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OfflineProject
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How long until liquid culture inoculation starts to show growth?
    #16176642 - 05/03/12 01:27 PM (11 years, 8 months ago)

I inoculated some pint jars yesterday with between 1 and 1.5cc of LC and a quart jar with 2cc.

When should I expect to start seeing signs of mycelial growth?


Edited by Project (05/03/12 01:29 PM)


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OfflineProject
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Re: How long until liquid culture inoculation starts to show growth? [Re: DynGBreeD]
    #16176697 - 05/03/12 01:39 PM (11 years, 8 months ago)

Well, 5-7 days is something..  Hardly pointless! 

:jiggletits:


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Re: How long until liquid culture inoculation starts to show growth? [Re: Doc_T]
    #16176786 - 05/03/12 02:00 PM (11 years, 8 months ago)

Or you could check out the Horse Poo Tek in my signature.

I would have done a bulk grow for my first time instead of PF tek if information was better organized on this forum.  Sometimes you ask one question and get lots of different answers.  All of the information is here to make it so easy, it's just very fragmented.

As far as what I'm doing, I would love to work with agar and get an isolate.  Thing is, my first grow hasn't even been harvested yet.  If I get some nice mushroom, I'll try to isolate it and then go to G2G.  And speaking of G2G, when my next round of jars finishes, I'll probably use a few for G2G with MS genetics.  But the iso is definitely what I'm after.

I've got a small monotub and a bulk try colonizing at the moment and waiting 18.5 quarts worth of spawn...  It's with a few of those pint jars I'll do some G2G.  Then if I get a good mushroom for an isolate, I'll get to working on that.

I already have plenty of spawn on it's way, I'm in no hurry.


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Re: How long until liquid culture inoculation starts to show growth? [Re: Doc_T]
    #16177803 - 05/03/12 05:54 PM (11 years, 8 months ago)

Quote:

Doc_T said:
Quote:

Project said:
I would have done a bulk grow for my first time instead of PF tek if information was better organized on this forum. 




Really? I would have done cakes instead of grains for my first grow if I knew then what I know now.




Why?


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Re: How long until liquid culture inoculation starts to show growth? [Re: Wing]
    #16182631 - 05/04/12 05:20 PM (11 years, 8 months ago)

Quote:

Wing said:
Yep. Cakes are so underrated. Best way to grow out and test isolates. Best way for beginners to learn the craft without putting all their eggs in one basket. And once you've done them a few times it's really easy and quick to make up a batch.




Yeah, that makes sense.

I'm looking forward to getting my first isolate soon, need to read up on some stuff.

Does anyone have any good links or teks they can throw my way?  I'd really appreciate it :smile:


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Re: How long until liquid culture inoculation starts to show growth? [Re: Project]
    #16182638 - 05/04/12 05:23 PM (11 years, 8 months ago)

The method I used to make my LC is agar's tek, the one in my signature.  Seems like a good way to go if you can avoid contamination.

I'm starting to see what I think it the first signs of growth, now I'm just going to have to wait to see what happens next.


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