pre poured dishes your just asking for contams
thats BS. where i buy my pre poured plates, they come vaacum sealed with lab quality check and bacterial count.
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MagicCarpetRide89
Sideways Traveler



Registered: 12/24/11
Posts: 1,126
Loc: usa
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Re: Agar Procedure Help!! [Re: Doc_T]
#16200882 - 05/08/12 05:49 PM (12 years, 8 months ago) |
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Should I clone from my forth flush? Or would that not work so good?
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Edited by MagicCarpetRide89 (05/08/12 07:23 PM)
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MagicCarpetRide89
Sideways Traveler



Registered: 12/24/11
Posts: 1,126
Loc: usa
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....bump.....
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bishlap
Po Thead



Registered: 01/04/12
Posts: 4,085
Last seen: 6 years, 1 month
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it should be fine but I would isolate from it asap then inoc a few more plates and get your isolate if you didn't already.
time I assume would be your enemy because of senescence, were they from g2g?
-------------------- "If you're not worried that you took way
to much, you didn't take enough" -
Terrence McKenna
There is no soul, only the ego dies.
The body was never yours.
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MagicCarpetRide89
Sideways Traveler



Registered: 12/24/11
Posts: 1,126
Loc: usa
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Re: Agar Procedure Help!! [Re: bishlap]
#16206202 - 05/09/12 06:21 PM (12 years, 8 months ago) |
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No, MS. But the first 3 flushes were real nice. I wish I cloned from one of those first three. You think even if I clone from this last flush, I will still be isolating a good clone?
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bishlap
Po Thead



Registered: 01/04/12
Posts: 4,085
Last seen: 6 years, 1 month
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the sample will likely have many "strains" in it. I'm assuming you want to save your isolates for later use in slants so if that's the case you'll want to try to get your isolates taken as soon as you see sectoring to multiple other dishes from each healthy sector
grow those till/if they sector and isolate again if needed, place samples in slants, grow out each isolate and decide what one you like best then use that slant to colonize more agar > grain > g2g > whatever floats your boat
remember to label everything, or life will brcome difficult for you later.
the only issue I can think of from cloning soo late is senescence weaking your culture but you should be fine if your diligent.
-------------------- "If you're not worried that you took way
to much, you didn't take enough" -
Terrence McKenna
There is no soul, only the ego dies.
The body was never yours.
Edited by bishlap (05/09/12 07:05 PM)
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