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OfflineShredHead
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Registered: 04/12/12
Posts: 8
Last seen: 12 years, 6 months
inoculation 2 Weeks Past, No Results.
    #16185936 - 05/05/12 12:28 PM (12 years, 8 months ago)

Hey guys

I purchased some spores from the shop I work at and inoculated them using RR's BRF Tek about 2 weeks ago. I made 5 jars just to test it out, and planned on innoculating the rest after I noticed some growth on these ones. Its been nearly 2 weeks, and I have had no growth. No contams appear to be growing, but im not seeing any good white mycellium growth either. I steam sterilized for 90 mins, and used Lysol to clean my work area. I used Isoproply to clean my syringe, and used a lighter until it was red hot before innoculating. I cleaned the needle after each jar as well.

-I guess my concern is if there is a possibility that I wont see any growth for this long.. and is that normal?

-Can the spores die after sitting too long? My boss mentioned that they replace them every 4 months or something, but I have never seen it happen. Can the spores become innactive and just plain fail? No growth of contams, and no healthy growth.

-Is it worth cleaning the jars out, and snagging another syringe from my shop? And is it a possibility I messed something up along the way? I used the recipie in RR's Videos as well for the 1/2 pint BRF cakes. I just kinda wonder if I bought a dud, there are 4 other ones available.

- They also werent labled, and all anyone can tell me about them is they are "Gold Caps", which from what I've been reading here generally just means they are Cubes. but the strain is unknown to the people selling me them haha. They are black and wispy inside the syringe, probably not gonna help with IDing, but worth a shot.


I appreciate any and all responses, so thank you in advance!

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OfflineSimpleFarmer
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Registered: 04/04/12
Posts: 3,973
Loc: gumby land
Last seen: 6 years, 10 months
Re: inoculation 2 Weeks Past, No Results. [Re: ShredHead]
    #16185952 - 05/05/12 12:34 PM (12 years, 8 months ago)

They could be bunk (hope not) . Did you press against the glass when you inoculated? I had the same problem but I didn't press against the glass and at about 2 weeks they started coming threw .. just wait a week and if nothing by then scrap and start over ..  :2cents:

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Offlineinfinitesidedcube
Stardust Disintegrator
Male


Registered: 09/05/11
Posts: 604
Loc: Hourglass Nebula
Last seen: 11 years, 11 months
Re: inoculation 2 Weeks Past, No Results. [Re: SimpleFarmer]
    #16185972 - 05/05/12 12:40 PM (12 years, 8 months ago)

Quote:

SimpleFarmer said:
They could be bunk (hope not) . Did you press against the glass when you inoculated? I had the same problem but I didn't press against the glass and at about 2 weeks they started coming threw .. just wait a week and if nothing by then scrap and start over ..  :2cents:



:whathesaid:


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:mushroom2:Shpongle - Shpongle Spores (HD):mushroom2:

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OfflineShredHead
Stranger
Registered: 04/12/12
Posts: 8
Last seen: 12 years, 6 months
Re: inoculation 2 Weeks Past, No Results. [Re: infinitesidedcube]
    #16187002 - 05/05/12 05:30 PM (12 years, 8 months ago)

Thanks for the fast response guys

Yeah i watched the liquid flow down the side of the jars. So based on other topics ive been reading, I finished off the rest of my Innoculant in the 5 jars, and since the spores were blackish, NOW the areas where i inoculated are tinted with black from the new dosage of spores, where as before there was no color, the liquid went on clear. Have your spores left your substrate colored in the past? Or generally its just pretty clear?

Thank you again.

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InvisibleDoc_T
Random Dude
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Re: inoculation 2 Weeks Past, No Results. [Re: ShredHead]
    #16187020 - 05/05/12 05:33 PM (12 years, 8 months ago)

You re-inoculated the same jars? And used up all your spores?
Bummer man. Get more spores. Better luck next time!


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OfflineSimpleFarmer
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Registered: 04/04/12
Posts: 3,973
Loc: gumby land
Last seen: 6 years, 10 months
Re: inoculation 2 Weeks Past, No Results. [Re: ShredHead]
    #16187022 - 05/05/12 05:34 PM (12 years, 8 months ago)

same jar as of now

Edited by SimpleFarmer (05/05/12 05:38 PM)

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InvisibleDoc_T
Random Dude
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Folding@home Statistics
Registered: 03/06/09
Posts: 42,395
Loc: Colorado Flag
Re: inoculation 2 Weeks Past, No Results. [Re: SimpleFarmer]
    #16187062 - 05/05/12 05:42 PM (12 years, 8 months ago)

Is that a sunflower seed?


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You make it all possible. Doesn't it feel good?

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OfflineSimpleFarmer
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Registered: 04/04/12
Posts: 3,973
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Re: inoculation 2 Weeks Past, No Results. [Re: Doc_T]
    #16187069 - 05/05/12 05:44 PM (12 years, 8 months ago)

Lol big clump of spores ... I forgot to shake :frown: :shrug:

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Offlinek00laid
NEMO
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Registered: 05/03/10
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Re: inoculation 2 Weeks Past, No Results. [Re: SimpleFarmer]
    #16187243 - 05/05/12 06:18 PM (12 years, 8 months ago)

:jawdrop:

looks gooey


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OfflineSimpleFarmer
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Registered: 04/04/12
Posts: 3,973
Loc: gumby land
Last seen: 6 years, 10 months
Re: inoculation 2 Weeks Past, No Results. [Re: k00laid]
    #16187266 - 05/05/12 06:21 PM (12 years, 8 months ago)

The clump?

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Offlinegobblegobble
dimitri says hi
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Registered: 03/13/12
Posts: 90
Last seen: 11 years, 9 months
Re: inoculation 2 Weeks Past, No Results. [Re: SimpleFarmer]
    #16187379 - 05/05/12 06:45 PM (12 years, 8 months ago)

Yo I did like 16 jars, none colonized at all after 1-2 weeks...I smelled inside them and it reeked bad so I figured they were bad, but nothing was growing in them, at least that I could see. Any suggestions on my problem? I already threw them out because they smelled bad :S


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Drugs are bad, except the good ones

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