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Offlineshroomer17
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Came across a live mycelium syringe, how best to use it??
    #16176798 - 05/03/12 02:05 PM (12 years, 8 months ago)

Need some advice here guys...

I was extremely lucky to find a head shop selling a syringe with a chunk of live mycelium in it, rather than just spores.  It was their last one and they aren't selling them any more due to the legality of the situation.  I want to treat this syringe like gold, because I haven't been able to get my hands on clean spores lately due to money issues.  Plus this is probably a good, isolated strain.

What would you do with it?

I have some agar powder, but no proper petri dishes, and next to no agar experience.

I can make up some LC, but am worried about contams being invisible.

I would like to do a grain LC, but I dont have any syringe filters to make the lid water tight for shaking.

I have lots of uninocculated bags ready to go, but dont really want to waste my syringe going straight to bags without doing something to preserve the culture.

I have no flowhood.  If i did, i'd definitely start some agar jars and some growbags and start doing lots of G2G's... I can make a glove box but thats about it.

Suggestions??

Edited by shroomer17 (05/03/12 02:05 PM)

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OfflineMOPE
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Re: Came across a live mycelium syringe, how best to use it?? [Re: shroomer17]
    #16176820 - 05/03/12 02:10 PM (12 years, 8 months ago)

you don't need a syringe filter for GLC.  Just shoot up a few half-filled half-pints, and when the grain is fully colonized put some duct tape over the GE hole and shake.

That would be my suggestion


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OfflineSoreSpore
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Re: Came across a live mycelium syringe, how best to use it?? [Re: MOPE] * 1
    #16176829 - 05/03/12 02:12 PM (12 years, 8 months ago)

How in the world is that headshop still open? :facepalm:

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OfflineSoreSpore
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Re: Came across a live mycelium syringe, how best to use it?? [Re: SoreSpore]
    #16176840 - 05/03/12 02:13 PM (12 years, 8 months ago)

Sorry, forgot to suggest as well. I'd make up as many LC's as you can and inject each one with 1cc of the syringe. Incubate until fully grown and then toss them in the fridge. They will last well over a year.

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InvisibleTranscendingLife
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Re: Came across a live mycelium syringe, how best to use it?? [Re: shroomer17]
    #16176849 - 05/03/12 02:15 PM (12 years, 8 months ago)

Make a glove box.
Inoculate 1 quart jar w/ 1cc of solution.
G2G that 1 jar when it's 100% to 8 quarts & 1 pint jar.
Use the 1 pint jar for a GLC & the 8 quarts for more G2Gs or spawn it to bulk.

:2cents:


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InvisibleDoc_T
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Re: Came across a live mycelium syringe, how best to use it?? [Re: TranscendingLife]
    #16176910 - 05/03/12 02:34 PM (12 years, 8 months ago)

Quote:

I have some agar powder, but no proper petri dishes, and next to no agar experience.




You can do agar in pf jars if you have them. They're big and bulky but it works fine.

I'd do what TL says too. :thumbup:


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Offlineshroomer17
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Re: Came across a live mycelium syringe, how best to use it?? [Re: TranscendingLife]
    #16176941 - 05/03/12 02:41 PM (12 years, 8 months ago)

Quote:

MOPE said:
you don't need a syringe filter for GLC.  Just shoot up a few half-filled half-pints, and when the grain is fully colonized put some duct tape over the GE hole and shake.

That would be my suggestion




Usually when I do LCs I use a fully sealed lid with 2 RTV ports on it, and when it comes time to use the solution, I stick a syringe stuffed with polyfil in one port and withdraw the LC into a syringe using the other port.  Think I can get away with a fully sealed lid if i use a small amount of grain in my half pint?  If not, what lid design would you suggest?

Quote:

TranscendingLife said:
Make a glove box.
Inoculate 1 quart jar w/ 1cc of solution.
G2G that 1 jar when it's 100% to 8 quarts & 1 pint jar.
Use the 1 pint jar for a GLC & the 8 quarts for more G2Gs or spawn it to bulk.

:2cents:




I dont really ever use jars because they dont produce enough spawn for my liking.  I basically always stick to bags.  I am also not comfortable doing G2G even in a glovebox because I think it would still be very prone to contamination.  Even if I did do G2G in the glovebox, it would be very cramped and difficult to work with bags in the glovebox.  All my innoculations are done in open air with a syringe condom style technique (sponge over needle).

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InvisibleTranscendingLife
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Re: Came across a live mycelium syringe, how best to use it?? [Re: shroomer17]
    #16176980 - 05/03/12 02:47 PM (12 years, 8 months ago)

I've done 100s of G2Gs in my SAB & gotten 1 contam.
I can do 1 jar to 12 w/ ease in under 10 minutes.
I can G2G 2 jars to 2 6 quart bags in there, too.
It's a 116L tote.


--------------------
AMU: We Quickly Answer Questions Here
"One must accept the probability of failure to experience the elation of success." - TranscendingLife
“A man of genius makes no mistakes; his errors are volitional and are the portals of discovery.” - James Joyce
:sporedrop::sporedrop::mushroomgrow::sporedrop::sporedrop::wow:How I Do EVERYTHING:wow::sporedrop::sporedrop::mushroomgrow::sporedrop::sporedrop:
"Your vision will become clear only when you can look into your own heart…. Who looks outside, dreams; who looks inside, awakes."- Carl Jung
"Anything that can be done chemically can be done by other means."- William S. Burroughs
"You are as dead now as you will ever be" - Seth

Edited by TranscendingLife (05/03/12 02:59 PM)

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