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OfflineALTERED.STATES
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Rye grain jar syringe inoculation question
    #16179929 - 05/04/12 12:40 AM (12 years, 8 months ago)

How do you inoculate directly into a rye grain jar using a syringe?

If the jar is 2/3 full to allow for shaking does this mean the needle usually doesn't make contact with the grain and the syringe contents are ejected aiming towards the sides if possible and the contents allowed to run down the inside of the glass down, into and through the rye?


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InvisibleBeefy1
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Re: Rye grain jar syringe inoculation question [Re: ALTERED.STATES]
    #16179940 - 05/04/12 12:43 AM (12 years, 8 months ago)

I aim for the side so it drips down and hopefully gets the bottom started at the same time as the top.


don't shake until you've got growth,  you want the spores close to eachother when they sprout.

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Re: Rye grain jar syringe inoculation question [Re: Beefy1]
    #16180072 - 05/04/12 01:33 AM (12 years, 8 months ago)

I'm pretty sure Beefy answered it but if your looking more for a tek on a way to keep everything sterile all I do is make self healing inoculation ports out of rtv silicone, flame sterilize, wipe needle with an alcohol patch and then quickly put the needle in and inject the spores. I do 1 cc per pint and it works fine for me but I'm frugal when it comes to spores.


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OfflineALTERED.STATES
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Re: Rye grain jar syringe inoculation question [Re: mark6587]
    #16180645 - 05/04/12 07:28 AM (12 years, 8 months ago)

ok sounds like what I thought. Thanks.


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Level 5+ 7g of Thai in NRG tek Trip Report and Pics here. Real spirit contact. :crazy2: Amazonian PES here. 2nd here 3rd here. :sun: Thai here 2nd here 3rd here :mushroom2: B+ here :tongue2: McKennaii here :ooo: ENORMOUS 26g Shroom here :shocked: 5g! Coin sized!! (PPS). 00 Gel Caps :rocket:


NRG Tek Drink (a.k.a Monster Tek Drink) here. Super lemon tek. Best experience. :crazy2:

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OfflineDazed Belief
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Re: Rye grain jar syringe inoculation question [Re: Beefy1]
    #16180671 - 05/04/12 07:40 AM (12 years, 8 months ago)

Quote:

Beefy1 said:
I aim for the side so it drips down and hopefully gets the bottom started at the same time as the top.


don't shake until you've got growth,  you want the spores close to eachother when they sprout.




yup dont want to shake want to keep the spores together so they can form a dikaryon


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Re: Rye grain jar syringe inoculation question [Re: Dazed Belief]
    #16180855 - 05/04/12 09:18 AM (12 years, 8 months ago)

Yup, what they all said.
Furthermore, you want to make sure to make a circular motion w/ the syringe while inoculating.
You want the spores to hit 360 degrees of the interior glass.
Here's a picture to help show what I just described (the jars on the right are the best example):


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OfflineALTERED.STATES
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Re: Rye grain jar syringe inoculation question [Re: TranscendingLife]
    #16180866 - 05/04/12 09:23 AM (12 years, 8 months ago)

Yeah the swirl is pretty pronounced on the right. Thanks.

I have some Ecuadorian and Golden teacher I got in vials.

So I am looking to try inoculating directly to a grain jar this time.


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