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OfflineBlake_Shroom
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what the fuck is happening
    #16176722 - 05/03/12 01:46 PM (12 years, 8 months ago)

Every fucking jar i do gets fucking trich. i got trich in a tub a few weeks ago, brought it outside and disposed of it. now i know that i used a few contamed lc's a few weeks ago and that really fucked it up, but even when i used a fresh syringe, most of my jars got contamed. with the few fully colonized jars, i did a few g2g and then also spawned some. the ones i spawned are fine and fruiting right now somehow, but all of the g2g got contaminated.

So with my last fully colonized jar, i did a g2g. before hand i cleaned out my glove box with clorox bleach spray, than sprayed the ever living shit out of it with lysol a few times before i began. with those jars, i put in a cleaned tub with no holes, and still i'm seeing signs of contams. One jar is clear that it will get contaminated, and the others  are just starting to show it. i'm praying that it wont, but after all of this i'm getting to the point where i just expect it to get contaminated...

so now i noc'd up 7 jars with a fresh syringe of penis envy and are currently sitting in the same tub. its the only one i have right now with out holes, so im getting another probably tomorrow.

anywho, after 6 dozen contaminated jars, i'm fucking giving up. my first grow had no contams at all. now out of like 60 jars i only got 5... what do i need to do? i have a rug in my room, a lot of tapestry, clothes, and other cloth stuff. do i need to remove that and fully clean it? i keep most of the shit in my closet. should i get some painters tarp(the clear plastic kind) and line the room with that, in an attempt to make a clean room?

thanks for all the help. i just had the most magical night last night on 4 grams of aborts, and i cant wait to grow some more and spread the love ;D

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OfflineLifted1
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Re: what the fuck is happening [Re: Blake_Shroom]
    #16176738 - 05/03/12 01:49 PM (12 years, 8 months ago)

I had a similar experience as you. I ask you this: what is your jar/lid design ? How about your grain prep? Substrate method?


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Discovering yourself is only "discovery" in so much as you discovered that what you once thought was real, is only a mirage.

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InvisibleDoc_T
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Re: what the fuck is happening [Re: Blake_Shroom]
    #16176744 - 05/03/12 01:50 PM (12 years, 8 months ago)

Sounds like bad syringes. (If your grains are sterile to begin with)
Agar is the way to go, agar work is easier and cheaper than it looks.


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You make it all possible. Doesn't it feel good?

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Offlinecarnage11
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Re: what the fuck is happening [Re: Blake_Shroom]
    #16176758 - 05/03/12 01:53 PM (12 years, 8 months ago)

I would go through and clean, and I mean DETAIL clean everything in your house.  Sanitize with Lysol and vacuum and vacuum and vacuum some more.  Clean the ceiling fans, steam clean the carpets, and wipe every surface with a disinfectant wipe. Change your air filter and clean all the vents. Bleach the fuck out of your bathroom and turn it into a clean room.  Then turn the A/C off if it's running, turn off all fans, make sure you have still air and do all of your clean work in the bathroom, or whatever room you can make the cleanest.  I say bathroom because generally bathrooms are easy to bleach and sterilize.

I do all of my sterile work in open air, I don't use a glove box or flow hood, but my house is very clean and I even make sure to bleach all surfaces before any work is to be done and then I sanitize the air with Oust or something. Let the air settle and wear a mask if you can't hold your breath.


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You're breathing so I guess you're still alive
Even if signs seem to tell me otherwise.


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InvisibleDoc_T
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Re: what the fuck is happening [Re: carnage11]
    #16176767 - 05/03/12 01:56 PM (12 years, 8 months ago)

Easiest way to check that^^^ is to do a blank g2g.
Make some grain jars, but don't shoot anything into them.
G2G one into the others, then wait. If they contam, it's your procedure.
If they don't, your procedure is ok but your inoculant is bad.

Or, y'know, drop a drop on an agar dish.

(I'm an open-air guy too. Too claustrophobic for a glovebox and too cheap to buy a flowhood)


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You make it all possible. Doesn't it feel good?

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OfflineBlake_Shroom
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Re: what the fuck is happening [Re: Doc_T]
    #16176785 - 05/03/12 01:59 PM (12 years, 8 months ago)

I follow rr rye tek, pc for like 2 hours at 15 psi. i have jars that i pc'd a few weeks ago and they have no contams. i move out in august so i really dont want to go through all of that work to clean my room and such, should i just do that to my closet like i proposed?

and i have some fruiting happening now and some agar, so i'm gonna get a pin and drop it on agar just for now and see.

my real question is how does my fully colonized jar, with no contams, do a g2g and then get contamed? how does that work?

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OfflineLifted1
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Re: what the fuck is happening [Re: Blake_Shroom]
    #16176819 - 05/03/12 02:10 PM (12 years, 8 months ago)

Fully colonized jars can be contaminated


--------------------

Discovering yourself is only "discovery" in so much as you discovered that what you once thought was real, is only a mirage.

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Offlinecarnage11
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Re: what the fuck is happening [Re: Lifted1]
    #16176828 - 05/03/12 02:12 PM (12 years, 8 months ago)

Go down to the corner and buy a nice fat gram of Meth.  You'll have your house clean in no time. :thumbup:


--------------------
You're breathing so I guess you're still alive
Even if signs seem to tell me otherwise.


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InvisibleDoc_T
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Re: what the fuck is happening [Re: Blake_Shroom]
    #16176920 - 05/03/12 02:36 PM (12 years, 8 months ago)

Quote:

Blake_Shroom said:
my real question is how does my fully colonized jar, with no contams, do a g2g and then get contamed? how does that work?




Hidden contam. It happens, they aren't all virulent and aggressive, some are pretty subtle.
Or the jars weren't sterile to begin with or you messed up your g2g procedure. Hard to say really.

Drop that pin on an agar dish and go from there.


--------------------
You make it all possible. Doesn't it feel good?

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InvisibleThayendanegea
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Re: what the fuck is happening [Re: carnage11]
    #16177374 - 05/03/12 04:02 PM (12 years, 8 months ago)

Quote:

carnage11 said:
Go down to the corner and buy a nice fat gram of Meth.  You'll have your house clean in no time. :thumbup:



:foshizzle: LMFAO


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Look Deep Into Nature,and Then You Will Understand Everything Better.

Albert Einstein

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