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OfflineThe Influence
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Re: Reinoculate if jars do not colonize? [Re: Mr. Wilson] * 1
    #16184161 - 05/04/12 11:25 PM (12 years, 8 months ago)

Quote:

Mr. Wilson said:
im curious too why its been two weeks and no action--ive heard of slow spores -just not seen em yet--he should have seen something by now--slow spores+no GE=double whammy??--im still kind of a noob too---id like to see the guy get something for his effort--whether boomers or a learning experience



Yeah I'm scratching my head as well. I am no expert but have been at this awhile now and never had this happen. Personally though I would just throw them out, maybe take the tape off and wait a few more days but to me it's already been to long lol, I just freaked cause the jars I got colonizing now took 6 days to show signs of growth. I have gotten used to seeing growth in my jars from an ms inoculation in 3-4 days. It sounds like some of his jars are going so it's not a total loss.

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Invisiblecubes4cancer
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Re: Reinoculate if jars do not colonize? [Re: The Influence]
    #16184204 - 05/04/12 11:35 PM (12 years, 8 months ago)

i think op innoced the dry verm layer in these jars,if you didnt watch each innoc point to see if the spore water ran down the side of the glass,its probably the case.its happened to me before,i just reinnoced soon after i reckonized my problem. :thumbup:


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$$"IM ON A RAMAN NOODLE EVERY NIGHT BUDGET"$$
                                        :ramen:



ROGERRABBIT: P cubensis will grow on stuffed animals, bibles, quarter pounds of weed, bras, etc.
In other words,it's hard to fuck up a cube grow.
RR

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OfflineThe Influence
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Re: Reinoculate if jars do not colonize? [Re: cubes4cancer]
    #16184504 - 05/05/12 12:59 AM (12 years, 8 months ago)

Quote:

cubes4cancer said:
i think op innoced the dry verm layer in these jars,if you didnt watch each innoc point to see if the spore water ran down the side of the glass,its probably the case.its happened to me before,i just reinnoced soon after i reckonized my problem. :thumbup:



Hhhm maybe he did, but in that case that is even riskier business to re-inoculate then. Your dry verm layer is well supposed to be dry so that any contaminate spores can not germinate and colonize down to the substrate. If this is the case I would definitely say throw them out and that you sir got very lucky.

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OfflineMr. Wilson
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Re: Reinoculate if jars do not colonize? [Re: NeuronicObserver]
    #16185094 - 05/05/12 07:31 AM (12 years, 8 months ago)

well neuronicO what have you done with em?? inquiring minds want to know--


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Offlinebulsie
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Re: Reinoculate if jars do not colonize? [Re: Mr. Wilson]
    #16185133 - 05/05/12 07:46 AM (12 years, 8 months ago)

i had the same problem awhile back 99% of mine was lack of air exchange took regular tape off and put on micro pore and everything started mycing up

also mabey lack of correct temp?

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OfflineMr. Wilson
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Re: Reinoculate if jars do not colonize? [Re: The Influence]
    #16185158 - 05/05/12 07:56 AM (12 years, 8 months ago)

how would you solve the temp issue if that was it??  aargh--meant for bulsie


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Edited by Mr. Wilson (05/05/12 07:57 AM)

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OfflineRogerRabbitM
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Re: Reinoculate if jars do not colonize? [Re: Mr. Wilson]
    #16185284 - 05/05/12 09:08 AM (12 years, 8 months ago)

Quote:

Mr. Wilson said:
micro dont breathe as good as some think it does---that verm layer is really sufficient in itself---but anykind of tape or anything is gonna hinder gas exchange--even the coffee filter to some extent




Nonsense.

Gasses will easily penetrate micropore tape.  Masking tape is a different story.  Coffee filters in mycology are dumb-They're like trying to catch mosquitoes with a butterfly net.

To the OP, two points to consider:
1.  Did you allow steam to vent for several minutes before sealing up the pressure cooker to allow it to build pressure?  If you failed to do this, you had 15 psi of air pressure and it won't do squat.  You need 15 psi of steam pressure to sterilize and the only way to get this is to allow all the air to vent out first before sealing the unit.

2. Never re-inoculate.  If the jars didn't colonize, there's a reason.  Make up new jars and try again.
RR


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OfflineMr. Wilson
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Re: Reinoculate if jars do not colonize? [Re: RogerRabbit]
    #16185369 - 05/05/12 09:47 AM (12 years, 8 months ago)

well there you go OP--thats the final word--RR-i use the coffee filters to catch dem bastard fungus gnats--and it just makes me feel better--and yeah you gotta let that steam start coming out the tube good and heavy before you put the jiggler on--thats in the instructions of your PC OP--wish ya the best of success--and i would solve a colonizing temp issue by just leaving em on a shelf at room temp--i still believe it was the masking tape that gotcha--good luck


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Edited by Mr. Wilson (05/05/12 09:54 AM)

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OfflineNeuronicObserver
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Re: Reinoculate if jars do not colonize? [Re: RogerRabbit]
    #16185393 - 05/05/12 09:54 AM (12 years, 8 months ago)

Temperature is right at 80F

I'll be getting some micropore tape today.

I am reasonably sure that the inoculation went past the dry verm layer, I'll watch that more closely with this next PC batch.

Using this PC: http://www.gopresto.com/products/products.php?stock=01282

@RR, the design of this PC seems to manage the steam build up automatically. I followed the directions for it, there was no indication of needing to allow steam buildup prior to sealing.

Notes:
A few of the jars that are having trouble were compacted from PC, so there is a gap between the dry verm layer and lid. This was made worse by how the dry verm is fine grade, it fell down the sides of the jars to some extent. This however doesn't solve the problem because there are some new jars which have a much smaller gap and no exterior verm fallout.

With my third PC, I'll make sure the jars are extra compact(within reason), to eliminate the gap 100%. I'm not sure whether the verm will fall down again if I use fine, so maybe I should stick with coarse for the verm layer?

Thanks guys for all of the advice, I feel welcomed :smile:


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OfflineNeuronicObserver
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Re: Reinoculate if jars do not colonize? [Re: Mr. Wilson]
    #16185408 - 05/05/12 09:58 AM (12 years, 8 months ago)

Quote:

Mr. Wilson said:
well there you go OP--thats the final word--RR-i use the coffee filters to catch dem bastard fungus gnats--and it just makes me feel better--and yeah you gotta let that steam start coming out the tube good and heavy before you put the jiggler on--thats in the instructions of your PC OP--wish ya the best of success--and i would solve a colonizing temp issue by just leaving em on a shelf at room temp--i still believe it was the masking tape that gotcha--good luck




I'm looking at the manual right now. It says to seal the pressure cooker and put on the pressure regulator(jiggler) prior to heating.


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OfflineMr. Wilson
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Re: Reinoculate if jars do not colonize? [Re: NeuronicObserver]
    #16185437 - 05/05/12 10:09 AM (12 years, 8 months ago)

hmmm--i just wait till that steam starts comin out and grab the jiggler with a dish towel and put it on--wait till you hit 15 lbs-reduce the heat a little--like from high to bout med high--start timer-i run for 75 minutes--i have NO problems


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OfflineNeuronicObserver
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Re: Reinoculate if jars do not colonize? [Re: Mr. Wilson]
    #16185471 - 05/05/12 10:21 AM (12 years, 8 months ago)

Quote:

Mr. Wilson said:
hmmm--i just wait till that steam starts comin out and grab the jiggler with a dish towel and put it on--wait till you hit 15 lbs-reduce the heat a little--like from high to bout med high--start timer-i run for 75 minutes--i have NO problems




I'll try it :smile:


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OfflineMr. Wilson
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Re: Reinoculate if jars do not colonize? [Re: NeuronicObserver]
    #16185491 - 05/05/12 10:25 AM (12 years, 8 months ago)

got time--wanna start another run--ill babysit ya and make sure you get it right--im sitting here thinking bout starting a run myself


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OfflineNeuronicObserver
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Re: Reinoculate if jars do not colonize? [Re: Mr. Wilson]
    #16185686 - 05/05/12 11:20 AM (12 years, 8 months ago)

Quote:

Mr. Wilson said:
got time--wanna start another run--ill babysit ya and make sure you get it right--im sitting here thinking bout starting a run myself




I just have to fight through this final exam for college, then I'll be free to mycologize! I'm hoping to PC at around 6 or 7 today. Btw your jars are looking beautiful.


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OfflineSimpleFarmer
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Re: Reinoculate if jars do not colonize? [Re: Mr. Wilson]
    #16185694 - 05/05/12 11:23 AM (12 years, 8 months ago)

Pure fucking nonsense  !!! Lol

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OfflineMr. Wilson
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Re: Reinoculate if jars do not colonize? [Re: SimpleFarmer]
    #16186047 - 05/05/12 01:03 PM (12 years, 8 months ago)

whaaa???


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OfflineSimpleFarmer
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Re: Reinoculate if jars do not colonize? [Re: Mr. Wilson]
    #16186060 - 05/05/12 01:06 PM (12 years, 8 months ago)

Lol just giving ya shit

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OfflineMr. Wilson
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Re: Reinoculate if jars do not colonize? [Re: SimpleFarmer]
    #16186094 - 05/05/12 01:13 PM (12 years, 8 months ago)

tell me what you really think about me directing this dude-please dont be politr--im thick skinned-i can deal--i just wanna help as much as yall helped me--if im an idiot and just got lucky this time just tell me--lol


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OfflineRogerRabbitM
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Re: Reinoculate if jars do not colonize? [Re: Mr. Wilson]
    #16186104 - 05/05/12 01:16 PM (12 years, 8 months ago)

Quote:

Mr. Wilson said:
hmmm--i just wait till that steam starts comin out and grab the jiggler with a dish towel and put it on--wait till you hit 15 lbs-reduce the heat a little--like from high to bout med high--start timer-i run for 75 minutes--i have NO problems




This is the correct method, even if the directions for the pressure cooker say to put the weight on first.
RR


--------------------
Download Let's Grow Mushrooms



semper in excretia sumus solim profundum variat

"I've never had a failed experiment.  I've only discovered 10,000 methods which do not work."
Thomas Edison

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OfflineSimpleFarmer
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Re: Reinoculate if jars do not colonize? [Re: Mr. Wilson]
    #16186122 - 05/05/12 01:21 PM (12 years, 8 months ago)

Your doing fine man , I just like giving advice on things I know and can show them a trusted cultivator  said so too .. I know people bitch about people that don't use the search bar but I like it bc I can go and search for what they are asking for and hook them up with the link and in the process learn it myself :wink: a lot of people call it spoon feeding but I call it helpful research ..you are doing good man keep up the good work

Edited by SimpleFarmer (05/05/12 01:25 PM)

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