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Offlinetibberous
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Registered: 12/26/04
Posts: 1,194
Last seen: 1 year, 3 months
Oil Layer to help prevent contams when sucking up LC's?
    #16184920 - 05/05/12 05:47 AM (12 years, 8 months ago)

Hey guys, think I might of come up with a good idea.

Add something like oil or paraffin to the LC - it should float on top of the water.

Then, when you suck up the LC, any bacteria / spores that might blow in would land on the protective layer, but you'd suck the LC up from the bottom.

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Offlinehenik0
one of those, cool guys.


Registered: 11/18/10
Posts: 282
Loc: Great Lakes maybe
Last seen: 8 years, 5 months
Re: Oil Layer to help prevent contams when sucking up LC's? [Re: tibberous]
    #16184963 - 05/05/12 06:29 AM (12 years, 8 months ago)

It would prevent too much gas exchange between the LC and the air because of oils low vapor pressure. Just create a proper LC jar. Interesting idea though.


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OfflineRogerRabbitM
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Registered: 03/26/03
Posts: 42,214
Loc: Seattle
Last seen: 1 year, 10 months
Trusted Cultivator
OG Cultivator
Re: Oil Layer to help prevent contams when sucking up LC's? [Re: henik0]
    #16185000 - 05/05/12 06:50 AM (12 years, 8 months ago)

That's not where contaminants enter a liquid culture.  They enter when you do an inoculation with spores, which by nature are not sterile and often contain other mold spores and bacteria.  This is why I recommend against LC.
RR


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Offlinetibberous
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Registered: 12/26/04
Posts: 1,194
Last seen: 1 year, 3 months
Re: Oil Layer to help prevent contams when sucking up LC's? [Re: RogerRabbit]
    #16185720 - 05/05/12 11:29 AM (12 years, 8 months ago)

Quote:

RogerRabbit said:
That's not where contaminants enter a liquid culture.  They enter when you do an inoculation with spores, which by nature are not sterile and often contain other mold spores and bacteria.  This is why I recommend against LC.
RR




One thing I didn't realize until recently was how much spore-quality mattered when doing LC's. SporeWorks is like 50/50, but Ralphsters fail nearly 100% of the time. (lil shop is in between, but way better than Ralphsters still)

%50 sounds bad, but if you do 24 jars, get 12, and take 2, 30cc syringes, that gives you a ton of LC. There just so easy to do and so fast to colonize, it's almost worth the waste / mess / contams. Figure a rye bag is like $.90, potato LC is is like $.03 -- doesn't matter if you loose a few.

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