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InvisibleLucidDream
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Stalled cakes, even after flipping
    #1618398 - 06/08/03 03:25 PM (13 years, 6 months ago)

PF jars in an incubator at 80 degrees and high humidity. Jars have metal lids in place with holes, and a loose foil covering. Jars were flipped once outer colonization was between 50-70%. (Foil was left in place due to excess moisture in the incubator.) This helped spur some new growth for a day or two, then it stalled. So the lids were loosened to get some more air in there. Nothing. These cakes are stalled at 3-1/2 weeks with the best one at about 70% colonization. They don't seem to want to budge. A couple of the cakes are a tad on the sweaty side, but no pooled water. Nice healthy white fuzz.

On the verge of taking the lids off and just using the foil if something doesn't happen soon. Tempted to take a sterile syringe and inject a couple cc's of air in the bastards, though it seems like a foolish idea. How can the babies get some safe air? No flow hoods in this po' boy's future.

I can almost hear the voices counseling, "Patience, Grasshopper..."


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Invisiblemicro
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Re: Stalled cakes, even after flipping [Re: LucidDream]
    #1618401 - 06/08/03 03:28 PM (13 years, 6 months ago)

Where in the jars did it stall (bottom, top, middle?) Is the sweet smell you describe somewhat like wine or garbage?

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InvisibleKlingonFromUranus
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Re: Stalled cakes, even after flipping [Re: micro]
    #1618422 - 06/08/03 03:42 PM (13 years, 6 months ago)

Is the stalled area a different color, i.e. darker etc? Do the jars emit a funky smell? I had one jar stall recently and it turneds out to be a bacterial contam. The uncolonized cake looked the same as it always did, but it smelled like gym socks.


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InvisibleLucidDream
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Re: Stalled cakes, even after flipping [Re: KlingonFromUranus]
    #1618427 - 06/08/03 03:45 PM (13 years, 6 months ago)

There is no smell. I said they were somewhat sweaty, but not overly so. The bottom of the jars (which is now the top) is where the colonization has stalled. They BRF there looks just the same as it ever did. No gym socks yet.


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Invisiblemicro
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Re: Stalled cakes, even after flipping [Re: LucidDream]
    #1618438 - 06/08/03 03:54 PM (13 years, 6 months ago)

Are you using a heating-pad, or space-heater?

Bottom sounds like an air exchange problem, but it could possibly be too much water in the substrate or heat damage.

If you flipped them over and they made a ('sssssssccchhhhhhhhhh') noise it was prolly CO2, so just wait longer. If the sweet smell turns into a wine smell you have yeast infection; this could have been introduced when flipping the jars, so be careful.

I would say wait like another week and if there is no progress there is a problem. Just make sure if you are using a heating pad the glass bulb part of the thermometer is acually resting directly on the source of heat. If you flipped them, air should not be a problem from then on unless you have tape over the holes.

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InvisibleLucidDream
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Re: Stalled cakes, even after flipping [Re: micro]
    #1618460 - 06/08/03 04:05 PM (13 years, 6 months ago)

The incubator is a Rubbermaid with a fish-tank heater submerged in a quart Ball jar with a touch of peroxide mixed in with the water. The thermometer is suction-cupped to the side of the rubbermaid, measuring the air temp, which never exceeds 80 degrees. The lid of the rubbermaid is slightly askew to let excess moisture escape before it pools on the bottom.


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OfflineFunkyBudah
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Re: Stalled cakes, even after flipping [Re: LucidDream]
    #1619022 - 06/08/03 09:04 PM (13 years, 6 months ago)

Try raising the temps a little to 84-88 F. If it never goes above 80, it may be a little cool for optimum growth.

-FB


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Offlinephotovorix
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Re: Stalled cakes, even after flipping [Re: FunkyBudah]
    #1619148 - 06/08/03 10:01 PM (13 years, 6 months ago)

The bottom of the squirrels jars took a while to colonize too... They did eventualy though. The inside is likely colonizing right now, thus you dont see any outer colonization. The squirrel cut one he thought was stalled (at the bottom of the jar) in half, and found that there was a funnel shaped uncolonized area leading up to the small patch of (precut) visible uncolonized area.

On a side note, I bought a saw today. Just a BLACK&PECKER 7 1/4 inch circular saw, going to get a skill when i get my check


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Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation

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