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Riseup2day
Stranger

Registered: 03/21/12
Posts: 108
Last seen: 12 years, 7 months
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Re: Accidental LC? [Re: BaneInc]
#16163825 - 05/01/12 01:18 AM (12 years, 8 months ago) |
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Quote:
BaneInc said: Influence, is there an extended version of the syringe method you were describing?
http://www.shroomery.org/forums/showflat.php/Number/15201763#15201763
That's the whole tek to make spore syringes, but in a nutshell to sterilize the syringe:
1) Bring a pot of water to a rolling boil 2) Take empty syringe and suck up boiling water 3) Empty the water you sucked up into a sink (do not empty it back into the pot, treat the expelled water as dirty) 4) Repeat steps 2&3 5 times 5) On the last draw leave the water in the syringe and cap it.
Wait for it to cool and you can expect the syringe and the water to be sterile.
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BaneInc
Freshie


Registered: 04/29/12
Posts: 26
Loc: 48.8742° N, 2.3470° E
Last seen: 12 years, 7 months
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oh, perfect. Thanks Riseup! Much appreciated.
-------------------- - Bane
"Dreams are more real than reality itself, they're closer to the self."
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Galidor4
Beholder



Registered: 10/10/11
Posts: 854
Last seen: 6 years, 5 months
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Re: Accidental LC? [Re: BaneInc]
#16169026 - 05/02/12 12:11 AM (12 years, 8 months ago) |
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My question is: what next? How do I get the water from this bag into a cake or something?
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The Influence
Free Sheeks



Registered: 03/30/11
Posts: 6,067
Loc: Not Wisconsin
Last seen: 2 months, 19 hours
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Re: Accidental LC? [Re: Galidor4]
#16169042 - 05/02/12 12:15 AM (12 years, 8 months ago) |
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Quote:
Galidor4 said: My question is: what next? How do I get the water from this bag into a cake or something?
The only thing I would suggest is to use another syringe man. Unless you start working with agar I don't see many viable options.
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Riseup2day
Stranger

Registered: 03/21/12
Posts: 108
Last seen: 12 years, 7 months
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should've put that print into a jar instead of a syringe with a proper lid, then you can flame sterilize syringes and would be MUCH safer.
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BaneInc
Freshie


Registered: 04/29/12
Posts: 26
Loc: 48.8742° N, 2.3470° E
Last seen: 12 years, 7 months
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Yea Galidor, I agree with Influence. Your best bet would be to use a syringe and inoculate the best of it before the spores lose viability or get contaminated. I am not to sure how well that bag will keep it fresh and clean, but next time I would suggest using the method described by The Influence and linked to me from Riseup. Quote:
Riseup2day said:
Quote:
BaneInc said: Influence, is there an extended version of the syringe method you were describing?
http://www.shroomery.org/forums/showflat.php/Number/15201763#15201763
That's the whole tek to make spore syringes, but in a nutshell to sterilize the syringe:
1) Bring a pot of water to a rolling boil 2) Take empty syringe and suck up boiling water 3) Empty the water you sucked up into a sink (do not empty it back into the pot, treat the expelled water as dirty) 4) Repeat steps 2&3 5 times 5) On the last draw leave the water in the syringe and cap it.
Wait for it to cool and you can expect the syringe and the water to be sterile.
It seems to be a tried and tested method for success. i will be attempting my first shot at it this evening. Best of luck and let us know your results!
-------------------- - Bane
"Dreams are more real than reality itself, they're closer to the self."
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Galidor4
Beholder



Registered: 10/10/11
Posts: 854
Last seen: 6 years, 5 months
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Re: Accidental LC? [Re: BaneInc]
#16170777 - 05/02/12 11:44 AM (12 years, 8 months ago) |
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Apparently this wasn't well understood. This is not the way that I get spore syringes. I have a pretty good method going, that doesn't involve that much water or a bag. That bag, it was an accident, not something I planned on. But it seems like I'll just have to syringe it up(which I know how to do). i was just wondering if there was a different method to transferring the liquid to substrate.
I know what I'm doing on Friday.
Edited by Galidor4 (05/02/12 11:45 AM)
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Galidor4
Beholder



Registered: 10/10/11
Posts: 854
Last seen: 6 years, 5 months
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Re: Accidental LC? [Re: Galidor4]
#16171719 - 05/02/12 03:00 PM (12 years, 8 months ago) |
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If anyone here wants to see what I'm gonna do with that bag here's a link: Experiment #2
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BaneInc
Freshie


Registered: 04/29/12
Posts: 26
Loc: 48.8742° N, 2.3470° E
Last seen: 12 years, 7 months
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Re: Accidental LC? [Re: Galidor4]
#16172023 - 05/02/12 04:19 PM (12 years, 8 months ago) |
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Oh an accidental experiment! I am all about that! Im interested to see what kind of colonization you will get with that method.
-------------------- - Bane
"Dreams are more real than reality itself, they're closer to the self."
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Galidor4
Beholder



Registered: 10/10/11
Posts: 854
Last seen: 6 years, 5 months
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Re: Accidental LC? [Re: BaneInc]
#16173447 - 05/02/12 08:26 PM (12 years, 8 months ago) |
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Me too
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