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Project
Ride the Spiral


Registered: 06/02/09
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Last seen: 11 years, 21 days
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How long until liquid culture inoculation starts to show growth?
#16176642 - 05/03/12 01:27 PM (11 years, 8 months ago) |
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I inoculated some pint jars yesterday with between 1 and 1.5cc of LC and a quart jar with 2cc.
When should I expect to start seeing signs of mycelial growth?
Edited by Project (05/03/12 01:29 PM)
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Wing
The Eye Tyrant



Registered: 04/25/11
Posts: 3,293
Last seen: 5 months, 9 days
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Re: How long until liquid culture inoculation starts to show growth? [Re: Project]
#16176681 - 05/03/12 01:36 PM (11 years, 8 months ago) |
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Usually within the first couple days
-------------------- My Old Grow Logs
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DynGBreeD
┌∩┐(◣_◢)┌∩┐

Registered: 01/15/11
Posts: 3,639
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Re: How long until liquid culture inoculation starts to show growth? [Re: Project] 1
#16176690 - 05/03/12 01:38 PM (11 years, 8 months ago) |
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Depends... I usually get growth between 4-7 days..... Honestly if you made LC with a MS syringe it only speeds up growth upon inoculation about maybe a 5-7 days. It's almost pointless with MS unless you have made a Clone/Isolate.
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Project
Ride the Spiral


Registered: 06/02/09
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Re: How long until liquid culture inoculation starts to show growth? [Re: DynGBreeD]
#16176697 - 05/03/12 01:39 PM (11 years, 8 months ago) |
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Well, 5-7 days is something.. Hardly pointless!
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Wing
The Eye Tyrant



Registered: 04/25/11
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Re: How long until liquid culture inoculation starts to show growth? [Re: Project]
#16176718 - 05/03/12 01:45 PM (11 years, 8 months ago) |
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It's nice to make your LC like a week or so before you plan to get started so you're injecting already growing mycelium instead of waiting for spores to germinate.
A clone or iso LC is better though like DynGBreeD said.
-------------------- My Old Grow Logs
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Doc_T
Random Dude




Registered: 03/06/09
Posts: 42,395
Loc: Colorado
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Re: How long until liquid culture inoculation starts to show growth? [Re: Wing]
#16176728 - 05/03/12 01:47 PM (11 years, 8 months ago) |
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I used to be a big LC fan... then I realized how pointless it is. 5-7 days isn't the issue. Spores->agar->grain->g2g gets you more spawn than you can use in less time than you'd believe. And it's clean. Which saves 5-7 days easily.
-------------------- You make it all possible. Doesn't it feel good?
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SporeAddict
Sporadic



Registered: 04/30/12
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Re: How long until liquid culture inoculation starts to show growth? [Re: Project] 1
#16176743 - 05/03/12 01:50 PM (11 years, 8 months ago) |
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Dear Peeps. I'm uncertain where the best place to ask this is , but maybe y'all can help.I'm a new grower and am preparing to start my second grow. I intend to do a liquids culture,and I plan to grow to horse poo.My question is; Should I inocculate brf jars with the LC or Can i go right to the hpoo with the LC?
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Doc_T
Random Dude




Registered: 03/06/09
Posts: 42,395
Loc: Colorado
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Re: How long until liquid culture inoculation starts to show growth? [Re: SporeAddict]
#16176752 - 05/03/12 01:52 PM (11 years, 8 months ago) |
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1) don't make LC 2) do make brf jars 3) don't spawn them to poo 4) do fruit them as cakes in a shotgun FC, as shown in Roger Rabbit's videos: http://www.mushroomvideos.com/
-------------------- You make it all possible. Doesn't it feel good?
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Project
Ride the Spiral


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Re: How long until liquid culture inoculation starts to show growth? [Re: Doc_T]
#16176786 - 05/03/12 02:00 PM (11 years, 8 months ago) |
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Or you could check out the Horse Poo Tek in my signature.
I would have done a bulk grow for my first time instead of PF tek if information was better organized on this forum. Sometimes you ask one question and get lots of different answers. All of the information is here to make it so easy, it's just very fragmented.
As far as what I'm doing, I would love to work with agar and get an isolate. Thing is, my first grow hasn't even been harvested yet. If I get some nice mushroom, I'll try to isolate it and then go to G2G. And speaking of G2G, when my next round of jars finishes, I'll probably use a few for G2G with MS genetics. But the iso is definitely what I'm after.
I've got a small monotub and a bulk try colonizing at the moment and waiting 18.5 quarts worth of spawn... It's with a few of those pint jars I'll do some G2G. Then if I get a good mushroom for an isolate, I'll get to working on that.
I already have plenty of spawn on it's way, I'm in no hurry.
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SporeAddict
Sporadic



Registered: 04/30/12
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Re: How long until liquid culture inoculation starts to show growth? [Re: Project]
#16176810 - 05/03/12 02:07 PM (11 years, 8 months ago) |
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Uncertain of what a Shotgun FC is
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zepski
Ya Momma



Registered: 10/01/11
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Re: How long until liquid culture inoculation starts to show growth? [Re: SporeAddict]
#16176886 - 05/03/12 02:26 PM (11 years, 8 months ago) |
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Quote:
SporeAddict said: Uncertain of what a Shotgun FC is
Roger Rabbit's videos: http://www.mushroomvideos.com/ <------- watch the videos
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Doc_T
Random Dude




Registered: 03/06/09
Posts: 42,395
Loc: Colorado
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Re: How long until liquid culture inoculation starts to show growth? [Re: Project]
#16176946 - 05/03/12 02:43 PM (11 years, 8 months ago) |
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Quote:
Project said: I would have done a bulk grow for my first time instead of PF tek if information was better organized on this forum.
Really? I would have done cakes instead of grains for my first grow if I knew then what I know now.
-------------------- You make it all possible. Doesn't it feel good?
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SporeAddict
Sporadic



Registered: 04/30/12
Posts: 28
Loc: Arkansas
Last seen: 10 years, 7 months
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Re: How long until liquid culture inoculation starts to show growth? [Re: Doc_T]
#16177043 - 05/03/12 02:59 PM (11 years, 8 months ago) |
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Ok , if the SCFC is the tote with the saturated pearlite, then that's how i did my first grow. I was wanting to streach my spores out. I dont have prints stockpiled yet
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Revolutiontcm
dniM-retsaM



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Re: How long until liquid culture inoculation starts to show growth? [Re: SporeAddict]
#16177682 - 05/03/12 05:27 PM (11 years, 8 months ago) |
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Made 3 half pint karo lc's on the 30th knocked with ms first specs seen today
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Project
Ride the Spiral


Registered: 06/02/09
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Re: How long until liquid culture inoculation starts to show growth? [Re: Doc_T]
#16177803 - 05/03/12 05:54 PM (11 years, 8 months ago) |
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Quote:
Doc_T said:
Quote:
Project said: I would have done a bulk grow for my first time instead of PF tek if information was better organized on this forum.
Really? I would have done cakes instead of grains for my first grow if I knew then what I know now.
Why?
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Doc_T
Random Dude




Registered: 03/06/09
Posts: 42,395
Loc: Colorado
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Re: How long until liquid culture inoculation starts to show growth? [Re: Project]
#16177949 - 05/03/12 06:28 PM (11 years, 8 months ago) |
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Cakes are the superior choice for a first grow for many reasons. Yield is excellent, ingredients are plentiful. But for me, the top reason is modularity. If a cake goes bad, you toss it and fruit the other 11. If a jar of spawn was bad, your tub is bad. You lose it, the materials and time it took, and possibly spread contams around. Plus cakes are much, much faster from needle to harvest than any other method.
-------------------- You make it all possible. Doesn't it feel good?
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Wing
The Eye Tyrant



Registered: 04/25/11
Posts: 3,293
Last seen: 5 months, 9 days
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Re: How long until liquid culture inoculation starts to show growth? [Re: Doc_T]
#16180727 - 05/04/12 08:05 AM (11 years, 8 months ago) |
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Yep. Cakes are so underrated. Best way to grow out and test isolates. Best way for beginners to learn the craft without putting all their eggs in one basket. And once you've done them a few times it's really easy and quick to make up a batch.
-------------------- My Old Grow Logs
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HorizonSpawn
Gettin' my grow on :)


Registered: 05/14/11
Posts: 1,739
Loc: Myco-Tek.org
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Re: How long until liquid culture inoculation starts to show growth? [Re: Wing]
#16180772 - 05/04/12 08:24 AM (11 years, 8 months ago) |
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Also; I would never ever recommend inoculating a nutritive liquid culture with spores Perhaps a grain from a master grain jar, or a clean tissue transfer from a fruitbody; or for best and most reliable results, a transfer from a clean culture grown out on agar
-------------------- Please assume any and all prints exchanged are "WILD" in nature; and thus, should NOT be considered ASEPTIC... NOTE: Please excuse my brevity, as it is a bitch 'n' a half and slow as hell to type on this here phone
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Project
Ride the Spiral


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Re: How long until liquid culture inoculation starts to show growth? [Re: Wing]
#16182631 - 05/04/12 05:20 PM (11 years, 8 months ago) |
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Quote:
Wing said: Yep. Cakes are so underrated. Best way to grow out and test isolates. Best way for beginners to learn the craft without putting all their eggs in one basket. And once you've done them a few times it's really easy and quick to make up a batch.
Yeah, that makes sense.
I'm looking forward to getting my first isolate soon, need to read up on some stuff.
Does anyone have any good links or teks they can throw my way? I'd really appreciate it
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Project
Ride the Spiral


Registered: 06/02/09
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Re: How long until liquid culture inoculation starts to show growth? [Re: Project]
#16182638 - 05/04/12 05:23 PM (11 years, 8 months ago) |
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The method I used to make my LC is agar's tek, the one in my signature. Seems like a good way to go if you can avoid contamination.
I'm starting to see what I think it the first signs of growth, now I'm just going to have to wait to see what happens next.
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Doc_T
Random Dude




Registered: 03/06/09
Posts: 42,395
Loc: Colorado
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Re: How long until liquid culture inoculation starts to show growth? [Re: Project] 1
#16182739 - 05/04/12 05:52 PM (11 years, 8 months ago) |
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Quote:
Project said: Seems like a good way to go if you can avoid contamination.
You can't. Not if you're using spores. You might get lucky, might not. But you can't control it.
Agar technique, on the other hand, gives you complete control over contamination. Grain LC is better than sugar LC from spores. But if the syringe isn't quite clean, your GLC can have a subtle contaminant.
Agar. It's where you want to be.
-------------------- You make it all possible. Doesn't it feel good?
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mental-floss
FUN-GUY


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Re: How long until liquid culture inoculation starts to show growth? [Re: Wing]
#22154036 - 08/27/15 09:53 PM (8 years, 5 months ago) |
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How long should I expect my halo pints to colonize there not showing signs and they were inoculated with syringe about a week ago ?!? I love the of tech it's been a while and I forgot how long it takes I used about 1 cc each hole times for wholes so four cc's each jar so two jars per syringe maybe that's the problem or do they need air exchange ?!?!
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Skillzd
Stranger
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Re: How long until liquid culture inoculation starts to show growth? [Re: Project]
#23391574 - 06/28/16 04:39 PM (7 years, 6 months ago) |
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Quote:
Project said: Or you could check out the Horse Poo Tek in my signature.
I would have done a bulk grow for my first time instead of PF tek if information was better organized on this forum. Sometimes you ask one question and get lots of different answers. All of the information is here to make it so easy, it's just very fragmented.
As far as what I'm doing, I would love to work with agar and get an isolate. Thing is, my first grow hasn't even been harvested yet. If I get some nice mushroom, I'll try to isolate it and then go to G2G. And speaking of G2G, when my next round of jars finishes, I'll probably use a few for G2G with MS genetics. But the iso is definitely what I'm after.
I've got a small monotub and a bulk try colonizing at the moment and waiting 18.5 quarts worth of spawn... It's with a few of those pint jars I'll do some G2G. Then if I get a good mushroom for an isolate, I'll get to working on that.
I already have plenty of spawn on it's way, I'm in no hurry.
I like you. Lol. Too many people give random and different answers to the same question. Or ya got the guys who act like they hate helping us newbies cus it's always the same stuff over and over again. Well of course. If you have 100 issues that can go wrong. It's always going to be ONE OR TWO of those 100 issues. Repeatedly That's common sense that if you help people on here longterm. It's about to start repeating itself. Lol. And the worst is when I ask a question about my mess up and right away they start. WHY DIDNT YOU FOLLOW THE TEK TO THE "T". Obviously I either made a MISTAKE and missed something. Or I wouldn't be here with a problem. Right? And then secondly. Maybe I WANT AMD ENJOY experiments. And maybe I WANT to know what Contams look like so I don't mind failures on the side where it doesn't effect my others. If that's what I enjoy and WANT to do this time. Then so be it. But it seems more people are worried about WHY. Instead of just answering a question to help. I try to talk and make friends to chat with. Not just use you for help also. So if I chat about other things beside growing. Either chat back. Or ignore it. I had someone bitch at me for typing stuff not necessary to the question. Soooo. I wanted to chat too. Lol. But I love it when someone like you actually enjoys helping and likes to chat here too
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Inocuole
Scalpel of Evil's Bane



Registered: 11/21/11
Posts: 24,863
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Re: How long until liquid culture inoculation starts to show growth? [Re: Skillzd]
#23391644 - 06/28/16 05:06 PM (7 years, 6 months ago) |
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Quote:
Skillzd said:
I like you. Lol. Too many people give random and different answers to the same question. Or ya got the guys who act like they hate helping us newbies cus it's always the same stuff over and over again. Well of course. If you have 100 issues that can go wrong. It's always going to be ONE OR TWO of those 100 issues. Repeatedly That's common sense that if you help people on here longterm. It's about to start repeating itself. Lol. And the worst is when I ask a question about my mess up and right away they start. WHY DIDNT YOU FOLLOW THE TEK TO THE "T". Obviously I either made a MISTAKE and missed something. Or I wouldn't be here with a problem. Right? And then secondly. Maybe I WANT AMD ENJOY experiments. And maybe I WANT to know what Contams look like so I don't mind failures on the side where it doesn't effect my others. If that's what I enjoy and WANT to do this time. Then so be it. But it seems more people are worried about WHY. Instead of just answering a question to help. I try to talk and make friends to chat with. Not just use you for help also. So if I chat about other things beside growing. Either chat back. Or ignore it. I had someone bitch at me for typing stuff not necessary to the question. Soooo. I wanted to chat too. Lol. But I love it when someone like you actually enjoys helping and likes to chat here too
Wow... quite a ramble there.
4 year old thread dude.

Furthermore, growing contams purposefully so you "know what they look like" is fucking dumb. Wasting time and materials in the age of infinite information is a pitiful shame. You also sound underage. 
Also OP was "Last seen: 3 years, 5 months ". So, he probably won't want to chat.
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Skillzd
Stranger
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Re: How long until liquid culture inoculation starts to show growth? [Re: Inocuole]
#23392174 - 06/28/16 08:29 PM (7 years, 6 months ago) |
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Yeah sorry. I honestly missed the date on it. But yeah I just don't see to many people calling it like it is on here and not even seeming like they like being here. Saying you do if not the same as the way the posts sound and just sounds like you hate helping anymore. There's a ton of those here. Sorry for the ramble. I needed to vent I guess. Hava
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Skillzd
Stranger
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Re: How long until liquid culture inoculation starts to show growth? [Re: Skillzd]
#23392177 - 06/28/16 08:30 PM (7 years, 6 months ago) |
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Oh and I wasnt seriously saying I was purposely growing them to see what they look like. I just simply meant it Helps to know what they look like. Sorry for the confusion there. Lol. And no I'm not underage. Just a bit tired of the one question and 12 different answers that you so often see
And the many MAYBEs were not to take it literal. I was just more ticked off at some things that people are so quick to judge. But I do also like to experiment with extra stuff on the side seperate from my actuall grow I started. Certain thingas that are still useful to know
Edited by Skillzd (06/28/16 08:46 PM)
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Inocuole
Scalpel of Evil's Bane



Registered: 11/21/11
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Re: How long until liquid culture inoculation starts to show growth? [Re: Skillzd]
#23392195 - 06/28/16 08:37 PM (7 years, 6 months ago) |
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Well, venting your rage about some of the more callous personalities here (ahem..) randomly in old threads using disjointed paragraphs is liable to uh.. draw attention, you might say.
Something important to remember is that you cannot fully judge how a person in a certain position acts because you do not know what it's like to be that person. There will be unseen key information about the motives of that person that could change how the situation appears, with no way for you to know of it.
Frustration begets change. And resisting change is how you have a bad time, if you know anything about psychedelics.
If you think the people who are tired of certain questions are unfounded in being frustrated, either get to their level without becoming as callous to prove it's possible, or humor them for 30 seconds and consider why they feel the way they do, possibly empowering yourself in the process.
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Skillzd
Stranger
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Re: How long until liquid culture inoculation starts to show growth? [Re: Inocuole]
#23392238 - 06/28/16 08:53 PM (7 years, 6 months ago) |
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Very smart. See you are the kind of person I could enjoy chatting with. Honestly I do understand why certain questions and repeat issues get the response they do. But like you said. You also can't judge the others situation because you don't know it. Maybe someone only had 5min on a computer and figured they would have better luck asking here and then next time they get a minute to check the response.
Everyone has a different way of thinking and/or has different ways of doing things. And yeah like I said I honestly didn't see this was an old forum. When I had clicked on the link for it I thought it had a post from today.so I thought it was a newer one
And yes I have some issues I'm trying to deal with and need the actives to help with that. Life has a way of making me into a person I don't like and I need to get back in touch with myself and my spiritual side. I've used them before for this and just think others can benifit also.
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Skillzd
Stranger
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Re: How long until liquid culture inoculation starts to show growth? [Re: Skillzd]
#23392241 - 06/28/16 08:54 PM (7 years, 6 months ago) |
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Very nice tea recipe too. I can't wait to try it
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