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OfflineProject
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How long until liquid culture inoculation starts to show growth?
    #16176642 - 05/03/12 01:27 PM (11 years, 8 months ago)

I inoculated some pint jars yesterday with between 1 and 1.5cc of LC and a quart jar with 2cc.

When should I expect to start seeing signs of mycelial growth?


Edited by Project (05/03/12 01:29 PM)


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OfflineWing
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Re: How long until liquid culture inoculation starts to show growth? [Re: Project]
    #16176681 - 05/03/12 01:36 PM (11 years, 8 months ago)

Usually within the first couple days


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InvisibleDynGBreeD
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Re: How long until liquid culture inoculation starts to show growth? [Re: Project] * 1
    #16176690 - 05/03/12 01:38 PM (11 years, 8 months ago)

Depends... I usually get growth between 4-7 days..... Honestly if you made LC with a MS syringe it only speeds up growth upon inoculation about maybe a 5-7 days. It's almost pointless with MS unless you have made a Clone/Isolate.


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OfflineProject
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Re: How long until liquid culture inoculation starts to show growth? [Re: DynGBreeD]
    #16176697 - 05/03/12 01:39 PM (11 years, 8 months ago)

Well, 5-7 days is something..  Hardly pointless! 

:jiggletits:


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OfflineWing
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Re: How long until liquid culture inoculation starts to show growth? [Re: Project]
    #16176718 - 05/03/12 01:45 PM (11 years, 8 months ago)

It's nice to make your LC like a week or so before you plan to get started so you're injecting already growing mycelium instead of waiting for spores to germinate.

A clone or iso LC is better though like DynGBreeD said.


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InvisibleDoc_T
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Re: How long until liquid culture inoculation starts to show growth? [Re: Wing]
    #16176728 - 05/03/12 01:47 PM (11 years, 8 months ago)

I used to be a big LC fan... then I realized how pointless it is.
5-7 days isn't the issue. Spores->agar->grain->g2g gets you more spawn than you can use in less time than you'd believe.
And it's clean. Which saves 5-7 days easily.


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OfflineSporeAddict
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Re: How long until liquid culture inoculation starts to show growth? [Re: Project] * 1
    #16176743 - 05/03/12 01:50 PM (11 years, 8 months ago)

Dear Peeps.
  I'm uncertain where the best place to ask this is , but maybe y'all can help.I'm a new grower and am preparing to start my second grow. I intend to  do a liquids culture,and I plan to grow to horse poo.My question is;  Should I inocculate brf jars with the LC or Can i go right to the hpoo with the LC?


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InvisibleDoc_T
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Re: How long until liquid culture inoculation starts to show growth? [Re: SporeAddict]
    #16176752 - 05/03/12 01:52 PM (11 years, 8 months ago)

1) don't make LC
2) do make brf jars
3) don't spawn them to poo
4) do fruit them as cakes in a shotgun FC, as shown in Roger Rabbit's videos: http://www.mushroomvideos.com/


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OfflineProject
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Re: How long until liquid culture inoculation starts to show growth? [Re: Doc_T]
    #16176786 - 05/03/12 02:00 PM (11 years, 8 months ago)

Or you could check out the Horse Poo Tek in my signature.

I would have done a bulk grow for my first time instead of PF tek if information was better organized on this forum.  Sometimes you ask one question and get lots of different answers.  All of the information is here to make it so easy, it's just very fragmented.

As far as what I'm doing, I would love to work with agar and get an isolate.  Thing is, my first grow hasn't even been harvested yet.  If I get some nice mushroom, I'll try to isolate it and then go to G2G.  And speaking of G2G, when my next round of jars finishes, I'll probably use a few for G2G with MS genetics.  But the iso is definitely what I'm after.

I've got a small monotub and a bulk try colonizing at the moment and waiting 18.5 quarts worth of spawn...  It's with a few of those pint jars I'll do some G2G.  Then if I get a good mushroom for an isolate, I'll get to working on that.

I already have plenty of spawn on it's way, I'm in no hurry.


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OfflineSporeAddict
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Re: How long until liquid culture inoculation starts to show growth? [Re: Project]
    #16176810 - 05/03/12 02:07 PM (11 years, 8 months ago)

Uncertain of what a Shotgun FC is


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Offlinezepski
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Re: How long until liquid culture inoculation starts to show growth? [Re: SporeAddict]
    #16176886 - 05/03/12 02:26 PM (11 years, 8 months ago)

Quote:

SporeAddict said:
Uncertain of what a Shotgun FC is




Roger Rabbit's videos: http://www.mushroomvideos.com/&nbsp; <------- watch the videos


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InvisibleDoc_T
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Re: How long until liquid culture inoculation starts to show growth? [Re: Project]
    #16176946 - 05/03/12 02:43 PM (11 years, 8 months ago)

Quote:

Project said:
I would have done a bulk grow for my first time instead of PF tek if information was better organized on this forum. 




Really? I would have done cakes instead of grains for my first grow if I knew then what I know now.


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OfflineSporeAddict
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Re: How long until liquid culture inoculation starts to show growth? [Re: Doc_T]
    #16177043 - 05/03/12 02:59 PM (11 years, 8 months ago)

Ok , if the SCFC is the tote with  the saturated pearlite, then that's how  i did my first grow. I was wanting to streach my spores out. I dont have prints stockpiled yet


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OfflineRevolutiontcm
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Re: How long until liquid culture inoculation starts to show growth? [Re: SporeAddict]
    #16177682 - 05/03/12 05:27 PM (11 years, 8 months ago)

Made 3 half pint karo lc's on the 30th knocked with ms first specs seen today


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OfflineProject
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Re: How long until liquid culture inoculation starts to show growth? [Re: Doc_T]
    #16177803 - 05/03/12 05:54 PM (11 years, 8 months ago)

Quote:

Doc_T said:
Quote:

Project said:
I would have done a bulk grow for my first time instead of PF tek if information was better organized on this forum. 




Really? I would have done cakes instead of grains for my first grow if I knew then what I know now.




Why?


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InvisibleDoc_T
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Re: How long until liquid culture inoculation starts to show growth? [Re: Project]
    #16177949 - 05/03/12 06:28 PM (11 years, 8 months ago)

Cakes are the superior choice for a first grow for many reasons.
Yield is excellent, ingredients are plentiful. But for me, the top reason is modularity.
If a cake goes bad, you toss it and fruit the other 11.
If a jar of spawn was bad, your tub is bad. You lose it, the materials and time it took, and possibly spread contams around.
Plus cakes are much, much faster from needle to harvest than any other method.


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OfflineWing
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Re: How long until liquid culture inoculation starts to show growth? [Re: Doc_T]
    #16180727 - 05/04/12 08:05 AM (11 years, 8 months ago)

Yep. Cakes are so underrated. Best way to grow out and test isolates. Best way for beginners to learn the craft without putting all their eggs in one basket. And once you've done them a few times it's really easy and quick to make up a batch.


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InvisibleHorizonSpawn
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Re: How long until liquid culture inoculation starts to show growth? [Re: Wing]
    #16180772 - 05/04/12 08:24 AM (11 years, 8 months ago)

:whathesaid:


Also; I would never ever recommend inoculating a nutritive liquid culture with spores :facepalm:  Perhaps a grain from a master grain jar, or a clean tissue transfer from a fruitbody; or for best and most reliable results, a transfer from a clean culture grown out on agar :thumbup:


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Please assume any and all prints exchanged are "WILD" in nature; and thus, should NOT be considered ASEPTIC...

NOTE:  Please excuse my brevity, as it is a bitch 'n' a half and slow as hell to type on this here phone :frown:


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OfflineProject
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Re: How long until liquid culture inoculation starts to show growth? [Re: Wing]
    #16182631 - 05/04/12 05:20 PM (11 years, 8 months ago)

Quote:

Wing said:
Yep. Cakes are so underrated. Best way to grow out and test isolates. Best way for beginners to learn the craft without putting all their eggs in one basket. And once you've done them a few times it's really easy and quick to make up a batch.




Yeah, that makes sense.

I'm looking forward to getting my first isolate soon, need to read up on some stuff.

Does anyone have any good links or teks they can throw my way?  I'd really appreciate it :smile:


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OfflineProject
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Re: How long until liquid culture inoculation starts to show growth? [Re: Project]
    #16182638 - 05/04/12 05:23 PM (11 years, 8 months ago)

The method I used to make my LC is agar's tek, the one in my signature.  Seems like a good way to go if you can avoid contamination.

I'm starting to see what I think it the first signs of growth, now I'm just going to have to wait to see what happens next.


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