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InvisibleBlackd0ve420
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Pre-inoculation Contam. Will Steam Sterilization Kill It?
    #16163525 - 04/30/12 11:45 PM (12 years, 8 months ago)

So I was reusing some jars I had used from my previous BRF cake tech. I washed and cleaned it with the medical alcohol. Obviously it wasn't enough because after I readied my jars yesterday (No spores in it yet) I looked at them and noticed cottony patches and a few jars containing a few yellow spots. Obviously contams since I haven't inoculated.

My question. I am currently steam sterilizing for 90 min. Will this kill the contams and allow me to inoculate? Or would I just be wasting spores and should toss the jars/substrate and steam sterilize them before filling them up again?

I am currently steam sterilizing so a quick answer would be appreciated.


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OfflineSma
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Re: Pre-inoculation Contam. Will Steam Sterilization Kill It? [Re: Blackd0ve420]
    #16163547 - 04/30/12 11:49 PM (12 years, 8 months ago)

Better safe than sorry. I say try again. Even with a pressure cooker I probably wouldn't risk it, definitely not with steam sterilization.

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InvisibleBlackd0ve420
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Re: Pre-inoculation Contam. Will Steam Sterilization Kill It? [Re: Sma]
    #16163559 - 04/30/12 11:54 PM (12 years, 8 months ago)

Yeah I suppose so. Plus just realized I didn't put tin foil on top of the jars for first 10 min of sterilizing so there will be extra moisture in the jars... :facepalm3:


I think it's a sign to start over.


--------------------
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Laugh at the world and be free,
Then Just breathe, Just breathe...
*Blackdove*


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:peace: :nyan: :peace:

Edited by Blackd0ve420 (05/01/12 12:23 AM)

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InvisibleBlackd0ve420
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Re: Pre-inoculation Contam. Will Steam Sterilization Kill It? [Re: Blackd0ve420]
    #16163603 - 05/01/12 12:09 AM (12 years, 8 months ago)

Dumped out the cakes outside(Very bad smelling). Washed them out with some soap and took the tape off. They are now being steam sterilized for an hour and a half. Hopefully this will do the trick and I'll try again tomorrow.


--------------------
Just let it all go..
Laugh at the world and be free,
Then Just breathe, Just breathe...
*Blackdove*


Click for Catnip!
:peace: :nyan: :peace:

Edited by Blackd0ve420 (05/01/12 12:09 AM)

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OfflineSma
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Re: Pre-inoculation Contam. Will Steam Sterilization Kill It? [Re: Blackd0ve420]
    #16163660 - 05/01/12 12:29 AM (12 years, 8 months ago)

Sounds good to me

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Offlinetibberous
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Re: Pre-inoculation Contam. Will Steam Sterilization Kill It? [Re: Sma]
    #16163671 - 05/01/12 12:33 AM (12 years, 8 months ago)

I should have killed everything, but some of the wastes will survive PC'ing and can give you problems (alcohol being the one that comes to mind)

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InvisibleBlackd0ve420
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Re: Pre-inoculation Contam. Will Steam Sterilization Kill It? [Re: tibberous]
    #16163691 - 05/01/12 12:38 AM (12 years, 8 months ago)

It's possible it would have worked. But I don't want to risk wasting spores. I think it was best I dumped em, I remember that smell. *shrug*. What's done is done. I think the main reason it got like that was because I waited too long to steam sterilize the substrate after putting in the jars.

I guess note to self. Sterilize ASAP otherwise you immediately start colonizing bacteria and mold due to the wet verm.


--------------------
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Then Just breathe, Just breathe...
*Blackdove*


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:peace: :nyan: :peace:

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Offlineaustink
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Re: Pre-inoculation Contam. Will Steam Sterilization Kill It? [Re: Blackd0ve420]
    #16163754 - 05/01/12 12:55 AM (12 years, 8 months ago)

Always ditch something that has a contam. Always.

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OfflineRiseup2day
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Re: Pre-inoculation Contam. Will Steam Sterilization Kill It? [Re: austink]
    #16163779 - 05/01/12 01:04 AM (12 years, 8 months ago)

Yup, you can't just pc a contaminated jar and just reinoc. Its better to start over rather than wasting time to have a contamination pop up later and waste all that time and effort.

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InvisibleBlackd0ve420
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Re: Pre-inoculation Contam. Will Steam Sterilization Kill It? [Re: Riseup2day]
    #16163839 - 05/01/12 01:22 AM (12 years, 8 months ago)

Got done steam sterilizing the jars. I think they will work this time. Next time I will IMMEDIATELY steam sterilize the substrate after filling the jars.


--------------------
Just let it all go..
Laugh at the world and be free,
Then Just breathe, Just breathe...
*Blackdove*


Click for Catnip!
:peace: :nyan: :peace:

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OfflineRogerRabbitM
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Re: Pre-inoculation Contam. Will Steam Sterilization Kill It? [Re: Blackd0ve420]
    #16164395 - 05/01/12 07:55 AM (12 years, 8 months ago)

Quote:

Next time I will IMMEDIATELY steam sterilize the substrate after filling the jars.




This is what you're supposed to do. :shrug:
RR


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InvisibleBlackd0ve420
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Re: Pre-inoculation Contam. Will Steam Sterilization Kill It? [Re: RogerRabbit]
    #16165531 - 05/01/12 12:48 PM (12 years, 8 months ago)

Quote:

RogerRabbit said:
Quote:

Next time I will IMMEDIATELY steam sterilize the substrate after filling the jars.




This is what you're supposed to do. :shrug:
RR





Yeeeaaahhh I realized that after this scenario. What happened was it was really late when I filled the jars and sort of just passed out, in my dazed mind just thinking "Ah I'll just do it tomorrow". Well least there's a lesson learned without having to waste spores. Just substrate which is cheap anyways


--------------------
Just let it all go..
Laugh at the world and be free,
Then Just breathe, Just breathe...
*Blackdove*


Click for Catnip!
:peace: :nyan: :peace:

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InvisibleBlackd0ve420
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Re: Pre-inoculation Contam. Will Steam Sterilization Kill It? [Re: Blackd0ve420]
    #16174411 - 05/02/12 10:51 PM (12 years, 8 months ago)

So after the aforementioned steam sterilizing I also let them soak in a medical sterilizing alcohol/water solution then thoroughly rinsed and dried off the jars to get rid of any alcohol residue. Put new substrate in it and steam sterilized again and let them cool off for 12 hours or less. No contams. Knocked them off 30 min ago with 1 Pf classic, 2 a+ albinos, and 2 GT's.


--------------------
Just let it all go..
Laugh at the world and be free,
Then Just breathe, Just breathe...
*Blackdove*


Click for Catnip!
:peace: :nyan: :peace:

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Invisible36fuckin5
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Re: Pre-inoculation Contam. Will Steam Sterilization Kill It? [Re: Blackd0ve420]
    #16174492 - 05/02/12 11:06 PM (12 years, 8 months ago)

Waiting overnight isn't your problem. There was something wrong with your sterilization procedure. Get a PC and use it properly and this likely won't happen again.


--------------------
Pat The Bunny said:
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bodhisatta said:
i recommend common sense and figuring it out.

These are the TEKs I use. They're all as cheap and easy as possible, just like your mom.

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InvisibleBlackd0ve420
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Re: Pre-inoculation Contam. Will Steam Sterilization Kill It? [Re: 36fuckin5]
    #16174724 - 05/02/12 11:55 PM (12 years, 8 months ago)

mmmm I think I have to go with RR on this. I mean the wet verm/flower aren't sterile when you fill them up. So 24 hours is all it needs for the contams to get a food hold in there. I did the exact same method for my first time with no problems. Did it just now with no problems so far either. Like he said I should have done it immediately. Otherwise the contams I let live will just grow out of control.


--------------------
Just let it all go..
Laugh at the world and be free,
Then Just breathe, Just breathe...
*Blackdove*


Click for Catnip!
:peace: :nyan: :peace:

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OfflineDazed Belief
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Re: Pre-inoculation Contam. Will Steam Sterilization Kill It? [Re: 36fuckin5]
    #16174734 - 05/02/12 11:57 PM (12 years, 8 months ago)

depending on what your using a day wait isnt all that bad and can be beneficial
when using my wbs i usualy make up the jars then wait a day
that one day will let more of the endospores to open up making it easier to kill bacteria
but with brf cake waiting a day or two will not help but actually hurt your jars


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im just trying to live the life.

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