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OfflineDeej3987
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Mycilium on Agar?
    #16167713 - 05/01/12 08:01 PM (12 years, 8 months ago)

Just want to make sure this is mycilium. I inoculated with ms syringe.

Black Poplar (I know the white spot in the top right is a contam)


Lions Mane (has not grown any for 2 weeks)


Both on a bigger petri dish (I'm pretty sure lions Mane on the left and black poplar on the right {they connected})



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Edited by Deej3987 (05/02/12 10:33 AM)

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OfflineDrCrumbs
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Re: Mycilium on Agar? [Re: Deej3987]
    #16167815 - 05/01/12 08:21 PM (12 years, 8 months ago)

This was from spore or LC?

Looks like it to me.

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OfflineDeej3987
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Re: Mycilium on Agar? [Re: DrCrumbs]
    #16168807 - 05/01/12 11:17 PM (12 years, 8 months ago)

Sorry should have mentioned this is from spores


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Re: Mycilium on Agar? [Re: Deej3987]
    #16169046 - 05/02/12 12:16 AM (12 years, 8 months ago)

Mycelium is generally fluffy and white.  The top pic (Black Poplar) looks like mycelium from the pic.  The other ones look shiny and smooth (glabrous), which would indicate that it's some sort of contaminant.  Looking under a microscope could help you out, but the smell should tell you a lot.  If it smells mushroomy, you're probably alright.  If it stinks, you're sunk.

Edited by shroomtomb (05/02/12 12:31 AM)

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OfflineLord_McLovin
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Re: Mycilium on Agar? [Re: Deej3987]
    #16169503 - 05/02/12 03:12 AM (12 years, 8 months ago)

You might want to have this thread moved to the edible forums. They are more likely to know how the mycelium of your specific species is supposed to look like.

Did you use a spore syringe for inoculating these? You should make sure to only use one drop or 2. I, personally, prefer going over the print with a swab and then using it to inoculate agar.


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InvisibleDoc_T
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Re: Mycilium on Agar? [Re: Lord_McLovin]
    #16169857 - 05/02/12 07:08 AM (12 years, 8 months ago)

Looks bacterial to me. :shrug:


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Offlinealex591955
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Re: Mycilium on Agar? [Re: Doc_T]
    #16170204 - 05/02/12 09:24 AM (12 years, 8 months ago)

If you think about it, using spores would put lots of different staring points on the agar. When i was messing around i could see a line of where all the staring points from where the liquid ran to with the spores. the Black Poplar first pic is definitely mycelium growing and you can see that as a example of the water running in that picture as well as in the last pic, should also be easy to transfer over to another dish and move away from that contam. The next one down of the lion’s mane is a contam. The last one looks like puffy myc to me. See how you can tell where the liquid ran to after it was put on the agar and all the different start points? Also you can see on the contam dishes that the contam started in a single spot and grew out evenly. Are you sure you put some spores on the lion’s mane? Sometimes I’ll miss one in my GB and later find out nothing grew. Also sense there is obvious growth on the other dishes, I’m surprised not to see any on the lion’s mane dish.

On that last dish though, you can see the difference in the strains in the mycelium. Could get lucky and cross a strain :smile: you should be able to see sectors on the agar. Each sector is a strain. If you had two strains merge into one when they come into contact then it would be a cross of the two. But the likeliness of that is something like 100 to 1 if you’re lucky:)


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OfflineDeej3987
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Re: Mycilium on Agar? [Re: alex591955]
    #16170470 - 05/02/12 10:44 AM (12 years, 8 months ago)

I was worried the bottom 2 were contamed, but the second pic of lions mane mycilium looks similar to the mycilium on the left of the bottom pic. I will check with the other forum also since I am having trouble with these strains to see if they recognize the growth. Thanks guys for your advice.


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OfflineDrCrumbs
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Re: Mycilium on Agar? [Re: Deej3987]
    #16170491 - 05/02/12 10:49 AM (12 years, 8 months ago)

Keeps us updated, like to see some more pics in a few days.

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OfflineDeej3987
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Re: Mycilium on Agar? [Re: DrCrumbs]
    #16185927 - 05/05/12 12:26 PM (12 years, 8 months ago)

I was told that these are all contamed from the other forum..


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Offlinefungusapien
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Re: Mycilium on Agar? [Re: Deej3987]
    #16185973 - 05/05/12 12:40 PM (12 years, 8 months ago)

Yeah, I'd say they're correct.  Most of that looks like bacteria, with the exception of the first photo, which might be fungal, but doesn't quite look like Hericium.


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