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Invisiblecc2
Mush

Registered: 05/15/10
Posts: 2,611
Agar contams poll ! * 1
    #13458702 - 11/09/10 12:23 PM (13 years, 2 months ago)

please fill the poll!

let us know about your agar contaminants! :smile:
After how many hours do you check your freshly poured agar for contams?
You may choose only one
At which temperature do you store your freshly poured plates waiting to check for contaminants?
You may choose only one
What is your most common contaminant?
You may choose only one
Which agar recipe do you use most frequently?
You may choose only one


Votes accepted from (11/09/10 12:23 PM) to (No end specified)
You must vote before you can view the results of this poll



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InvisibleGetafixx
NotaRoman
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Registered: 05/10/09
Posts: 524
Loc: 2nd Continent on the Left
Re: Agar contams poll ! [Re: cc2]
    #13462771 - 11/10/10 07:22 AM (13 years, 2 months ago)

Not many contams from pouring agar maybe 5 in 100 go bad.
Contams on my agar happen when inoculations are made with some traded prints.
The best advice i can give anyone is get/build a Good GloveBox.


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Invisibleandymc
cocoa beetles from zanzibar
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Registered: 11/25/09
Posts: 2,395
Re: Agar contams poll ! [Re: Getafixx]
    #13463094 - 11/10/10 09:33 AM (13 years, 2 months ago)

Why did you PM me about this poll?  Do I know you?


--------------------
How I make spore prints
Trade List
My flow hood

If he asks me "Did you have a good time?" I'll say,
"Get the lights, Mr. Grim Reaper"  -odds


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OfflinePitcherCrab
Crescent Fresh
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Registered: 12/02/06
Posts: 2,446
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Re: Agar contams poll ! [Re: andymc]
    #13463145 - 11/10/10 09:56 AM (13 years, 2 months ago)

I don't really wait to check for contams, because I don't usually get them on fresh plates. After my poured plates have been sitting around for months I sometimes get a few that go bad, but generally they are still good even then. Maybe my glove box is just that awesome?


--------------------
PC's LAGM 2021 (TOC & TWC)
P. natalensis Growlog 2021
Pans for PC Fall 2021 Growlog

“I must not fear. Fear is the mind-killer. Fear is the little death that brings total obliteration. I will face my fear. I will permit it to pass over me and through me. And when it has gone past I will turn the inner eye to see its path. Where the fear has gone there will be nothing. Only I will remain.” - Bene Gesserit Litany Against Fear



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Invisiblemycoelf
Agent Of Chaos
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Registered: 06/26/09
Posts: 555
Loc: hyperspace Flag
Re: Agar contams poll ! [Re: PitcherCrab]
    #13463174 - 11/10/10 10:09 AM (13 years, 2 months ago)

I store my plates a room temp and maybe get one in 1000, maybe that contams, and if it does you can bet that a case of the butterfingers occurred while pouring. Any questionable plates are isolated in sandwich baggies for observation.


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Invisiblejokefox
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Registered: 12/22/09
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Re: Agar contams poll ! [Re: mycoelf]
    #13463748 - 11/10/10 12:16 PM (13 years, 2 months ago)

i use antibiotic PDA ,

thats why i dont have any
shits expensive to put together lol


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Offlinewhitelight7
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Registered: 02/17/09
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Re: Agar contams poll ! [Re: jokefox]
    #13463883 - 11/10/10 12:46 PM (13 years, 2 months ago)

I don't check for contams either, just inoculate.  Pour in front of flowhood and there's no need.


--------------------
Staunch supporter of spontaneous generation:cheers:
           


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Invisiblecc2
Mush

Registered: 05/15/10
Posts: 2,611
Re: Agar contams poll ! [Re: whitelight7]
    #13464220 - 11/10/10 03:48 PM (13 years, 2 months ago)

I'm curious if those users voting "other" in the first question didn't accept 68°F as a standard room temperature or keep them at higher temps.

also, which other agar formulas are used? I think I listed the common ones, lacking just PDYA and Saboraud.


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Offlinex7x_x7x
x7x, my problem child.
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Re: Agar contams poll ! [Re: cc2]
    #13464789 - 11/10/10 05:37 PM (13 years, 2 months ago)

i store at room temp and no check for contams... i use little jars with standard lids instead of petri dishes.


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cultivando en la miseria

SuctoSpore® Pictorial Tek


x7x_x7x@shroomery.org

carl_jung_in_lsd@yahoo.com

koh samui and oak ridge are my favourite strains


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InvisibleDoc_T
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Re: Agar contams poll ! [Re: andymc] * 1
    #13464819 - 11/10/10 05:43 PM (13 years, 2 months ago)

Quote:

andymc said:
Why did you PM me about this poll?  Do I know you?




You're one of the cool kids. :wink:

Note there's no STAL option; this poll is invalid by current Shroomery standards.


--------------------
You make it all possible. Doesn't it feel good?


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OfflineMaverick
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Re: Agar contams poll ! [Re: Doc_T] * 1
    #13478948 - 11/13/10 03:24 PM (13 years, 2 months ago)

Quote:

Doc_T said:
Quote:

andymc said:
Why did you PM me about this poll?  Do I know you?




You're one of the cool kids. :wink:

Note there's no STAL option; this poll is invalid by current Shroomery standards.




Also, what about us who haven't done enough agar, but want to vote sometime in the future but still want to see results? :frown:


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Invisiblecc2
Mush

Registered: 05/15/10
Posts: 2,611
Re: Agar contams poll ! [Re: Maverick]
    #13479052 - 11/13/10 03:46 PM (13 years, 2 months ago)

results influence your vote, so better not lookin' at them first :jellyfish:


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OfflineMaverick
Lover of Earwigs!
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Posts: 13,437
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Re: Agar contams poll ! [Re: cc2]
    #13479192 - 11/13/10 04:18 PM (13 years, 2 months ago)

I casted a blank vote and you will now never know my opinion on the matter, because by the time I have one developed, this thread will be old news.


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Invisiblecc2
Mush

Registered: 05/15/10
Posts: 2,611
Re: Agar contams poll ! [Re: Maverick]
    #13482321 - 11/14/10 11:00 AM (13 years, 2 months ago)

Quote:

DRTMaverick said:
I casted a blank vote and you will now never know my opinion on the matter




could live with that :tongue2:


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OfflineBig_Sexy
LoveMonger
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Registered: 10/04/11
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Last seen: 11 years, 1 month
Re: Agar contams poll ! [Re: cc2]
    #16063265 - 04/08/12 10:35 PM (11 years, 9 months ago)

Nice thread, want this baby in my list!


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Invisibleandymc
cocoa beetles from zanzibar
Male User Gallery


Registered: 11/25/09
Posts: 2,395
Re: Agar contams poll ! [Re: Big_Sexy]
    #16066539 - 04/09/12 06:06 PM (11 years, 9 months ago)

Quote:

Big_Sexy said:
Nice thread, want this baby in my list!



:nonono::oldthread:


--------------------
How I make spore prints
Trade List
My flow hood

If he asks me "Did you have a good time?" I'll say,
"Get the lights, Mr. Grim Reaper"  -odds


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OfflineBig_Sexy
LoveMonger
 User Gallery


Registered: 10/04/11
Posts: 602
Last seen: 11 years, 1 month
Re: Agar contams poll ! [Re: andymc]
    #16066658 - 04/09/12 06:32 PM (11 years, 9 months ago)

Just because its old doesn't mean its not good, hell thermodynamics hasn't changed since the 1940's. What you're recommending I repost this myself?  I don't get it?


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OfflineNalim
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Registered: 01/13/06
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Last seen: 1 year, 3 months
Re: Agar contams poll ! [Re: whitelight7]
    #16137239 - 04/25/12 08:33 AM (11 years, 9 months ago)

Quote:

whitelight7 said:
I don't check for contams either, just inoculate.  Pour in front of flowhood and there's no need.




--------------------

Rodney Brooks on Robots
Nalim said: "Quoting yourself is retarded."


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