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Offlinesmooflord
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Registered: 04/15/12
Posts: 40
Last seen: 12 years, 8 months
i spilled methylated spirits on my only sporeprint :(
    #16150091 - 04/28/12 03:22 AM (12 years, 8 months ago)

the print has since dried but Im curious would any spores survive?

Im very disappointed... has anyone else done this and had some spores survive, im curious to know... i prolly doubt it. fuck fuck fuck!

Edited by smooflord (04/28/12 04:19 AM)

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Invisiblehardcoresmexyou

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Registered: 02/19/12
Posts: 171
Re: i spilled methylated spirits on my only sporeprint :( [Re: smooflord]
    #16150300 - 04/28/12 06:26 AM (12 years, 8 months ago)

.

Edited by hardcoresmexyou (05/02/12 09:02 AM)

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OfflineKizzle
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Registered: 08/30/11
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Re: i spilled methylated spirits on my only sporeprint :( [Re: hardcoresmexyou]
    #16150337 - 04/28/12 06:55 AM (12 years, 8 months ago)

I'm sure it's possible that some survived but I doubt you'll find the print to be usable.


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InvisibleDoc_T
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Re: i spilled methylated spirits on my only sporeprint :( [Re: Kizzle]
    #16150393 - 04/28/12 07:51 AM (12 years, 8 months ago)

I think it will be ok to use. Try it and report back please.


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You make it all possible. Doesn't it feel good?

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Offlinesmooflord
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Registered: 04/15/12
Posts: 40
Last seen: 12 years, 8 months
Re: i spilled methylated spirits on my only sporeprint :( [Re: Doc_T]
    #16160726 - 04/30/12 02:40 PM (12 years, 8 months ago)

Sorry Doc. Its been a week and that lc is not colonizing at all. Previously when I did an lc via the same technique it fully colonized the jar in approx. 5 days.

These spores are DEAD.
I'm deeply disappointed.
They had been my precious.

I'm switching to amanitas because of this... don't trip too much anyways lol.

Still a disappointment, but its too much of a hassle to get spores!

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Offlinesmooflord
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Registered: 04/15/12
Posts: 40
Last seen: 12 years, 8 months
Re: i spilled methylated spirits on my only sporeprint :( [Re: smooflord]
    #16160749 - 04/30/12 02:45 PM (12 years, 8 months ago)

p.s I was hoping they'd survive, sorry for bumping...

Probably via capillary action they soaked up the methylated spirits and that just choked em...

It was a stupid clumsy mistake. To be honest I almost did it a second time coz I wanted to whipe the outside of the print it was on 2 projection slides, and when I picked it up I didnt notice the one side (like a hamburger sandwich) had gotten loose so I only picked up the one side with the spores on it and when I whiped it i whiped the fucking print.
It was like, OMG wtf have I done lol...

But only half of the print was swiped across like that.... but as it ends up I do recall i had whiped the outside of the discs previously a few years back and the stuff had seeped in around the edges and it got completely wet back then too, nevermind this half wipe.

How fucking DUMB to make the SAME mistake on the same sporeprint TWICE.

FUCK man but sometimes I realise I NEED TO wear my glasses lol coz I can't see batshit man!

So they're totally fuckin dead.
Im pissed but at least now I know I tried after years of being too lazy to test it coz I knew it would prolly fail I did and it did fail lol...

what else can I say...

It was a great strain even for a multispore...

So NO, SPORES DO NOT SURVIVE METHYLATED SPIRITS WHATSOEVER.

THEY FUCKIN DIE PEOPLE.

THE END.

Edited by smooflord (04/30/12 02:47 PM)

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Offlinesmooflord
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Last seen: 12 years, 8 months
Re: i spilled methylated spirits on my only sporeprint :( [Re: smooflord]
    #16160761 - 04/30/12 02:48 PM (12 years, 8 months ago)

p.s I'm gonna give it one more go this week to make double sure they're all dead...

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OfflineThe Influence
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Re: i spilled methylated spirits on my only sporeprint :( [Re: smooflord]
    #16160799 - 04/30/12 02:55 PM (12 years, 8 months ago)

Quote:

smooflord said:

I'm switching to amanitas because of this... don't trip too much anyways lol.







Amanitas are extremely hard, maybe even impossible to cultivate indoors (if thats what you were planning)

Quote:

"Amanita cultivation in a lab environment has always been an impossibility due to the symbiotic mycorrhizal relationship of this mushroom to its host trees. But if one has the necessary host trees in their area, and resides in the proper temperate zone or elevation, try and simply take a few dried or fresh caps that are in sporination (fully flattened or upturning with longitudinal tears along the striations), crush them up thoroughly, and mix the crushings into the top soil. See if it will take. If one doesn't want to make the initial investment of the caps simply chop up the stems from sporinating specimens, which will naturally have collected some of the falling spores, and mix with the soil. According to Erowid mycelia growth takes place primarily throughout the Spring and Summer months and is highly dependent on rain and soil moisture preceding the Fall fruiting. If the season is dry just water your mushroom garden every few days" (Michael S. Smith)



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OfflineDrCrumbs
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Re: i spilled methylated spirits on my only sporeprint :( [Re: The Influence]
    #16160853 - 04/30/12 03:05 PM (12 years, 8 months ago)

I'm confused your post that spilled it, was only 2.5 days ago.

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InvisibleDoc_T
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Re: i spilled methylated spirits on my only sporeprint :( [Re: DrCrumbs]
    #16161168 - 04/30/12 04:08 PM (12 years, 8 months ago)

I'm confused why LC failure = dead spores. Drop them on some agar.


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Offlinesmooflord
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Registered: 04/15/12
Posts: 40
Last seen: 12 years, 8 months
Re: i spilled methylated spirits on my only sporeprint :( [Re: Doc_T]
    #16184945 - 05/05/12 06:09 AM (12 years, 8 months ago)

naw I inoculated it a few days prior to posting...
but the same method with different spores yielded perfectly colonized lc.

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