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OfflinePapa Papaya
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Registered: 03/06/12
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Last seen: 3 years, 6 months
1st grow, what did I do wrong?
    #16147086 - 04/27/12 01:47 PM (12 years, 8 months ago)

So this is my first grow, I inoculated 33 1/2 pint jars about 6 weeks ago following BRF Tek to a T. 9 were B+, 12 were P.E.6 and the other 11 were Mazatapec. The P.E.6's are taking forever to colonize, but the B+ and the Mazas went fairly quickly and seemed to have no infection. I birthed 10 of the cakes about a week ago and put them in the fruiting chamber. The B+ look fine, but still no pinning. The Mazas did something weird, another hard white growth started coming out of them. I wasn't sure what it was at first, and it was the same color and appearance as the rest of the cake, imo, so I let it go. This morning I got up and all this extra growth has turned bluish green and just looks funky. I was already pretty worried about it.
Can someone please tell me what this is and how I can prevent it next time? It's only present in the Mazas, not the B+.
I'm assuming these cakes are garbage at this point, but I'll wait to hear some responses before I toss them. Thanks.


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Offlinebooyaga
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Re: 1st grow, what did I do wrong? [Re: Papa Papaya]
    #16147256 - 04/27/12 02:24 PM (12 years, 8 months ago)

I think you have green mould my friend..


--------------------
"I'm not here to stir any shit up, I don't do that. I'm not a mind-fucker, I'm an ass-kicker"
Brock Lesnar

"We are what we repeatedly do. Excellence, then, is not an act, but a habit." -Aristotle

Do I believe in aliens? Yes I do, I've actually seen them. They hang out in the parking lot of Home Depot.

:frankly:

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OfflineTheDeathryder
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Re: 1st grow, what did I do wrong? [Re: booyaga]
    #16147346 - 04/27/12 02:47 PM (12 years, 8 months ago)

Definately looks like mold.  I'd toss them into the compost pile just to be safe.

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Offline420greenery
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Re: 1st grow, what did I do wrong? [Re: booyaga]
    #16147353 - 04/27/12 02:49 PM (12 years, 8 months ago)

Quote:

booyaga said:
I think you have green mould my friend..



:batpole: "where is the pole?!"

All I saw is a ton of green mold on those cakes. I would suggest tossing those before risking the lives of the others.

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InvisibleEvilMushroom666
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Re: 1st grow, what did I do wrong? [Re: Papa Papaya]
    #16147368 - 04/27/12 02:52 PM (12 years, 8 months ago)

Those cakes are contaminated, either from a lapse in sterile procedure,
preparation of the cakes, of a contaminated syringe.

As others have suggested dump them outside in the compost bin, and try
try again.

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OfflinePapa Papaya
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Re: 1st grow, what did I do wrong? [Re: EvilMushroom666]
    #16147965 - 04/27/12 04:59 PM (12 years, 8 months ago)

ok, to the compost bin they go. Thanks everyone.
They looked fine in the jar, solid white, no discoloration. Is this something that could have happened during the dunk and roll? I used tap water and an unopened bag of sterile vermiculite.
Any reason only the mazas would have it? infected syringe maybe? or just a strain-specific weakness?

I have 7 or 8 more jars to birth, just wondering if I'm screwed on all of them, or if I could maybe salvage the rest?

Also, they just turned color overnight. They've been having those large structures protruding from the cakes for about a week, but they were solid white. Does that sound like mold, or is there possibly an additional infection?

Guess I've got to work on my sterile procedure, but sheesh, none of the jars showed infection until after birthing. I was under the impression that they were pretty resistant to infection once fully colonized, maybe I'm totally wrong on this?

Thanks everyone.

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OfflinePapa Papaya
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Re: 1st grow, what did I do wrong? [Re: Papa Papaya]
    #16148033 - 04/27/12 05:15 PM (12 years, 8 months ago)

Here's a photo of what they looked like a few days ago, before the mold, but long after I was suspicious of infection. Maybe some of you guys recognize this growth?




I know none of this has any bearing on what i need to do at this point (i.e. start over/sterile procedure) but I'm still pretty curious whether this type of growth is natural or not. Should there be anything but mushrooms protruding from my cakes?

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InvisibleEvilMushroom666
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Re: 1st grow, what did I do wrong? [Re: Papa Papaya]
    #16148049 - 04/27/12 05:17 PM (12 years, 8 months ago)

The infection was likely from your syringe or procedure, the mycelium
of the contaminate was established inside the jar before you dunked IMO.

On those cakes I do notice healthy cube mycelium, but also can see
the contaminate.

In the pictures you just posted those large fluffy marshmellow like
growths are the pre-sporulating mycelium of trichoderma or other contaminate.

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InvisibleEvilMushroom666
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Re: 1st grow, what did I do wrong? [Re: EvilMushroom666]
    #16148056 - 04/27/12 05:19 PM (12 years, 8 months ago)

Did you sterilize your needle between jars, how did you sterilize your
cakes(method, duration etc), the more information you give us the better
chance we have of identifying the vector of contamination.

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OfflinePapa Papaya
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Re: 1st grow, what did I do wrong? [Re: EvilMushroom666]
    #16150230 - 04/28/12 05:33 AM (12 years, 8 months ago)

Oh, whoops, I thought I posted this last night.
Anyway, I PC'ed my jars for 90 minutes at 15 psi, I flamed my needle between jars, used a glove box to be safe, though not one with positive pressure, never let the needle touch anything except the sterile medium inside the jar. Basically if it's on the BRF tek (which all that is) I followed it. Guessing it must have been the syringe, since my others were not infected. It was a freebie syringe, so I probably can't complain too much.

Do you think it's possible to spot this infection still in the jar? I have quite a few more jars from the same syringe and inoculation session. The jars do look a little different from the P.E.6 and the B+, but I figured it was just a expression of phenotype. I'll upload photos later today.

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OfflineKizzle
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Re: 1st grow, what did I do wrong? [Re: Papa Papaya]
    #16150331 - 04/28/12 06:49 AM (12 years, 8 months ago)

It should be noticeable before the jar finishes, usually even changing colors before then. If it seems to be growing particularly fast that alone can be a sign it's mold


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InvisibleXxJason LivesxX

Registered: 04/09/12
Posts: 103
Re: 1st grow, what did I do wrong? [Re: Papa Papaya]
    #16150362 - 04/28/12 07:16 AM (12 years, 8 months ago)

Shit I got a syringe of maz as a freebie this month too. I hope they're not all fubar. Can you pm methe vendor.

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OfflinePapa Papaya
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Re: 1st grow, what did I do wrong? [Re: XxJason LivesxX]
    #16150441 - 04/28/12 08:33 AM (12 years, 8 months ago)

There was definitely absolutely no discoloration before birthing, and still none until over a week afterward. The jars did colonize the fastest technically, but the difference between them and the B+ was negligible.
The fluffy protrusions began immediately after birthing (noticeable within 24 hrs).

These jars have a particularly fibrous appearance, the strands are like big ropes...screw it, I'm gonna get some photos real quick...




Only the mazas have this appearance. For future reference, is this a sign of an infected jar?

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OfflinePapa Papaya
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Re: 1st grow, what did I do wrong? [Re: Papa Papaya]
    #16164361 - 05/01/12 07:34 AM (12 years, 8 months ago)

YES! Got 'em!



Nothing too impressive, but being that these are the first mushrooms I've ever grown I'm pretty super excited. These are B+.

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Offlinelsatrap
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Re: 1st grow, what did I do wrong? [Re: Papa Papaya]
    #16164501 - 05/01/12 08:35 AM (12 years, 8 months ago)

Congrats on finally getting some fruits.. Keep it up
  Learn how to make prints from your own shrooms. It really isn't hard to do.


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At this moment you should be with us, feeling like we do.


               

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OfflinePapa Papaya
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Re: 1st grow, what did I do wrong? [Re: lsatrap]
    #16169681 - 05/02/12 05:18 AM (12 years, 8 months ago)

Yeah I'm planning on making a print of this first one to open as soon as I can so I can get 30 more jars coming along. I think I got in over my head with the different strains and for now I'll just stick to B+. I have a print of the P.E.6 so I can go ahead with that whenever I think I'm ready.

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OfflinePapa Papaya
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Re: 1st grow, what did I do wrong? [Re: Papa Papaya]
    #16169687 - 05/02/12 05:23 AM (12 years, 8 months ago)

I'm seeing these fruits because I finally started giving them enough FAE. I've been swamped with a massive outdoor guerilla grow I'm working on, among other things, so my attention has been stretched really thin and I neglected the healthy cakes because I was so concerned about the unhealthy ones.

I'm thinking about getting a little PC fan and putting it on a timer to give them FAE for me, so I don't have to be here to air the thing out 5 times a day. Is this something that people do, or would I get the same FAE turning my chamber into a shotgun? (I bought this chamber and most of my supplies used on craigslist, so I'm just using the chamber as it came, though I've heard shotgun is the easiest way to go)

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Offlinelsatrap
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Re: 1st grow, what did I do wrong? [Re: Papa Papaya]
    #16171321 - 05/02/12 01:32 PM (12 years, 8 months ago)

I personally don't like shotgun tubs but I'm around to fan mine regularly.  Yes people do put small fans in the same room as shotgun chamber on low.  Timer is a good idea so it doesn't dry out perlite.Don't place it to close to sgfc though.


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At this moment you should be with us, feeling like we do.


               

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InvisibleZeet
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Re: 1st grow, what did I do wrong? [Re: lsatrap]
    #16171760 - 05/02/12 03:09 PM (12 years, 8 months ago)

I think he meant a computer fan. If you check out the behind of a desktop computer or even your laptop. One of those small fans.

I'm curious.. as I have a few lying about. How would you attack it? Just screw it on the side of the tub? I only see one problem, you're going to constantly have a hole left open to contams. As you'd have to cut a hole for the fan to pull air from.

My idea: Cut a hole, apply some tyvek/piece of rigid felt (synthetic felt stuff) and then stick the fan on. Might work. You'd have to drill holes on the opposite side of the box though, so when the fan goes on it will pull air through those holes and out through the fan.

What do you think?

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OfflinePapa Papaya
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Re: 1st grow, what did I do wrong? [Re: Zeet]
    #16172135 - 05/02/12 04:41 PM (12 years, 8 months ago)

Sounds like it would work for you, but I've already got holes drilled on 4 sides of my fruiting chamber. Like I said, I bought all my stuff from a guy on craigslist and this is the way he did it. There are no holes in the bottom or top of the chamber, tho.
But it's sounding like mounting a small pc fan will be my more reliable option. I would just trace the fan on the lid of the chamber, then cut out the hole, making sure it fits snug. Then I'd just drop it in, why seal?
Would that be a mistake? Do most of you guys grow in sealed containers? I didn't think it was necessary.

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