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 makisupa09
member
Registered: 06/05/03
Posts: 105
Last seen: 20 years, 8 months
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 where the hells the colonization... 
#1615374 - 06/06/03 06:02 PM (20 years, 10 months ago) |
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ok 10 days ago i mixed together 12 1/2 pint jars of brf,verm,water- i did everything i was suppose to- boiled them for well over an hour- steralized everything- i inocculated 1cc per jar of equador cubensis- the only thing i can think of that i did wrong was when the directions said to put in 1/2 vermiculite i put in a 1/4 cup- i dont know what kind of difference this makes, but its been 10 days and theres prob 2 jars that i notice that might have a lil growth in them- i have been keeping them at a stable 80% and recently i put them in a styrophone cooler but its still at 80%, my question is what the hells going on with the colonization- has anyone ever had to wait so damn long before they notice growth- what are some things i can do to get this show on the road....
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"Once in a while you get shown the light in the strangest of places if you look at it right"
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Legoulash
Stranger
Registered: 09/07/02
Posts: 4,347
Last seen: 12 years, 9 months
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 Re: where the hells the colonization... [Re: makisupa09]
#1615395 - 06/06/03 06:15 PM (20 years, 10 months ago) |
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Shoulda followed directions... It could work but it probobly wont.. And u dont realy need to humidify during incubation...
Edited by Magic_rah (06/06/03 06:16 PM)
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 dysphoria
lost soul
Registered: 06/04/03
Posts: 1,651
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Re: where the hells the colonization... [Re: Legoulash]
#1615725 - 06/06/03 09:28 PM (20 years, 10 months ago) |
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as magic said, humidity isnt really a factor during colonization
the jars will create their own enviorment within the jar; their own temperature, and humidity.
sadly, hes right, you shoulda followed directions
using half less vermiculite, or even half less of anything in a recipe will never result in a proper substance. this is evident when you run out of flour for a birthday cake, it just wont be the same.
as for my dog, hes had jars that wont colonize...
usually these came from moisture content, some were entirely too wet, some way too dry...
and hes had jars that have started to colonize, but will stall due to moisture content.
all he sees are dehydrated mycellium spots (where it looks like a tree of sorts) (proper term?)
so this does back up peoples statement that "better to be on the dry side"
the dry jars for the most part did start to colonize, but would stop around 50%
the wet jars would either be lost to contamination quickly, or never colonize...
oh, and hes also had cakes that took upwards of two weeks before colonization started to take place in abundance. the cause? air-tight lids. the mycellium would start form, get about the size of a penny, sometimes a quarter, then just stop dead in its tracks. poking holes in his lids, then covering with aluminum foil definately made a difference for him.
just want to make the readers aware that these are just observations ive made, there could be hundreds of variables in mine, yours, and everyones setup that causes anomalies.
-jb
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makisupa09
member
Registered: 06/05/03
Posts: 105
Last seen: 20 years, 8 months
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Re: where the hells the colonization... [Re: dysphoria]
#1615743 - 06/06/03 09:38 PM (20 years, 10 months ago) |
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so from me not putting in 1/4 cup more verm. that fucked everything up
?
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"Once in a while you get shown the light in the strangest of places if you look at it right"
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lemunhed
The hustler'shustler
Registered: 05/12/03
Posts: 775
Loc: Ur moms house
Last seen: 18 years, 8 months
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Re: where the hells the colonization... [Re: makisupa09]
#1615885 - 06/06/03 11:20 PM (20 years, 10 months ago) |
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No probably not... did you put BRF in too or just the verm? And also, are you incubating that shit, because it can take a really long time to see growth without incubation.
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You're pompeius, aren't you? I'll make a pompeian of you unless you hold your toungue!
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2Experimental
Registered: 01/15/03
Posts: 18,073
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Re: where the hells the colonization... [Re: lemunhed]
#1615895 - 06/06/03 11:26 PM (20 years, 10 months ago) |
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if u had 12 jars with 1cc per jar, that would take longer for the myc to show, that is, if you injected the spores in the middle. Where did you shoot em in at?? If you did it on the side where you can see it, and you still see no growth after 10 days, i would be a little worried. My advice is this, give it 4 more days*(this is if you shot the spores in on the side)*, and after that I dunno, guess your outa luck :/
Anyways if you shot them in the middle, it could take as long as 2 weeks to see any myc poking through, and even longer if the formula wasent right
good luck dude
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photovorix
retard
Registered: 03/31/03
Posts: 211
Loc: US
Last seen: 19 years, 2 months
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Re: where the hells the colonization... [Re: 2Experimental]
#1615951 - 06/07/03 12:02 AM (20 years, 10 months ago) |
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Heh.... 2:1:1 verm:brf:water just barely mixes together all the way, 1:1:1 will NOT mix together right.... I assume he meant degrees when he said %.
Teks are written because they work, not following the tek exactly, in most cases causes it to fail.
I would guess that the reason you havent seen (much) germination is because you messed with the substrate formula. If you have to drink a half gallon of water to save your life, would you only drink 1/4 gallon?
2Experimental: The squirrel used 1cc per 1/2 pint, from a VERY light syringe, could have sworn it was just water, without an eye loop. Colonization was visible for the little nut chaser (hes a squirrel, you pervert) in 4-5 days, 24 hours in a karo jar. You are right about shooting in the middle though, took 2-3 weeks for visible colonization on the jar squirrel shot in the middle.
The squirrel recently (the 30th i believe) knocked up 3 1/2 pints with a very dark hawaiian syringe, and all are atleast 40% colonized, one is about 60% colonized. On top of the fast colonization time, he used FINE vermiculite, I have read several times here and other boards that fine will not work, colonizes slowly, etc... thats bull shit. (it worked for the squirrel 4 times counting the cambo he knocked up) All your foaf has to do is be careful not to pack it at all (can you brush your teeth? good.. you can load a jar without packing it)
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2Experimental
Registered: 01/15/03
Posts: 18,073
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Re: where the hells the colonization... [Re: photovorix]
#1615957 - 06/07/03 12:05 AM (20 years, 10 months ago) |
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wow thanks photo,afoaf will use fine verm also, its all thats available around him, glad to know it works....
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CouchLock
Stranger
Registered: 09/25/09
Posts: 21
Last seen: 13 years, 7 months
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Re: where the hells the colonization... [Re: 2Experimental]
#11132629 - 09/26/09 04:52 PM (14 years, 6 months ago) |
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heres your ingridents and how much to use of what and the steps start to finish..
http://www.fungifun.org/English/Pftek
Wont elt you down get u thru it
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dysphoria
lost soul
Registered: 06/04/03
Posts: 1,651
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Re: where the hells the colonization... [Re: CouchLock]
#11132741 - 09/26/09 05:19 PM (14 years, 6 months ago) |
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you just bumped a 6 year old thread.
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cyb3rtr0n
searching for truth
Registered: 05/06/09
Posts: 1,832
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Re: where the hells the colonization... [Re: makisupa09]
#11132756 - 09/26/09 05:20 PM (14 years, 6 months ago) |
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I would definitely toss if you haven't noticed any mycelium growth in 6 years.
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Cliffo
Stranger than you.
Registered: 09/01/08
Posts: 164
Last seen: 3 years, 3 months
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Re: where the hells the colonization... [Re: cyb3rtr0n]
#11132984 - 09/26/09 06:01 PM (14 years, 6 months ago) |
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wtf how does one manage to bump a 6 year old thread 
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fromga2wa
TRUSTED Mycolanthropist
Registered: 08/24/09
Posts: 312
Loc: Renton,WA
Last seen: 9 years, 5 months
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Re: where the hells the colonization... [Re: cyb3rtr0n]
#11133024 - 09/26/09 06:10 PM (14 years, 6 months ago) |
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HAHAHA
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DON'T CLICK HERE!!!
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beto
Psiconauta
Registered: 09/04/09
Posts: 469
Loc: Paranoid City
Last seen: 5 months, 2 days
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Re: where the hells the colonization... [Re: makisupa09]
#11133051 - 09/26/09 06:17 PM (14 years, 6 months ago) |
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Same here bro! Take a look at my grow log, today finally the mycelium showed up! At least i think it is mycelium lol
http://www.shroomery.org/forums/showflat.php/Number/11121020/page/2
It's been 8 days since inoculation! Only 1 jar out of 8 showed signs of activity! The others looks exactly the same as when i put them inside the incubator. I'll give them a week more.
Good Luck!
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veda_sticks
Cultivator
Registered: 07/29/07
Posts: 14,191
Loc: UK 
Last seen: 4 years, 3 months
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Re: where the hells the colonization... [Re: beto]
#11133255 - 09/26/09 06:55 PM (14 years, 6 months ago) |
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Gkod damn@1 I just spend ages reading this thread and its a dead thread revivall
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PF TEK - writeup by EvilMushroom666
Lets Grow Mushrooms - RogerRabbit & RoadKills website with sample videos plus the full PF TEK video series. Alot of great information - BUY THE DVD
Cakes can and will pin! - So you think cakes suck for pins. Your wrong
Franks Simple Coir/Verm Tek
Franks Proper Pasturisation Tek
Franks Spawning To Bulk - Monotub
Professor Pinheads RTV Injection Port Tek
Foo Mans No Soak WBS Prep Tek
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feelfunny
I am you
Registered: 03/11/09
Posts: 8,747
Loc: South
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Re: where the hells the colonization... [Re: veda_sticks]
#11133445 - 09/26/09 07:25 PM (14 years, 6 months ago) |
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who keeps bringing these damn old thrads up today
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IF A CAT AND DOG CAN GET ALONG WHY CANT EVERYONE ELSE?
If the sky is falling, don't look up! 
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noobieshroomie
Back again
Registered: 07/18/08
Posts: 12,769
Loc: Not Too Sure 
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Re: where the hells the colonization... [Re: feelfunny]
#11133593 - 09/26/09 07:44 PM (14 years, 6 months ago) |
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-noobie-
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AMU
Best Thread Ever
CapZilla said:
not sure what GE and FAE are but i should probably get some.
Citric said:
Your signature is wrong on colonization temps!
GOOD JUDGMENT COMES FROM EXPERIENCE
EXPERIENCE COMES FROM BAD JUDGMENT
ROOM TEMP 70-75 IS BEST FOR COLONIZATION
Thank you mycochef
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beto
Psiconauta
Registered: 09/04/09
Posts: 469
Loc: Paranoid City
Last seen: 5 months, 2 days
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Re: where the hells the colonization... [Re: noobieshroomie]
#11134103 - 09/26/09 09:14 PM (14 years, 6 months ago) |
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LOL!
I can't believe i didn't check the date!! 
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