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OfflineRiseup2day
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Registered: 03/21/12
Posts: 108
Last seen: 12 years, 7 months
Re: 17 days and not 100% colonized [Re: Lifted1]
    #16156623 - 04/29/12 06:19 PM (12 years, 8 months ago)

Bongjuice, if that is your lid design how did you inoc and still keep the tyvek intact to keep out contams?

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OfflineOH1Ogrown
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Registered: 04/23/12
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Re: 17 days and not 100% colonized [Re: Riseup2day]
    #16156714 - 04/29/12 06:42 PM (12 years, 8 months ago)

what's that red stuff and where can I find it?


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Offlinebongjuice420
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Registered: 03/12/12
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Re: 17 days and not 100% colonized [Re: Riseup2day]
    #16156733 - 04/29/12 06:48 PM (12 years, 8 months ago)

This is the lid method I used: http://www.shroomery.org/9030/Docs-Wild-Bird-Seed-Tek

I made a glovebox and used 1/4 bottle of 70% iso alcohol and rubbed that all over the box and everything around me... literally wore a paint suit made of tyvek and used a dust mask. Also had rubber gloves on. When i went to inoc the jars the cotton ball blocked my needle from poking into the jar. Its a hard way of finding out that cotton balls DONT WORK with this method! So I tore the cotton ball out of 4 jars and then inoc and on the other 4 jars I took the lid off and carefully squirted 0.5cc -1cc LC into each jar.


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All communications from this Online ID are for a fiction action book. These communications are for research purposes only and there are no intentions on implementing anything pertaining to these communications on or off this website.

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How to store mushrooms long/short-term properly - TEK
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Offlinebongjuice420
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Re: 17 days and not 100% colonized [Re: OH1Ogrown]
    #16156740 - 04/29/12 06:51 PM (12 years, 8 months ago)

Quote:

OH1Ogrown said:
what's that red stuff and where can I find it?





The red stuff is RTV SILICONE which makes a "SELF HEALING" lid. I am using that lid method next time! Also you use tyvek for the gas exchange port. Here is the link to amazons RTV SILICONE:
http://www.amazon.com/Permatex-F81160-Hi-Temp-Silicone--3-Ounce/dp/B0002UEN1A/ref=sr_1_2?ie=UTF8&qid=1335746922&sr=8-2

Also here is the link on instructions on how to make this type of lid: http://www.shroomery.org/forums/showflat.php/Number/10628004#10628004


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All communications from this Online ID are for a fiction action book. These communications are for research purposes only and there are no intentions on implementing anything pertaining to these communications on or off this website.

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How to store mushrooms long/short-term properly - TEK
Golden Teacher Time-Lapse Mono-Tub

:mxe::sporedrop: :pantytail:  :nicesmile:  :chalkup:  :sexymeow:

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Invisiblehardcoresmexyou

Folding@home Statistics
Registered: 02/19/12
Posts: 171
Re: 17 days and not 100% colonized [Re: bongjuice420]
    #16156762 - 04/29/12 06:55 PM (12 years, 8 months ago)

.

Edited by hardcoresmexyou (05/02/12 08:57 AM)

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OfflineLifted1
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Re: 17 days and not 100% colonized [Re: hardcoresmexyou]
    #16156811 - 04/29/12 07:06 PM (12 years, 8 months ago)

Cotton is not a good filter, that's why we use synthetic poly fil. Cotton is nutritious. If all you have is poly fil, tyvek and pore tape, make a lid like you see in my second pic. Works fine. I drilled a 3/8" hole for ge, stuffed with polyfil. I drilled a 1/4" hole for injection. I took a piece of tyvek slightly bigger than my hole, and pore taped it on, with two layers. After injection, reapply 2 layers of tape. I also tightened my lid down onto tyvek as well, but I'm not sure that's necessary. I know it doesn't hurt tho.


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Discovering yourself is only "discovery" in so much as you discovered that what you once thought was real, is only a mirage.

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OfflineOH1Ogrown
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Re: 17 days and not 100% colonized [Re: Lifted1]
    #16156990 - 04/29/12 07:49 PM (12 years, 8 months ago)

Awesome! self healing... like stretch Armstrong?
Has anybody ever used a piece of Stretch Armstrong for an injector port?

GANGSTER!!!!!!!!
LMAO


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Offlinejointroller420
Alchemist


Registered: 12/15/11
Posts: 60
Last seen: 11 years, 8 months
Re: 17 days and not 100% colonized [Re: OH1Ogrown]
    #16157352 - 04/29/12 08:56 PM (12 years, 8 months ago)

You need to smoke a bowl, sit your ass down and wait.  :pipesmoke:  Give them time.


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:discorex::discorex::discorex:

Open the door

Get on the floor

Everybody walk

The dinosaur

:discorex::discorex::discorex:

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Offlinebongjuice420
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Registered: 03/12/12
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Re: 17 days and not 100% colonized [Re: jointroller420]
    #16157860 - 04/29/12 10:50 PM (12 years, 8 months ago)

Quote:

jointroller420 said:
You need to smoke a bowl, sit your ass down and wait.  :pipesmoke:  Give them time.






Auctually, I did lol. I looked at them tonight and I'm not 100% positive but it looks like there is fresh mycelien growth along the jar. Maybe shaking them helped???


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All communications from this Online ID are for a fiction action book. These communications are for research purposes only and there are no intentions on implementing anything pertaining to these communications on or off this website.

GT - Monotub / Time-Lapse
How to store mushrooms long/short-term properly - TEK
Golden Teacher Time-Lapse Mono-Tub

:mxe::sporedrop: :pantytail:  :nicesmile:  :chalkup:  :sexymeow:

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Offlineelkart
Mushroom Servant
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Registered: 01/28/12
Posts: 1,170
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Re: 17 days and not 100% colonized [Re: bongjuice420]
    #16157872 - 04/29/12 10:52 PM (12 years, 8 months ago)

That syringe appeared to have been unshaken. Is that right? That could be part of the problem


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Shroom Honey Preservation Tek

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Offlinebongjuice420
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Re: 17 days and not 100% colonized [Re: elkart]
    #16157899 - 04/29/12 10:58 PM (12 years, 8 months ago)

Quote:

elkart said:
That syringe appeared to have been unshaken. Is that right? That could be part of the problem





I shook it really really good in between each jar. I'm thinking the jars either stalled, are slow growing, or the white is some cobweb contam.


--------------------
All communications from this Online ID are for a fiction action book. These communications are for research purposes only and there are no intentions on implementing anything pertaining to these communications on or off this website.

GT - Monotub / Time-Lapse
How to store mushrooms long/short-term properly - TEK
Golden Teacher Time-Lapse Mono-Tub

:mxe::sporedrop: :pantytail:  :nicesmile:  :chalkup:  :sexymeow:

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Offlinebongjuice420
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Re: 17 days and not 100% colonized [Re: bongjuice420]
    #16161131 - 04/30/12 03:59 PM (12 years, 8 months ago)

I think shaking them helped. The myc is growing exponentially!!!


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All communications from this Online ID are for a fiction action book. These communications are for research purposes only and there are no intentions on implementing anything pertaining to these communications on or off this website.

GT - Monotub / Time-Lapse
How to store mushrooms long/short-term properly - TEK
Golden Teacher Time-Lapse Mono-Tub

:mxe::sporedrop: :pantytail:  :nicesmile:  :chalkup:  :sexymeow:

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OfflineDrCrumbs
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Registered: 10/25/11
Posts: 1,619
Loc: Gili Trawangan
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Re: 17 days and not 100% colonized [Re: bongjuice420]
    #16161353 - 04/30/12 04:51 PM (12 years, 8 months ago)

The more the myc was distributed evenly from the shake will really help. Yeah once they recover after that shake and they are gonna take off.

17 days is no big deal and starting from spores and not an LC its really not surprising. Myc grows exponentially. They will look great in a week and probably be ready to get transferred to some substrate in two.

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InvisibleNewfoundFreedom
Manure whore


Registered: 05/06/04
Posts: 1,915
Re: 17 days and not 100% colonized [Re: DrCrumbs]
    #16161685 - 04/30/12 05:52 PM (12 years, 8 months ago)

That's an outdated and poor "TEK" you're following, OP.

"Shake: Once you inoculate all of the jars, take and shake each one vigorously to distribute the spores throughout the WBS "

Bad advice. Also, coffee filters and usps tyvek envelopes are crappy material for GE filters.

Edited by NewfoundFreedom (04/30/12 05:53 PM)

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OfflineLifted1
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Re: 17 days and not 100% colonized [Re: NewfoundFreedom]
    #16162093 - 04/30/12 06:57 PM (12 years, 8 months ago)

I'm gonna second the coffee filter thing. When I started, I read the coffee filter thing and used it for awhile, til I realized it was a major factor in my failures. Coffee filters = suck.


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Discovering yourself is only "discovery" in so much as you discovered that what you once thought was real, is only a mirage.

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OfflineOH1Ogrown
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Registered: 04/23/12
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Re: 17 days and not 100% colonized [Re: Lifted1]
    #16162226 - 04/30/12 07:19 PM (12 years, 8 months ago)

Using a coffee filter for GE? Sounds like you might as well be using a slice of bread.


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OfflineLifted1
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Re: 17 days and not 100% colonized [Re: OH1Ogrown]
    #16162253 - 04/30/12 07:25 PM (12 years, 8 months ago)

Well to be clear, I'm not sure the coffee filters are meant for ge. When I used them, per some dumb tek I saw, there was a polyfil ge filter, and coffee filter was placed over lids before tin foil an pc'd. Coffee filters remained on. I'm pretty sure all it ever served me was to wick moisture up the coffee filter and onto my ge filter.


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Discovering yourself is only "discovery" in so much as you discovered that what you once thought was real, is only a mirage.

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Offlinebongjuice420
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Registered: 03/12/12
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Re: 17 days and not 100% colonized [Re: Lifted1]
    #16163304 - 04/30/12 10:52 PM (12 years, 8 months ago)

Theres no coffee filter on my jars. Only USPS tyvek with a hole in it from the needle lol. So how often should the jars be shaken and whats a good grain prep to follow?


--------------------
All communications from this Online ID are for a fiction action book. These communications are for research purposes only and there are no intentions on implementing anything pertaining to these communications on or off this website.

GT - Monotub / Time-Lapse
How to store mushrooms long/short-term properly - TEK
Golden Teacher Time-Lapse Mono-Tub

:mxe::sporedrop: :pantytail:  :nicesmile:  :chalkup:  :sexymeow:

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OfflineLifted1
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Re: 17 days and not 100% colonized [Re: bongjuice420]
    #16163350 - 04/30/12 11:01 PM (12 years, 8 months ago)

Quote:

bongjuice420 said:
Theres no coffee filter on my jars. Only USPS tyvek with a hole in it from the needle lol. So how often should the jars be shaken and whats a good grain prep to follow?




tyvek with hole? did you cover it with pore tape after knock up?  be specific.

Grain prep.. rinse grains till semi clear ( 3 rinses for rye? ) soak 20 ish hours with gypsum. Rinse? bring to boil for a couple minutes then pour to strainer and steam dry for like 10 minutes til dry. PC 1.5 hours or more.


--------------------

Discovering yourself is only "discovery" in so much as you discovered that what you once thought was real, is only a mirage.

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Offlinebongjuice420
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Re: 17 days and not 100% colonized [Re: Lifted1]
    #16172065 - 05/02/12 04:27 PM (12 years, 8 months ago)

What do you think of my jars now? How many % of the jars do you think are colonized.



--------------------
All communications from this Online ID are for a fiction action book. These communications are for research purposes only and there are no intentions on implementing anything pertaining to these communications on or off this website.

GT - Monotub / Time-Lapse
How to store mushrooms long/short-term properly - TEK
Golden Teacher Time-Lapse Mono-Tub

:mxe::sporedrop: :pantytail:  :nicesmile:  :chalkup:  :sexymeow:

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