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OfflineRiseup2day
Stranger

Registered: 03/21/12
Posts: 108
Last seen: 12 years, 7 months
Re: Accidental LC? [Re: BaneInc]
    #16163825 - 05/01/12 01:18 AM (12 years, 8 months ago)

Quote:

BaneInc said:
Influence, is there an extended version of the syringe method you were describing?





http://www.shroomery.org/forums/showflat.php/Number/15201763#15201763

That's the whole tek to make spore syringes, but in a nutshell to sterilize the syringe:

1) Bring a pot of water to a rolling boil
2) Take empty syringe and suck up boiling water
3) Empty the water you sucked up into a sink (do not empty it back into the pot, treat the expelled water as dirty)
4) Repeat steps 2&3 5 times
5) On the last draw leave the water in the syringe and cap it.


Wait for it to cool and you can expect the syringe and the water to be sterile.

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OfflineBaneInc
Freshie
Male

Registered: 04/29/12
Posts: 26
Loc: 48.8742° N, 2.3470° E
Last seen: 12 years, 7 months
Re: Accidental LC? [Re: Riseup2day]
    #16163838 - 05/01/12 01:22 AM (12 years, 8 months ago)

oh, perfect. Thanks Riseup! Much appreciated.


--------------------
- Bane

"Dreams are more real than reality itself, they're closer to the self."

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OfflineGalidor4
Beholder
Male


Registered: 10/10/11
Posts: 854
Last seen: 6 years, 5 months
Re: Accidental LC? [Re: BaneInc]
    #16169026 - 05/02/12 12:11 AM (12 years, 8 months ago)

My question is: what next? How do I get the water from this bag into a cake or something?

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OfflineThe Influence
Free Sheeks
Male User Gallery


Registered: 03/30/11
Posts: 6,067
Loc: Not Wisconsin
Last seen: 2 months, 19 hours
Re: Accidental LC? [Re: Galidor4]
    #16169042 - 05/02/12 12:15 AM (12 years, 8 months ago)

Quote:

Galidor4 said:
My question is: what next? How do I get the water from this bag into a cake or something?



The only thing I would suggest is to use another syringe man. Unless you start working with agar I don't see many viable options.

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OfflineRiseup2day
Stranger

Registered: 03/21/12
Posts: 108
Last seen: 12 years, 7 months
Re: Accidental LC? [Re: The Influence]
    #16169339 - 05/02/12 01:54 AM (12 years, 8 months ago)

should've put that print into a jar instead of a syringe with a proper lid, then you can flame sterilize syringes and would be MUCH safer.

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OfflineBaneInc
Freshie
Male

Registered: 04/29/12
Posts: 26
Loc: 48.8742° N, 2.3470° E
Last seen: 12 years, 7 months
Re: Accidental LC? [Re: Riseup2day]
    #16170374 - 05/02/12 10:20 AM (12 years, 8 months ago)

Yea Galidor, I agree with Influence. Your best bet would be to use a syringe and inoculate the best of it before the spores lose viability or get contaminated. I am not to sure how well that bag will keep it fresh and clean, but next time I would suggest using the method described by The Influence and linked to me from Riseup.
Quote:

Riseup2day said:
Quote:

BaneInc said:
Influence, is there an extended version of the syringe method you were describing?





http://www.shroomery.org/forums/showflat.php/Number/15201763#15201763

That's the whole tek to make spore syringes, but in a nutshell to sterilize the syringe:

1) Bring a pot of water to a rolling boil
2) Take empty syringe and suck up boiling water
3) Empty the water you sucked up into a sink (do not empty it back into the pot, treat the expelled water as dirty)
4) Repeat steps 2&3 5 times
5) On the last draw leave the water in the syringe and cap it.


Wait for it to cool and you can expect the syringe and the water to be sterile.




It seems to be a tried and tested method for success. i will be attempting my first shot at it this evening. Best of luck and let us know your results!


--------------------
- Bane

"Dreams are more real than reality itself, they're closer to the self."

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OfflineGalidor4
Beholder
Male


Registered: 10/10/11
Posts: 854
Last seen: 6 years, 5 months
Re: Accidental LC? [Re: BaneInc]
    #16170777 - 05/02/12 11:44 AM (12 years, 8 months ago)

Apparently this wasn't well understood. This is not the way that I get spore syringes. I have a pretty good method going, that doesn't involve that much water or a bag. That bag, it was an accident, not something I planned on. But it seems like I'll just have to syringe it up(which I know how to do). i was just wondering if there was a different method to transferring the liquid to substrate.

I know what I'm doing on Friday.

Edited by Galidor4 (05/02/12 11:45 AM)

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OfflineGalidor4
Beholder
Male


Registered: 10/10/11
Posts: 854
Last seen: 6 years, 5 months
Re: Accidental LC? [Re: Galidor4]
    #16171719 - 05/02/12 03:00 PM (12 years, 8 months ago)

If anyone here wants to see what I'm gonna do with that bag here's a link: Experiment #2

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OfflineBaneInc
Freshie
Male

Registered: 04/29/12
Posts: 26
Loc: 48.8742° N, 2.3470° E
Last seen: 12 years, 7 months
Re: Accidental LC? [Re: Galidor4]
    #16172023 - 05/02/12 04:19 PM (12 years, 8 months ago)

Oh an accidental experiment! I am all about that! Im interested to see what kind of colonization you will get with that method.


--------------------
- Bane

"Dreams are more real than reality itself, they're closer to the self."

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OfflineGalidor4
Beholder
Male


Registered: 10/10/11
Posts: 854
Last seen: 6 years, 5 months
Re: Accidental LC? [Re: BaneInc]
    #16173447 - 05/02/12 08:26 PM (12 years, 8 months ago)

Me too

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